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Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics

  • Esthelle Hoedt
  • Guoan Zhang
  • Thomas A. NeubertEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1140)

Abstract

Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach for high-throughput quantitative proteomics. SILAC allows highly accurate protein quantitation through metabolic encoding of whole cell proteomes using stable isotope labeled amino acids. Since its introduction in 2002, SILAC has become increasingly popular. In this chapter we review the methodology and application of SILAC, with an emphasis on three research areas: dynamics of posttranslational modifications, protein-protein interactions, and protein turnover.

Keywords

Stable isotope labeling by amino acids in cell culture (SILAC) Metabolic labeling Quantitative proteomics Mass spectrometry 

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Esthelle Hoedt
    • 1
  • Guoan Zhang
    • 2
  • Thomas A. Neubert
    • 1
    Email author
  1. 1.Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell BiologyNew York University School of MedicineNew YorkUSA
  2. 2.Proteomics and Metabolomics Core FacilityWeill Cornell MedicineNew YorkUSA

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