Natural Value-Added Compounds from Fungal Communities

  • Paramanantham Parasuraman
  • Busi Siddhardha
Part of the Fungal Biology book series (FUNGBIO)


Since ancient times, fungi and fungal products have gained noticeable attention for pharmaceutical applications and even for human consumption. Recent research has provided significant evidence of fungal metabolites with a wide variety of biological activities. The bioactive compounds from fungal communities are exploited by industrial sectors from the basic biomass-based food and feed application as nutraceutials to high-value pharmaceutical, cosmeceutical, ecological, and biomedical applications. Recent advances in scientific knowledge include interest in the use of natural value-added products, especially from fungi. Fungal communities provide highly diversified value-added products with complex chemical structures impossible or difficult to produce by chemical synthetic methods. Recent efforts have explored novel endophytic fungi which are added to the list of potential providers of value-added products. Functional genomics and proteomics facilitate the isolation and production of novel metabolites from fungi. Genetic engineering is an useful tool to metabolically engineer the microorganism for the production of value-added compounds. Various high-throughput screening methods are gaining importance for the rapid detection and isolation of bioactive compounds from fungi.


Biofuels Biomedical application Genetic engineering Microbial synthesis Natural products Value-added products 



We are grateful to Pondicherry University for its support in providing the facility to access the different international journals for the successful completion of this chapter.


  1. Amirkia V, Heinrich M (2014) Alkaloids as drug leads: a predictive structural and biodiversity-based analysis. Phytochem Lett 10:43–53CrossRefGoogle Scholar
  2. Balasundram N, Sundram K, Samman S (2006) Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem 99(1):191–203CrossRefGoogle Scholar
  3. Barman S, Sit N, Badwaik LS, Deka SC (2014) Pectinase production by Aspergillus niger using banana (Musa balbisiana) peel as substrate and its effect on clarification of banana juice. J Food Sci Technol 43:591–595Google Scholar
  4. Bond C, Tang Y, Li L (2016) Saccharomyces cerevisiae as a tool for mining, studying and engineering fungal polyketide synthases. Fungal Genet Biol 89(3):52–61PubMedPubMedCentralCrossRefGoogle Scholar
  5. Burruano S, Giambra S, Mondello V, Dellagreca M, Basso S, Angela T, Andolfi A (2016) Naphthalenone polyketides produced by Neofusicoccum parvum, a fungus associated with grapevine Botryosphaeria dieback. Phytopathol Mediterr 55:197–206Google Scholar
  6. Caputi L, Aprea E (2011) Use of terpenoids as natural flavouring compounds in food industry. Recent Pat Food Nutr Agric 3(1):9–16PubMedCrossRefGoogle Scholar
  7. Chandrasekaran M, Sathiyabama M (2014) Production, partial purification and characterization of protease from a phytopathogenic fungi Alternaria solani (Ell. and Mart.) Sorauer. J Basic Microbiol 54(8):763–774PubMedCrossRefGoogle Scholar
  8. Chandrasekaran S, Kumaresan S, Manavalan M (2015) Production and optimization of protease by filamentous fungus isolated from paddy soil in Thiruvarur District Tamilnadu. J Appl Biol Biotechnol 3(6):66–69Google Scholar
  9. da Silva VC, Rodrigues CM (2014) Natural products: an extraordinary source of value-added compounds from diverse biomasses in Brazil. Chem Biol Technol Agric 1(1):14CrossRefGoogle Scholar
  10. de Souza JJ, Vieira IJC, Rodrigues-Filho E, Braz-Filho R (2011) Terpenoids from endophytic fungi. Molecules 16(12):10604–10618PubMedPubMedCentralCrossRefGoogle Scholar
  11. Daley DK, Brown KJ, Badal S (2017) Chapter 20 - Fungal metabolites. In: Badal S, Delgoda RBT-P (eds) Pharmacognosy. Academic Press, Boston, pp 413–421CrossRefGoogle Scholar
  12. Deepika VB, Murali TS, Satyamoorthy K (2016) Modulation of genetic clusters for synthesis of bioactive molecules in fungal endophytes: a review. Microbiol Res 182:125–140PubMedCrossRefGoogle Scholar
  13. Du J, Shao Z, Zhao H (2011) Engineering microbial factories for synthesis of value-added products. J Ind Microbiol Biotechnol 38(8):873–890PubMedPubMedCentralCrossRefGoogle Scholar
  14. Fujii I (2010) Functional analysis of fungal polyketide biosynthesis genes. J Antibiot (Tokyo) 63(5):207–218CrossRefGoogle Scholar
  15. Gao Z, Li B, Zheng C, Wang G (2008) Molecular detection of fungal communities in the Hawaiian marine sponges Suberites zeteki and Mycale armata. Appl Environ Microbiol 74(19):6091–6101PubMedPubMedCentralCrossRefGoogle Scholar
  16. Garrido-Arandia M, Silva-Navas J, Ramírez-Castillejo C, Cubells-Baeza N, Gómez-Casado C, Barber D, Díaz-Perales A (2016) Characterisation of a flavonoid ligand of the fungal protein Alt a 1. Sci Rep 6(1):33468PubMedPubMedCentralCrossRefGoogle Scholar
  17. Geoffry K, Achur RN (2018) Screening and production of lipase from fungal organisms. Biocatal Agric Biotechnol 14(November 2017):241–253CrossRefGoogle Scholar
  18. Gong JS et al (2012) Nitrilases in nitrile biocatalysis: recent progress and forthcoming research. Microb Cell Fact 11:142PubMedPubMedCentralCrossRefGoogle Scholar
  19. Griebeler N, Polloni AE, Remonatto D, Arbter F, Vardanega R, Cechet JL, Ninow JL (2011) Isolation and screening of lipase-producing fungi with hydrolytic activity. Food Bioprocess Tech 4(4):578–586CrossRefGoogle Scholar
  20. Guedes AC, Amaro HM, Malcata FX (2011) Microalgae as sources of high added-value compounds: a brief review of recent work. Biotechnol Prog 27(3):597–613PubMedCrossRefGoogle Scholar
  21. Gupta RK, Gangoliya SS, Singh NK (2014) Isolation of thermotolerant phytase producing fungi and optimisation of phytase production by Aspergillus niger NRF9 in solid state fermentation using response surface methodology. Biotechnol Bioprocess Eng 19:996–1004CrossRefGoogle Scholar
  22. Hassan S, Mathesius U (2012) The role of flavonoids in root-rhizosphere signalling: opportunities and challenges for improving plant-microbe interactions. J Exp Bot 63(9):3429–3444PubMedCrossRefGoogle Scholar
  23. Huang W-Y, Cai Y-Z, Zhang Y (2009) Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr Cancer 62(1):1–20CrossRefGoogle Scholar
  24. Hussain G, Rasul A, Anwar H, Aziz N, Razzaq A, Wei W, Li X (2018) Role of plant derived alkaloids and their mechanism in neurodegenerative disorders. Int J Biol Sci 14(3):341–357PubMedPubMedCentralCrossRefGoogle Scholar
  25. Imran M, Anwar Z, Irshad M, Asad MJ, Ashfaq H (2016) Cellulase production from species of fungi and bacteria from agricultural wastes and its utilization in industry: a review. Adv Enzym Res 4(2):44–55CrossRefGoogle Scholar
  26. Ja’afaru MI (2013) Screening of fungi isolated from environmental samples for xylanase and cellulase production. ISRN Microbiol 2013:1–7CrossRefGoogle Scholar
  27. Jayaprakasha GK, Jagan Mohan Rao L, Sakariah KK (2005) Chemistry and biological activities of C. longa. Trends Food Sci Technol 16(12):533–548CrossRefGoogle Scholar
  28. Kakule TB, Lin Z, Schmidt EW (2014) Combinatorialization of fungal polyketide synthase–peptide synthetase hybrid proteins. J Am Chem Soc 136(51):17882–17890Google Scholar
  29. Knez Ž, Hrnčič MK, Čolnik M, Škerget M (2017) Chemicals and value added compounds from biomass using sub-and supercritical water. J Supercrit Fluids 133:591–602CrossRefGoogle Scholar
  30. Krishnan A, Convey P, Gonzalez-Rocha G, Alias SA (2016) Production of extracellular hydrolase enzymes by fungi from King George Island. Polar Biol 39:65–76CrossRefGoogle Scholar
  31. Li Y-L, Xin X-M, Chang Z-Y, Shi R-J, Miao Z-M, Ding J, Hao G-P (2015) The endophytic fungi of Salvia miltiorrhiza Bge.f. alba are a potential source of natural antioxidants. Bot Stud 56(1):5PubMedPubMedCentralCrossRefGoogle Scholar
  32. Liao H, Zheng H, Li S, Wei Z, Mei X, Ma H, Xu Y (2015) Functional diversity and properties of multiple xylanases from Penicillium oxalicum GZ-2. Sci Rep 5(1):12631PubMedPubMedCentralCrossRefGoogle Scholar
  33. Lunardelli Negreiros de Carvalho P, de Oliveira Silva E, Aparecida Chagas-Paula D, Honorata Hortolan Luiz J, Ikegaki M (2016) Importance and implications of the production of phenolic secondary metabolites by endophytic fungi: a mini-review. Mini-Rev Med Chem 16:259–271Google Scholar
  34. Ma Y-M, Liang X-A, Kong Y, Jia B (2016) Structural diversity and biological activities of indole diketopiperazine alkaloids from fungi. J Agric Food Chem 64(35):6659–6671PubMedCrossRefGoogle Scholar
  35. Mckelvey MS, Murphy AR (2011) Biotechnological use of fungal enzymes. In: Kavanagh K (ed) Fungi: biology and applications. John Wiley and Sons, Ltd., Chichester, pp 179–202CrossRefGoogle Scholar
  36. Mishra S, Sachan A, Sachan SG (2013) Production of natural value-added compounds: an insight into the eugenol biotransformation pathway. J Ind Microbiol Biotechnol 40(6):545–550PubMedCrossRefGoogle Scholar
  37. Mousa WK, Raizada MN (2013) The diversity of anti-microbial secondary metabolites produced by fungal endophytes: an interdisciplinary perspective. Front Microbiol 4:1–18CrossRefGoogle Scholar
  38. Nigam P (2013) Microbial enzymes with special characteristics for biotechnological applications. Biomol Ther 3(4):597–611Google Scholar
  39. O’Connell JE, Fox PF (2001) Significance and applications of phenolic compounds in the production and quality of milk and dairy products: a review. Int Dairy J 11(3):103–120CrossRefGoogle Scholar
  40. Oliveira LL, Carvalho MV, Melo L (2014) Health promoting and sensory properties of phenolic compounds in food. Rev Ceres 61(suppl):764–779CrossRefGoogle Scholar
  41. Pan F, Su T-J, Cai S-M, Wu W (2017) Fungal endophyte-derived Fritillaria unibracteata var. wabuensis: diversity, antioxidant capacities in vitro and relations to phenolic, flavonoid or saponin compounds. Sci Rep 7(1):42008PubMedPubMedCentralCrossRefGoogle Scholar
  42. Pandey SS, Singh S, Babu CSV, Shanker K, Srivastava NK, Shukla AK, Kalra A (2016) Fungal endophytes of Catharanthus roseus enhance vindoline content by modulating structural and regulatory genes related to terpenoid indole alkaloid biosynthesis. Sci Rep 6(1):26583PubMedPubMedCentralCrossRefGoogle Scholar
  43. Patel K, Gadewar M, Tripathi R, Prasad S, Patel DK (2012) A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”. Asian Pac J Trop Biomed 2(8):660–664PubMedPubMedCentralCrossRefGoogle Scholar
  44. Paulino BN, Pessôa MG, Molina G, Neto AAK, Oliveira JV, Mano MC, Pastore GM (2017) Biotechnological production of value-added compounds by ustilaginomycetous yeasts. Appl Microbiol Biotechnol 101(21):7789–7809PubMedCrossRefGoogle Scholar
  45. Payne CM, Knott BC, Mayes HB, Hansson H, Himmel ME, Sandgren M, Beckham GT (2015) Fungal cellulases. Chem Rev 115(3):1308–1448PubMedCrossRefGoogle Scholar
  46. Pereira MG, Vici AC, Facchini FDA, Tristão AP, Cursino-Santos JR, Sanches PR, Polizeli MDLTDM (2014) Screening of filamentous fungi for lipase production: Hypocrea pseudokoningii, a new producer with a high biotechnological potential. Biocatal Biotransformation 32(1):74–83CrossRefGoogle Scholar
  47. Perviz S, Khan H, Pervaiz A (2016) Plant alkaloids as an emerging therapeutic alternative for the treatment of depression. Front Pharmacol 7:1–7CrossRefGoogle Scholar
  48. Pooja U, Shrivastava R, Agrawal PK (2016) Bioprospecting and biotechnological applications of fungal laccase. 3. Biotechnology 6:15Google Scholar
  49. Qiu M, Xie R, Shi Y, Zhang H, Chen H (2010) Isolation and identification of two flavonoid-producing endophytic fungi from Ginkgo biloba L. Ann Microbiol 60(1):143–150CrossRefGoogle Scholar
  50. Quang TH, Kim DC, Van Kiem P, Van Minh C, Nhiem NX, Tai BH, Oh H (2018) Macrolide and phenolic metabolites from the marine-derived fungus Paraconiothyrium sp. VK-13 with anti-inflammatory activity. J Antibiot (Tokyo) 81:5–9Google Scholar
  51. Ramawat KG, Mérillon JM (2013) Natural products. In: Ramawat KG, Mérillon J-M (eds) Natural products: phytochemistry, botany and metabolism of alkaloids, phenolics and terpenes. Berlin, Heidelberg: SpringerGoogle Scholar
  52. Rana KL, Kour D, Sheikh I, Yadav N, Yadav AN, Kumar V, Singh BP, Dhaliwal HS, Saxena AK (2018a) Biodiversity of endophytic fungi from diverse niches and their biotechnological applications. In: Singh BP (ed) Advances in endophytic fungal research. Springer, Cham. Scholar
  53. Rana KL, Kour D, Yadav AN (2018b) Endophytic microbiomes: biodiversity, ecological significance and biotechnological applications. Res J Biotechnol 14:1–30Google Scholar
  54. Sakthiselvan P, Naveena B, Partha N (2014) Molecular characterization of a xylanase-producing fungus isolated from fouled soil. Braz J Microbiol 45(4):1293–1302PubMedCrossRefGoogle Scholar
  55. Saleem A, Ebrahim MKH (2014) Production of amylase by fungi isolated from legume seeds collected in Almadinah Almunawwarah, Saudi Arabia. J Taibah Univ Sci 8(2):90–97CrossRefGoogle Scholar
  56. Savitha S, Sadhasivam S, Swaminathan K, Lin FH (2011) Fungal protease: production, purification and compatibility with laundry detergents and their wash performance. J Taiwan Inst Chem Eng 42(2):298–304CrossRefGoogle Scholar
  57. Scervino JM, Ponce MA, Erra-Bassells R, Vierheilig H, Ocampo JA, Godeas A (2005) Flavonoids exhibit fungal species and genus specific effects on the presymbiotic growth of Gigaspora and Glomus. Mycol Res 109(7):789–794PubMedCrossRefGoogle Scholar
  58. Shrivastava G, Ownley BH, Augé RM, Toler H, Dee M, Vu A, Chen F (2015) Colonization by arbuscular mycorrhizal and endophytic fungi enhanced terpene production in tomato plants and their defense against a herbivorous insect. Symbiosis 65(2):65–74CrossRefGoogle Scholar
  59. Singh AK, Mukhopadhyay M (2012) Overview of fungal lipase: a review. Appl Biochem Biotechnol 166(2):486–520PubMedCrossRefGoogle Scholar
  60. Singh B, Sharma RA (2015) Plant terpenes: defense responses, phylogenetic analysis, regulation and clinical applications. 3 Biotech 5(2):129–151PubMedCrossRefGoogle Scholar
  61. Singh S, Singh S, Bali V, Sharma L, Mangla J (2014) Production of fungal amylases using cheap, readily available agriresidues, for potential application in textile industry. Biomed Res Int 2014:1–9Google Scholar
  62. Souza PMD, Bittencourt MLDA, Caprara CC, Freitas MD, Almeida RPCD, Silveira D, Magalhães PO (2015) A biotechnology perspective of fungal proteases. Braz J Microbiol 46(2):337–346PubMedPubMedCentralCrossRefGoogle Scholar
  63. Srivastava N, Srivastava M, Mishra PK, Gupta VK, Molina G, Rodriguez-Couto S, Ramteke PW (2018) Applications of fungal cellulases in biofuel production: advances and limitations. Renew Sustain Energy Rev 82(August):2379–2386CrossRefGoogle Scholar
  64. Suman A, Yadav AN, Verma P (2016) Endophytic microbes in crops: diversity and beneficial impact for sustainable agriculture. In: Singh D, Abhilash P, Prabha R (eds) Microbial inoculants in sustainable agricultural productivity: research perspectives. Springer-Verlag, India, pp 117–143. Scholar
  65. Sunitha VH, Ramesha A, Savitha J, Srinivas C (2012) Amylase production by endophytic fungi Cylindrocephalum sp. isolated from medicinal plant Alpinia calcarata (Haw.) Roscoe. Braz J Microbiol 43(3):1213–1221PubMedPubMedCentralCrossRefGoogle Scholar
  66. Tabatabaei M, Karimi K, Sárvári Horváth I, Kumar R (2015) Recent trends in biodiesel production. Biofuel Res J 2(3):258–267CrossRefGoogle Scholar
  67. Venkata Subhash G, Venkata Mohan S (2011) Biodiesel production from isolated oleaginous fungi Aspergillus sp. using corncob waste liquor as a substrate. Bioresour Technol 102(19):9286–9290PubMedCrossRefGoogle Scholar
  68. Vicente G, Bautista LF, Rodríguez R, Gutiérrez FJ, Sádaba I, Ruiz-Vázquez RM, Garre V (2009) Biodiesel production from biomass of an oleaginous fungus. Biochem Eng J 48(1):22–27CrossRefGoogle Scholar
  69. Wang Y, Gao BL, Li XX, Zhang ZB, Yan RM, Yang HL, Zhu D (2015) Phylogenetic diversity of culturable endophytic fungi in Dongxiang wild rice (Oryza rufipogon Griff), detection of polyketide synthase gene and their antagonistic activity analysis. Fungal Biol 119(11):1032–1045PubMedCrossRefGoogle Scholar
  70. Wu C, Zhao Y, Chen R, Liu D, Liu M, Proksch P, Lin W (2016a) Phenolic metabolites from mangrove-associated Penicillium pinophilum fungus with lipid-lowering effects. RSC Adv 6(26):21969–21978CrossRefGoogle Scholar
  71. Wu W, Tran W, Taatjes CA, Alonso-Gutierrez J, Lee TS, Gladden JM (2016b) Rapid discovery and functional characterization of terpene synthases from four endophytic Xylariaceae. PLoS One 11(2):e0146983PubMedPubMedCentralCrossRefGoogle Scholar
  72. Xu W, Gavia DJ, Tang Y (2014) Biosynthesis of fungal indole alkaloids. Nat Prod Rep 31(10):1474–1487PubMedPubMedCentralCrossRefGoogle Scholar
  73. Yadav AN, Sachan SG, Verma P, Kaushik R, Saxena AK (2016) Cold active hydrolytic enzymes production by psychotrophic bacilli isolated from three sub-glacial lakes of NW Indian Himalayas. J Basic Microbiol 56:294–307PubMedCrossRefGoogle Scholar
  74. Yadav AN, Kumar R, Kumar S, Kumar V, Sugitha T, Singh B, Chauhan VS, Dhaliwal HS, Saxena AK (2017a) Beneficial microbiomes: biodiversity and potential biotechnological applications for sustainable agriculture and human health. J Appl Biol Biotechnol 5:1–13CrossRefGoogle Scholar
  75. Yadav AN, Verma P, Sachan SG, Saxena AK (2017b) Biodiversity and biotechnological applications of psychrotrophic microbes isolated from Indian Himalayan regions. EC Microbiol ECO 1:48–54Google Scholar
  76. Yan C, Liu W, Li J, Deng Y, Chen S, Liu H (2018) Bioactive terpenoids from Santalum album derived endophytic fungus Fusarium sp. YD-2. RSC Adv 8(27):14823–14828CrossRefGoogle Scholar
  77. Yike I (2011) Fungal proteases and their pathophysiological effects. Mycopathologia 171(5):299–323PubMedCrossRefGoogle Scholar
  78. Yopi, Tasia W, Melliawati R (2017) Cellulase and xylanase production from three isolates of indigenous endophytic fungi. IOP Conf Ser Earth Environ Sci 101(1):012035CrossRefGoogle Scholar
  79. Yu H, Zhang L, Li L, Zheng C, Guo L, Li W, Qin L (2010) Recent developments and future prospects of antimicrobial metabolites produced by endophytes. Microbiol Res 165(6):437–449PubMedCrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Paramanantham Parasuraman
    • 1
  • Busi Siddhardha
    • 1
  1. 1.Department of Microbiology, School of Life SciencesPondicherry UniversityPuducherryIndia

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