Sharp Images Detection for Microscope Pollen Slides Observation
In this paper, a new preprocessing algorithm to qualify images of different pollen grains for further processing is proposed. This algorithm provides a score related to the sharpness of the image and will be used to automatically adjust the focal length of a microscope that magnifies the image. The obtained score has been compared to four quality metrics generally used to estimate the clarity of an image and to a reference made by a human. The results of the simulations show that the proposed algorithm combines better performance with low complexity on the set of images.
KeywordsMicroscope slide image acquisition Sharp image detection Fourier transform
The authors would like to gratefully acknowledge the support of the National Research Agency and the STAE foundation under the auspices of the Saint-Exupery Technological Research Institute without which the present study could not have been completed.
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