Pomegranate (Punica granatum) Seed Oil

  • Sarfaraz Ahmed Mahesar
  • Abdul Hameed Kori
  • Syed Tufail Hussain Sherazi
  • Aftab Ahmed Kandhro
  • Zahid Husain Laghari


Pomegranate is an earliest and holy fruit affectionately known as the “jewel of winter” belongs to the Punicaceae family. Throughout the world, ~500 known pomegranate varieties available which reveal different quality characteristics of fruit such as size, shape, color, flavor and taste and seed hardness. The pomegranate seeds contain approximately 3% of total fruit weight, which contains typically oil in the range of 12–20%. Conjugated fatty acids are present in many plant oils with varying concentrations including pomegranate seed oil. Conjugated fatty acids are the geometric and positional isomers of polyunsaturated fatty acids with alternate double bonds. These fatty acids received remarkable interest due to valuable physiological effects on various diseases. The pomegranate seed oil contains higher concentration (>70%) of conjugated fatty acids in the form of punicic acid (9cis, 11trans, 13cis-conjugated linolenic acid). In the present chapter, chemistry and functionality of pomegranate fruit and seed oil especially conjugated fatty acids are reviewed.


Conjugated fatty acids Ellagic acid Tocols Seed oil Conjugated fatty acid Punicic acid 



Conjugated linoleic acid


Conjugated linolenic acid

E. coli

Escherichia coli


Ellagic acid


Erectile dysfunction




Low density lipid


Nitric oxide


Punicic acid


Pomegranate flower leaf extract


Pomegranate juice


Peroxisome proliferator-activated receptor




Ellagic acid


  1. Abbasi, H., Rezaei, K., & Rashidi, L. (2008). Extraction of essential oils from the seeds of pomegranate using organic solvents and supercritical CO2. Journal of the American Oil Chemists’ Society, 85, 83–89.CrossRefGoogle Scholar
  2. Al-Maiman, S. A., & Ahmad, D. (2002). Changes in physical and chemical properties during pomegranate (Punica granatum L.) fruit maturation. Food Chemistry, 76, 437–441.CrossRefGoogle Scholar
  3. Amakura, Y., Okada, M., Tsuji, S., et al. (2000). High-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits. Journal of Chromatography A, 896, 87–93.CrossRefGoogle Scholar
  4. Amri, Z., Lazreg-Aref, H., Mekni, M., et al. (2017a). Oil characterization and lipids class composition of pomegranate seeds. Biomed Research International, 2037341, 8 pp. Scholar
  5. Amri, Z., Zaouay, F., Lazreg-Aref, H., et al. (2017b). Phytochemical content, fatty acids composition and antioxidant potential of different pomegranate parts: Comparison between edible and non edible varieties grown in Tunisia. International Journal of Biological Macromolecules, 104, 274–280.CrossRefGoogle Scholar
  6. Aviram, M., Dornfeld, L., Rosenblat, M., et al. (2000). Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: Studies in humans and in atherosclerotic apolipoprotein E-deficient mice. The American Journal of Clinical Nutrition, 71, 1062–1076.CrossRefGoogle Scholar
  7. Aviram, M., Volkova, N., Coleman, R., et al. (2008). Pomegranate phenolics from the peels, arils, and flowers are antiatherogenic: Studies in vivo in atherosclerotic apolipoprotein e-deficient (E 0) mice and in vitro in cultured macrophages and lipoproteins. Journal of Agricultural and Food Chemistry, 56(3), 1148–1157.CrossRefGoogle Scholar
  8. Banihani, S., Swedan, S., & Alguraan, Z. (2013). Pomegranate and type 2 diabetes. Nutrition Research, 33, 341–348.CrossRefGoogle Scholar
  9. Barzegar, M., Fadavi, A., & Azizi, M. (2004). An investigation on the physico-chemical composition of various pomegranates (Punica granatum L.) grown in Yazd. Iranian. Journal of Food Science and Technology, 1, 9–14.Google Scholar
  10. Bopitiya, D., & Madhujith, T. (2012). Antioxidant potential of pomegranate (Punica granatum L.) cultivars grown in Sri Lanka. Tropical Agricultural Research, 24, 71–81.CrossRefGoogle Scholar
  11. Bouroshaki, M. T., Sadeghnia, H. R., Banihasan, M., et al. (2010). Protective effect of pomegranate seed oil on hexachlorobutadiene-induced nephrotoxicity in rat kidneys. Renal Failure, 32, 612–617.CrossRefGoogle Scholar
  12. Boussetta, T., Raad, H., Lettéron, P., et al. (2009). Punicic acid a conjugated linolenic acid inhibits TNFα-induced neutrophil hyperactivation and protects from experimental colon inflammation in rats. PLoS One, 4, e6458.CrossRefGoogle Scholar
  13. Çam, M., Hışıl, Y., & Durmaz, G. (2009). Classification of eight pomegranate juices based on antioxidant capacity measured by four methods. Food Chemistry, 112, 721–726.CrossRefGoogle Scholar
  14. Celik, I., Temur, A., & Isik, I. (2009). Hepatoprotective role and antioxidant capacity of pomegranate (Punica granatum) flowers infusion against trichloroacetic acid-exposed in rats. Food and Chemical Toxicology, 47, 145–149.CrossRefGoogle Scholar
  15. Dadashi, S., Mousazadeh, M., Emam-Djomeh, Z., et al. (2013). Pomegranate (Punica granatum L.) seed: A comparative study on biochemical composition and oil physicochemical characteristics. International Journal of Advanced Biological and Biomedical Research, 1, 351–363.Google Scholar
  16. Davidson, M. H., Maki, K. C., Dicklin, M. R., et al. (2009). Effects of consumption of pomegranate juice on carotid intima-media thickness in men and women at moderate risk for coronary heart disease. The American Journal of Cardiology, 104, 936–942.CrossRefGoogle Scholar
  17. Eikani, M. H., Golmohammad, F., & Homami, S. S. (2012). Extraction of pomegranate (Punica granatum L.) seed oil using superheated hexane. Food and Bioproducts Processing, 90, 32–36.CrossRefGoogle Scholar
  18. Elfalleh, W., Tlili, N., Nasri, N., et al. (2011). Antioxidant capacities of phenolic compounds and tocopherols from tunisian pomegranate (Punica granatum) fruits. Journal of Food Science, 76(5), C707–C713.CrossRefGoogle Scholar
  19. El-Nemr, S., Ismail, I., & Ragab, M. (1990). Chemical composition of juice and seeds of pomegranate fruit. Molecular Nutrition & Food Research, 34, 601–606.Google Scholar
  20. Ercisli, S., Agar, G., Orhan, E., et al. (2007). Interspecific variability of RAPD and fatty acid composition of some pomegranate cultivars (Punica granatum L.) growing in southern Anatolia region in Turkey. Biochemical Systematics and Ecology, 35, 764–769.CrossRefGoogle Scholar
  21. Fadavi, A., Barzegar, M., Azizi, M., et al. (2005). Note. Physicochemical composition of ten pomegranate cultivars (Punica granatum L.) grown in Iran. Food Science and Technology International, 11, 113–119.CrossRefGoogle Scholar
  22. Fadavi, A., Barzegar, M., & Azizi, M. H. (2006). Determination of fatty acids and total lipid content in oilseed of 25 pomegranates varieties grown in Iran. Journal of Food Composition and Analysis, 19, 676–680.CrossRefGoogle Scholar
  23. Galaverna, G., Di Silvestro, G., Cassano, A., et al. (2008). A new integrated membrane process for the production of concentrated blood orange juice: Effect on bioactive compounds and antioxidant activity. Food Chemistry, 106, 1021–1030.CrossRefGoogle Scholar
  24. Gil, M. I., Tomás-Barberán, F. A., Hess-Pierce, B., et al. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48, 4581–4589.CrossRefGoogle Scholar
  25. Grossmann, M. E., Mizuno, N. K., Schuster, T., et al. (2010). Punicic acid is an ω-5 fatty acid capable of inhibiting breast cancer proliferation. International Journal of Oncology, 36, 421–426.PubMedGoogle Scholar
  26. Habibnia, M., Ghavami, M., Ansaripour, M., et al. (2012). Chemical evaluation of oils extracted from five different varieties of Iranian pomegranate seeds. Journal of Food Biosciences and Technology, 2, 35–40.Google Scholar
  27. Heber, D., Seeram, N. P., Wyatt, H., et al. (2007). Safety and antioxidant activity of a pomegranate ellagitannin enriched polyphenol dietary supplement in overweight individuals with increased waist size. Journal of Agricultural and Food Chemistry, 55(24), 10050–10054.CrossRefGoogle Scholar
  28. Hiwale, S. S. (2009). The pomegranate. New Delhi: New India Publishing Agency.Google Scholar
  29. Holland, D., Larkov, O., & Bar Ya akov, I. (2008). The pomegranate: New interest in an ancient fruit. Chronicle Horticulture, 48, 12–15.Google Scholar
  30. Holland, D., Hatib, K., & Bar-Ya’akov, I. (2009). Pomegranate: Botany, horticulture, breeding. Horticultural Reviews, 35, 127–191.CrossRefGoogle Scholar
  31. Ignarro, L. J., Byrns, R. E., Sumi, D., et al. (2006). Pomegranate juice protects nitric oxide against oxidative destruction and enhances the biological actions of nitric oxide. Nitric Oxide, 15, 93–102.CrossRefGoogle Scholar
  32. Jahfar, M., Vijayan, K., & Azadi, P. (2003). Studies on a polysaccharide from the fruit rind of Punica granatum. Research Journal of Chemistry and Environment, 7, 43–50.Google Scholar
  33. Jaiswal, V., DerMarderosian, A., & Porter, J. R. (2010). Anthocyanins and polyphenol oxidase from dried arils of pomegranate (Punica granatum L.). Food Chemistry, 118, 11–16.CrossRefGoogle Scholar
  34. Kaufman, M., & Wiesman, Z. (2007). Pomegranate oil analysis with emphasis on MALDI-TOF/MS triacylglycerol fingerprinting. Journal of Agricultural and Food Chemistry, 55, 10405–10413.CrossRefGoogle Scholar
  35. Kaur, G., Jabbar, Z., Athar, M., et al. (2006). Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food and Chemical Toxicology, 44, 984–993.CrossRefGoogle Scholar
  36. Kohno, H., Suzuki, R., Yasui, Y., et al. (2004). Pomegranate seed oil rich in conjugated linolenic acid suppresses chemically induced colon carcinogenesis in rats. Cancer Science, 95, 481–486.CrossRefGoogle Scholar
  37. Laghari, Z. H., Mahesar, S. A., Sherazi, S. T. H., Memon, S. A., Sirajuddin, Mugheri, G. A., Shah, S. N., Panhwar, T., & Chang, A. S. (2018). Quality evaluation of pomegranate waste and extracted oil. International Food Research Journal, 25(3), 1295–1299.Google Scholar
  38. Lan, J. Q., Lei, F., Hua, L., et al. (2009). Transport behavior of ellagic acid of pomegranate leaf tannins and its correlation with total cholesterol alteration in HepG2 cells. Biomedical Chromatography, 23, 531–536.CrossRefGoogle Scholar
  39. Lansky, E. P., & Newman, R. A. (2007). Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of Ethnopharmacology, 109, 177–206.CrossRefGoogle Scholar
  40. Lansky, E. P., Harrison, G., Froom, P., et al. (2005). Pomegranate (Punica granatum) pure chemicals show possible synergistic inhibition of human PC-3 prostate cancer cell invasion across Matrigel™. Investigational New Drugs, 23, 121–122.CrossRefGoogle Scholar
  41. Li, Y., Guo, C., Yang, J., et al. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry, 96, 254–260.CrossRefGoogle Scholar
  42. Mackler, A. M., Heber, D., & Cooper, E. L. (2013). Pomegranate: Its health and biomedical potential. Evidence-Based Complementary and Alternative Medicine, 903457, 2 pp. Scholar
  43. Marwat, S. K., Khan, M. A., Khan, M. A., et al. (2009). Fruit plant species mentioned in the Holy Qura’n and Ahadith and their ethnomedicinal importance. American-Eurasian Journal of Agricultural & Environmental Sciences, 5, 284–295.Google Scholar
  44. Melgarejo, P., & Artes, F. (2000). Total lipid content and fatty acid composition of oilseed from lesser known sweet pomegranate clones. Journal of the Science of Food and Agriculture, 80, 1452–1454.CrossRefGoogle Scholar
  45. Melo, I. L.P., Carvalho, E. B. T., & Filho, J. M. (2014). Pomegranate seed oil (Punica granatum L.): A source of punicic acid (conjugated α-linolenic acid). Journal of Human Nutrition & Food Science, 2(1), 1-11.Google Scholar
  46. Melo, I. L.P., Carvalho, E. B. T. D., Silva, A. M. O., et al. (2016). Characterization of constituents, quality and stability of pomegranate seed oil (Punica granatum L.). Food Science and Technology, 36, 132–139.CrossRefGoogle Scholar
  47. Mena, P., Vegara, S., Martí, N., et al. (2013). Changes on indigenous microbiota, colour, bioactive compounds and antioxidant activity of pasteurised pomegranate juice. Food Chemistry, 141, 2122–2129.CrossRefGoogle Scholar
  48. Mirdehghan, S. H., & Rahemi, M. (2007). Seasonal changes of mineral nutrients and phenolics in pomegranate (Punica granatum L.) fruit. Scientia Horticulturae, 111, 120–127.CrossRefGoogle Scholar
  49. Mousavinejad, G., Emam-Djomed, Z., Rezaei, K., et al. (2009). Identification and quantification ofphenolic compounds and their effects on antioxidant activity in pomegranate juices of eight Iranian cultivars. Food Chemistry, 115, 1274–1278.CrossRefGoogle Scholar
  50. Mukherjee, C., & Bhattacharyya, D. (2006). Oxidative stability of some seed oils containing conjugated octadecatrienoic fatty acids isomers. Journal of Lipid Science and Technology, 32, 225–227.Google Scholar
  51. Neuhofer, H., Witte, L., Gorunovic, M., et al. (1993). Alkaloids in the bark of Punica granatum L. (pomegranate) from Yugoslavia. Pharmazie, 48, 389–391.Google Scholar
  52. Ozgen, M., Durgaç, C., Serçe, S., & Kaya, C. (2008). Chemical and antioxidant properties of pomegranate cultivars grown in the Mediterranean region of Turkey. Food Chemistry, 111(3), 703–706.CrossRefGoogle Scholar
  53. Özgül-Yücel, S. (2005). Determination of conjugated linolenic acid content of selected oil seeds grown in Turkey. Journal of the American Oil Chemists’ Society, 82, 893–897.CrossRefGoogle Scholar
  54. Pande, G., & Akoh, C. C. (2009). Antioxidant capacity and lipid characterization of six Georgia grown pomegranate cultivers. Journal of Agricultural and Food Chemistry, 57, 9427–9436.CrossRefGoogle Scholar
  55. Parashar, A. (2010). Lipid content and fatty acid composition of seed oils from six pomegranate cultivars. International Journal of Fruit Science, 10(4), 425–430.CrossRefGoogle Scholar
  56. Parashar, A., Gupta, C., Gupta, S., et al. (2009). Antimicrobial ellagitannin from pomegranate (Punica granatum) fruits. International Journal of Fruit Science, 9, 226–231.CrossRefGoogle Scholar
  57. Poyrazoğlu, E., Gökmen, V., & Artιk, N. (2002). Organic acids and phenolic compounds in pomegranates (Punica granatum L.) grown in Turkey. Journal of Food Composition and Analysis, 15, 567–575.CrossRefGoogle Scholar
  58. Prashantha, M. A. B., Premachandra, J. K., & Amarasinghe, A. D. U. S. (2009). Composition, physical properties and drying characteristics of seed oil of Momordica charantia cultivated in Sri Lank. Journal of the American Oil Chemists’ Society, 86, 27–32.CrossRefGoogle Scholar
  59. Saha, S., & Ghosh, M. (2009). Comparative study of antioxidant activity of α-eleostearic acid and punicic acid against oxidative stress generated by sodium arsenite. Food and Chemical Toxicology, 47, 2551–2556.CrossRefGoogle Scholar
  60. Sassano, G., Sanderson, P., Franx, J., et al. (2009). Analysis of pomegranate seed oil for the presence of jacaric acid. Journal of the Science of Food and Agriculture, 89, 1046–1052.CrossRefGoogle Scholar
  61. Seeram, N. P., Schulman, R. N., & Heber, D. (2006). Pomegranates: Ancient roots to modern medicine. Boca Raton: CRC/Taylor and Francis.Google Scholar
  62. Sinha, S., Thakur, D. S., Mishra, P. K., et al. (2016). D2-analysis suggests wider genetic divergence in pomegranate genotypes. The Bioscan, 11(2), 1011–1015.Google Scholar
  63. Soetjipto, H., Pradipta, M., & Timotius, K. (2010). Fatty acids composition of red and purple pomegranate (Punica granatum L) seed oil. Journal of Cancer Chemoprevention, 1, 74–77.Google Scholar
  64. Spilmont, M., Léotoing, L., Davicco, M.-J., et al. (2013). Pomegranate seed oil prevents bone loss in a mice model of osteoporosis, through osteoblastic stimulation, osteoclastic inhibition and decreased inflammatory status. The Journal of Nutritional Biochemistry, 24, 1840–1848.CrossRefGoogle Scholar
  65. Suzuki, R., Noguchi, R., Ota, T., Abe, M., Miyashita, K., & Kawada, T. (2001). Cytotoxic effect of conjugated trienoic fatty acids on mouse tumor and human monocytic leukemia cells. Lipids, 36(5), 477–482.CrossRefGoogle Scholar
  66. Syed, D. N., Afaq, F., & Mukhtar, H. (2007). Pomegranate derived products for cancer chemoprevention. Seminars in Cancer Biology, 17(5), 377–385.CrossRefGoogle Scholar
  67. Tabaraki, R., Heidarizadi, E., & Benvidi, A. (2012). Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) peel antioxidants by response surface methodology. Separation and Purification Technology, 98, 16–23.CrossRefGoogle Scholar
  68. Tanaka, T., Hosokawa, M., Yasui, Y., et al. (2011). Cancer chemopreventive ability of conjugated linolenic acids. International Journal of Molecular Sciences, 12(11), 7495–7509.CrossRefGoogle Scholar
  69. Tezcan, F., Gültekin-Özgüven, M., Diken, T., et al. (2009). Antioxidant activity and total phenolic, organic acid and sugar content in commercial pomegranate juices. Food Chemistry, 115, 873–877.CrossRefGoogle Scholar
  70. Van Elswijk, D. A., Schobel, U. P., Lansky, et al. (2004). Rapid dereplication of estrogenic compounds in pomegranate (Punica grantum) using on-line biochemical detection coupled to mass spectrometry. Phytochemistry, 65, 233–241.CrossRefGoogle Scholar
  71. Verardo, V., Garcia-Salas, P., Baldi, E., et al. (2014). Pomegranate seeds as a source of nutraceutical oil naturally rich in bioactive lipids. Food Research International, 65, 445–452.CrossRefGoogle Scholar
  72. Viuda-Martos, M., Fernández-López, J., & Pérez-Álvarez, J. (2010). Pomegranate and its many functional components as related to human health: A review. Comprehensive Reviews in Food Science and Food Safety, 9, 635–654.CrossRefGoogle Scholar
  73. Wang, L., & Martins-Green, M. (2014). Pomegranate and its components as alternative treatment for prostate cancer. International Journal of Molecular Sciences, 15, 14949–14966.CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Sarfaraz Ahmed Mahesar
    • 1
  • Abdul Hameed Kori
    • 1
  • Syed Tufail Hussain Sherazi
    • 1
  • Aftab Ahmed Kandhro
    • 2
  • Zahid Husain Laghari
    • 1
  1. 1.National Centre of Excellence in Analytical ChemistryUniversity of SindhJamshoroPakistan
  2. 2.Dr. M.A. Kazi Institute of ChemistryUniversity of SindhJamshoroPakistan

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