High-Throughput Fluorescence Assays for Ion Channels and GPCRs

  • Irina VetterEmail author
  • David Carter
  • John Bassett
  • Jennifer R. Deuis
  • Bryan Tay
  • Sina Jami
  • Samuel D. Robinson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1131)


Ca2+, Na+ and K+- permeable ion channels as well as GPCRs linked to Ca2+ release are important drug targets. Accordingly, high-throughput fluorescence plate reader assays have contributed substantially to drug discovery efforts and pharmacological characterization of these receptors and ion channels. This chapter describes some of the basic properties of the fluorescent dyes facilitating these assay approaches as well as general methods for establishment and optimisation of fluorescence assays for ion channels and Gq-coupled GPCRs.


High-throughput High-content Fluorescence imaging G protein-coupled receptor Voltage-gated ion channel Ligand-gated ion channel Assay development Optimization FLIPR 



adenosine triphosphate


calcium ion

CaV and VGCC

Voltage-gated Ca2+ channels




Fluorescent Imaging Plate Reader


G-protein coupled receptor


high throughput screening




Ligand-gated Ca2+ channels


Na+/Ca2+ exchanger


phosphatidylinositol 4, 5 bisphosphate


Plasma Membrane Ca2+ ATPase


ryanodine receptors


sarco/endoplasmic reticulum Ca2+ ATPase


ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid.


2-aminophenol-N,N,O-triacetic acid


1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid


dissociation constant




endoplasmic reticulum


light-emitting diode


charge-coupled device


Electron Multiplying Charge Coupled Device


Intensified CCD








nicotinic acetylcholine receptors


4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid


protease-activated receptor 2


relative fluorescence unit


Sodium-binding benzofuran isophthalate


Potassium-binding benzofuran isophthalate


Dorsal Root Ganglion


Regulator of G protein signalling 4


voltage-gated sodium channel


mitochondrial Ca2+ uniporter


cyclic adenosine monophosphate


Cyclic AMP iNdirect Detection by Light Emission from Sensor cells


guanosine triphosphate


Transient Receptor Potential


small-conductance calcium-activated K+ channel


intermediate-conductance calcium-activated K+ channel


big-conductance calcium-activated K+ channel


Inwardly-rectifying K+ channel


Tandem of pore domains in a Weakly Inward rectifying K+ channel


TWIK-related K+ channel


TWIK-related acid-sensitive K+ channel


TWIK-related alkaline pH-activated K+ channel


TWIK-related halothane-inhibited K+ channel


TWIK-related spinal cord K+ channel


voltage-gated K+ channel

ANG-1 and ANG-2

Asante NaTRIUM Green-1 and -2


Pyridinium, 4-(2-(6(dibutylamino)-2-naphthalenyl)-1-(3-sulfopropyl)-hydroxide


bis-(1,3-dibutylbarbituric acid) trimethine oxonol


FLIPR Membrane Potential


fluorescence resonance energy transfer


photoinduced electron transfer


N-[6-chloro-7-hydroxycourmarin-3-carbonyl] dimyristroyl phosphatidyl ethanolamine


genetically encoded voltage indicators






light-emitting diode


scientific complementary metal-oxide-semiconductor


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Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Irina Vetter
    • 1
    • 2
    Email author
  • David Carter
    • 1
  • John Bassett
    • 2
  • Jennifer R. Deuis
    • 1
  • Bryan Tay
    • 1
  • Sina Jami
    • 1
  • Samuel D. Robinson
    • 1
  1. 1.Institute for Molecular BioscienceThe University of QueenslandSt. LuciaAustralia
  2. 2.School of PharmacyThe University of QueenslandSt. LuciaAustralia

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