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Proteomics in Vascular Biology

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Fundamentals of Vascular Biology

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Abstract

The proteome is the entire set of proteins of a biological sample, and proteomics is the large-scale qualitative (protein composition) and quantitative analysis of the proteome. A collection of dedicated biochemical methods is combined with protein databases to identify the proteins and to allocate them to biological pathways. The work process involves two major steps, the separation of the proteins (electrophoresis and liquid chromatography) and the protein identification by mass spectrometry. In medical research, proteomics is mainly applied to discover proteins that play a role in pathological processes; thus it is also a valuable tool in vascular biology. These disease-related proteins are generally defined as biomarkers and are of great interest to the diagnosis and treatment of patients. In vascular research, biological samples are, for example, plasma, serum, platelets, endothelial cells and vascular smooth muscle cells. This chapter will provide an overview of this technology and will demonstrate its application.

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Authors and Affiliations

  1. Department of Vascular Biology and Thrombosis Research, Center for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria

    Maria Zellner & Ellen Umlauf

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  1. Maria Zellner
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Correspondence to Maria Zellner .

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  1. Department of Vascular Biology and Thrombosis Research, Center for Physiology and Pharmacology, Medical University of Vienna, Wien, Austria

    Margarethe Geiger

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Zellner, M., Umlauf, E. (2019). Proteomics in Vascular Biology. In: Geiger, M. (eds) Fundamentals of Vascular Biology. Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-030-12270-6_17

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