Advertisement

Transcriptional Regulation of Gene Expression Related to Hydrogen Peroxide (H2O2) and Nitric Oxide (NO)

  • Juan C. Begara-Morales
  • Mounira Chaki
  • Raquel Valderrama
  • Capilla Mata-Pérez
  • María Padilla
  • Juan B. BarrosoEmail author
Chapter

Abstract

Hydrogen peroxide (H2O2) and nitric oxide (NO) are biological messengers that control a plethora of physiological functions integral to plant biology such as seed germination, growth, development, flowering, or plant response to stress. Furthermore, the interplay between the signaling pathways governed by these redox molecules has emerged as crucial during plant response to different stress situations. In recent years, to gain in the knowledge of the mode of action of these signaling molecules at molecular levels, different NO donors and H2O2 have been used in medium- and large-scale transcriptomic analyses including microarray, cDNA-amplification fragment length polymorphism (AFLP), and high-throughput sequencing (RNA-seq technology). Following this strategy, a high transcriptional reprogramming induced by both NO and H2O2 has been proposed. In this regard, thousands of NO- and H2O2-cell targets have been identified in different plant species and organs and predicted to be related to a wide diversity of biological processes. However, some authors have identified by comparing different transcriptomic analysis that there is a low overlap in the transcriptomic data available under different treatment conditions as well as different organ analyzed. In this sense, more transcriptomic data comparisons will help in the identification of the NO- and H2O2-specific targets and even the common genes involved in both H2O2- and NO-dependent signaling events.

In this book chapter, we will offer an update about the recent knowledge concerning the transcriptional regulation induced by NO and H2O2. With this purpose, the recent data from the different medium- and large-scale transcriptomic analyses have been discussed. In addition, it is also provided an overview about the interplay between H2O2- and NO-dependent signaling mechanism and the need to further identification of common targets during the coordinated response to different stress situations.

Keywords

Nitric oxide Hydrogen peroxide Signaling Transcriptomic analysis RNA-seq Microarray cDNA-AFLP 

Notes

Acknowledgments

JCBM wishes to thank the Ministry of Economy and Competitiveness (Spain) for postdoctoral research funding within the Juan de la Cierva-Incorporación program. The work in our lab is supported by the ERDF grants co-financed by the Ministry of Economy and Competitiveness (projects BIO2015-66390-P) and the Junta de Andalucía (group BIO286) in Spain.

References

  1. Ahlfors R, Brosché M, Kollist H, Kangasjarvi J (2009) Nitric oxide modulates ozone-induced cell death, hormone biosynthesis and gene expression in Arabidopsis thaliana. Plant J 58:1–12CrossRefGoogle Scholar
  2. Albertos P, Romero-Puertas MC, Tatematsu K, Mateos I, Sánchez-Vicente I, Nambara E, Lorenzo O (2015) S-nitrosylation triggers ABI5 degradation to promote seed germination and seedling growth. Nat Commun 6:8669CrossRefGoogle Scholar
  3. Andrio E, Marino D, Marmeys A, de Segonzac MD, Damiani I, Genre A, Huguet S, Frendo P, Puppo A, Pauly N (2013) Hydrogen peroxide-regulated genes in the Medicago truncatula-Sinorhizobium meliloti symbiosis. New Phytol 198:179–189CrossRefGoogle Scholar
  4. Astier J, Lindermayr C (2012) Nitric oxide-dependent posttranslational modification in plants: an update. Int J Mol Sci 13:15193–15208CrossRefGoogle Scholar
  5. Badri DV, Loyola-Vargas VM, Du J, Stermitz FR, Broeckling CD, Iglesias-Andreu L, Vivanco JM (2008) Transcriptome analysis of Arabidopsis roots treated with signaling compounds: a focus on signal transduction, metabolic regulation and secretion. New Phytol 179:209–223CrossRefGoogle Scholar
  6. Begara-Morales JC, Loake GJ (2016) Protein denitrosylation in plant biology. In: Lamattina L, García-Mata C (eds) Gasotransmitters in plants, signaling and communication in plants. Springer, Cham, pp 201–215CrossRefGoogle Scholar
  7. Begara-Morales JC, Sánchez-Calvo B, Chaki M, Valderrama R, Mata-Pérez C, López-Jaramillo J, Padilla MN, Carreras A, Corpas FJ, Barroso JB (2014a) Dual regulation of cytosolic ascorbate peroxidase (APX) by tyrosine nitration and S-nitrosylation. J Exp Bot 65:527–538CrossRefGoogle Scholar
  8. Begara-Morales JC, Sánchez-Calvo B, Luque F, Leyva-Pérez MO, Leterrier M, Corpas FJ, Barroso JB (2014b) Differential transcriptomic analysis by RNA-seq of GSNO-responsive genes between Arabidopsis roots and leaves. Plant Cell Physiol 55:1080–1095CrossRefGoogle Scholar
  9. Begara-Morales JC, Sánchez-Calvo B, Chaki M, Valderrama R, Mata-Pérez C, Padilla MN, Corpas FJ, Barroso JB (2016) Antioxidant systems are regulated by nitric oxide-mediated post-translational modifications (NO-PTMs). Front Plant Sci 7:152CrossRefGoogle Scholar
  10. Begara-Morales JC, Chaki M, Valderrama R, Sanchez-Calvo B, Mata-Perez C, Padilla MN, Corpas FJ, Barroso JB (2018) Nitric oxide buffering and conditional nitric oxide release in stress response. J Exp Bot 69:3425–3438CrossRefGoogle Scholar
  11. Besson-Bard A, Gravot A, Richaud P, Auroy P, Duc C, Gaymard F, Taconnat L, Renou JP, Pugin A, Wendehenne D (2009a) Nitric oxide contributes to cadmium toxicity in Arabidopsis by promoting cadmium accumulation in roots and by up-regulating genes related to iron uptake. Plant Physiol 149:1302–1315CrossRefGoogle Scholar
  12. Besson-Bard AL, Astier JR, Rasul S, Wawer I, Dubreuil-Maurizi C, Jeandroz S, Wendehenne D (2009b) Current view of nitric oxide-responsive genes in plants. Plant Sci 177:302–309CrossRefGoogle Scholar
  13. Blaby IK, Blaby-Haas CE, Pérez-Pérez ME, Schmollinger S, Fitz-Gibbon S, Lemaire SD, Merchant SS (2015) Genome-wide analysis on Chlamydomonas reinhardtii reveals the impact of hydrogen peroxide on protein stress responses and overlap with other stress transcriptomes. Plant J 84:974–988CrossRefGoogle Scholar
  14. Boscari A, del Giudice J, Ferrarini A, Venturini L, Zaffini A-L, Delledonne M, Puppo A (2013) Expression dynamics of the Medicago truncatula transcriptome during the symbiotic interaction with Sinorhizobium meliloti: which role for nitric oxide? Plant Physiol 161:425–439CrossRefGoogle Scholar
  15. Camejo D, Ortiz-Espín A, Lázaro JJ, Romero-Puertas MC, Lázaro-Payo A, Sevilla F, Jiménez A (2015) Functional and structural changes in plant mitochondrial PrxII F caused by NO. J Proteome 119:112–125CrossRefGoogle Scholar
  16. Cerny M, Habánová H, Berka M, Luklová M, Brzobohatý B (2018) Hydrogen peroxide: its role in plant biology and crosstalk with signalling networks. Int J Mol Sci 19:2812CrossRefGoogle Scholar
  17. Corpas FJ, Barroso JB (2013) Nitro-oxidative stress vs oxidative or nitrosative stress in higher plants. New Phytol 199:633–635CrossRefGoogle Scholar
  18. Corpas FJ, Leterrier M, Valderrama R, Airaki M, Chaki M, Palma JM, Barroso JB (2011) Nitric oxide imbalance provokes a nitrosative response in plants under abiotic stress. Plant Sci 181:604–611CrossRefGoogle Scholar
  19. Corpas FJ, Alché JD, Barroso JB (2013) Current overview of S-nitrosoglutathione (GSNO) in higher plants. Front Plant Sci 4:126PubMedPubMedCentralGoogle Scholar
  20. Damiani I, Pauly N, Puppo A, Brouquisse R, Boscari A (2016) Reactive oxygen species and nitric oxide control early steps of the legume-Rhizobium symbiotic interaction. Front Plant Sci 7:454PubMedPubMedCentralGoogle Scholar
  21. De Michele R, Formentin E, Todesco M, Toppo S, Carimi F, Zottini M, Barizza E, Ferrarini A, Delledonne M, Fontana P (2009) Transcriptome analysis of Medicago truncatula leaf senescence: similarities and differences in metabolic and transcriptional regulations as compared with Arabidopsis, nodule senescence and nitric oxide signalling. New Phytol 181:563–575CrossRefGoogle Scholar
  22. Delledonne M, Xia Y, Dixon RA, Lamb C (1998) Nitric oxide functions as a signal in plant disease resistance. Nature 394:585–588CrossRefGoogle Scholar
  23. Delledonne M, Zeier J, Marocco A, Lamb C (2001) Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response. Proc Natl Acad Sci USA 98:13454–13459CrossRefGoogle Scholar
  24. Durner J, Wendehenne D, Klessig DF (1998) Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose. Proc Natl Acad Sci USA 95:10328–10333CrossRefGoogle Scholar
  25. Fancy NN, Bahlmann AK, Loake GJ (2016) Nitric oxide function in plant abiotic stress. Plant Cell Environ 40:462–472CrossRefGoogle Scholar
  26. Feechan A, Kwon E, Yun BW, Wang Y, Pallas JA, Loake GJ (2005) A central role for S-nitrosothiols in plant disease resistance. Proc Natl Acad Sci USA 102:8054–8059CrossRefGoogle Scholar
  27. Ferrarini A, De Stefano M, Baudouin E, Pucciariello C, Polverari A, Puppo A, Delledonne M (2008) Expression of Medicago truncatula genes responsive to nitric oxide in pathogenic and symbiotic conditions. Mol Plant-Microbe Interact 21:781–790CrossRefGoogle Scholar
  28. Flatley J, Barrett J, Pullan ST, Hughes MN, Green J, Poole RK (2005) Transcriptional responses of Escherichia coli to S-nitrosoglutathione under defined chemostat conditions reveal major changes in methionine biosynthesis. J Biol Chem 280:10065–10072CrossRefGoogle Scholar
  29. Foyer CH, Noctor G (2016) Stress-triggered redox signalling: what’s in pROSpect? Plant Cell Environ 39:951–964CrossRefGoogle Scholar
  30. Gambino G, Boccacci P, Margaria P, Palmano S, Gribaudo I (2013) Hydrogen peroxide accumulation and transcriptional changes in grapevines recovered from flavescence doree disease. Phytopathology 103:776–784CrossRefGoogle Scholar
  31. Gross F, Durner J, Gaupels F (2013) Nitric oxide, antioxidants and prooxidants in plant defence responses. Front Plant Sci 4:419CrossRefGoogle Scholar
  32. He H, He L, Gu M (2014) The diversity of nitric oxide function in plant responses to metal stress. Biometals 27:219–228CrossRefGoogle Scholar
  33. Herrera-Vásquez A, Salinas P, Holuigue L (2015) Salicylic acid and reactive oxygen species interplay in the transcriptional control of defense genes expression. Front Plant Sci 6:171CrossRefGoogle Scholar
  34. Hess DT, Matsumoto A, Kim SO, Marshall HE, Stamler JS (2005) Protein S-nitrosylation: purview and parameters. Nat Rev Mol Cell Biol 6:150–166CrossRefGoogle Scholar
  35. Hu J, Huang X, Chen L, Sun X, Lu C, Zhang L, Wang Y, Zuo J (2015) Site-specific nitrosoproteomic identification of endogenously S-nitrosylated proteins in Arabidopsis. Plant Physiol 167:1731–1746CrossRefGoogle Scholar
  36. Huang X, von Rad U, Durner J (2002) Nitric oxide induces transcriptional activation of the nitric oxide-tolerant alternative oxidase in Arabidopsis suspension cells. Planta 215:914–923CrossRefGoogle Scholar
  37. Huang J, Wei H, Li L, Yu S (2018) Transcriptome analysis of nitric oxide-responsive genes in upland cotton (Gossypium hirsutum). PLoS One 13:e0192367CrossRefGoogle Scholar
  38. Hussain A, Mun BG, Imran QM, Lee SU, Adamu TA, Shahid M, Kim KM, Yun BW (2016) Nitric oxide mediated transcriptome profiling reveals activation of multiple regulatory pathways in Arabidopsis thaliana. Front Plant Sci 7:975PubMedPubMedCentralGoogle Scholar
  39. Imran QM, Hussain A, Lee SU, Mun BG, Falak N, Loake GJ, Yun BW (2018) Transcriptome profile of NO-induced Arabidopsis transcription factor genes suggests their putative regulatory role in multiple biological processes. Sci Rep 8:771CrossRefGoogle Scholar
  40. Kansanen E, Jyrkkanen H-K, Volger OL, Leinonen H, Kivela AM, Hakkinen S-K, Woodcock SR, Schopfer FJ, Horrevoets AJ, Yla-Herttala S (2009) Nrf2-dependent and-independent responses to nitro-fatty acids in human endothelial cells: identification of heat shock response as the major pathway activated by nitro-oleic acid. J Biol Chem 284:33233–33241CrossRefGoogle Scholar
  41. Kovacs I, Holzmeister C, Wirtz M, Geerlof A, Fröhlich T, Römling G, Kuruthukulangarakoola GT, Linster E, Hell R, Arnold GJ, Durner J, Lindermayr C (2016) ROS-Mediated inhibition of S-nitrosoglutathione reductase contributes to the activation of anti-oxidative mechanisms. Front Plant Sci 7:1669CrossRefGoogle Scholar
  42. Kumar RS, Shen CH, Wu PY, Kumar SS, Hua MS, Yeh KW (2016) Nitric oxide participates in plant flowering repression by ascorbate. Sci Rep 6:35246CrossRefGoogle Scholar
  43. Li SW, Leng Y, Shi RF (2017) Transcriptomic profiling provides molecular insights into hydrogen peroxide-induced adventitious rooting in mung bean seedlings. BMC Genomics 18:188CrossRefGoogle Scholar
  44. Lindermayr C (2018) Crosstalk between reactive oxygen species and nitric oxide in plants: key role of S-nitrosoglutathione reductase. Free Radic Biol Med 122:110–115CrossRefGoogle Scholar
  45. Lindermayr C, Durner J (2015) Interplay of reactive oxygen species and nitric oxide: nitric oxide coordinates reactive oxygen species homeostasis. Plant Physiol 167:1209–1210CrossRefGoogle Scholar
  46. Liu L, Hausladen A, Zeng M, Que L, Heitman J, Stamler JS (2001) A metabolic enzyme for S-nitrosothiol conserved from bacteria to humans. Nature 410:490–494CrossRefGoogle Scholar
  47. Marinho HS, Real C, Cyrne L, Soares H, Antunes F (2014) Hydrogen peroxide sensing, signaling and regulation of transcription factors. Redox Biol 2:535–562CrossRefGoogle Scholar
  48. Mata-Pérez C, Sánchez-Calvo B, Begara-Morales JC, Carreras A, Padilla MN, Melguizo M, Valderrama R, Corpas FJ, Barroso JB (2016a) Nitro-linolenic acid is a nitric oxide donor. Nitric Oxide 57:57–63CrossRefGoogle Scholar
  49. Mata-Pérez C, Sánchez-Calvo B, de las Padilla-Serrano MN, Begara-Morales JC, Luque F, Melguizo M, Jiménez-Ruiz J, Fierro-Risco J, Peñas-Sanjuan A, Valderrama R (2016b) Nitro-fatty acides in plant signaling: nitro-linolenic acid induces the molecular chaperone network in Arabidopsis. Plant Physiol 170:686–701CrossRefGoogle Scholar
  50. Miller GAD, Mittler RON (2006) Could heat shock transcription factors function as hydrogen peroxide sensors in plants? Ann Bot 98:279–288CrossRefGoogle Scholar
  51. Monzón GC, Pinedo M, Di Rienzo J, Novo-Uzal E, Pomar F, Lamattina L, de la Canal L (2014) Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses. Nitric Oxide 39:20–28CrossRefGoogle Scholar
  52. Mur LAJ, Mandon J, Persijn S, Cristescu SM, Moshkov IE, Novikova GV, Hall MA, Harren FJM, Hebelstrup KH, Gupta KJ (2013) Nitric oxide in plants: an assessment of the current state of knowledge. AoB Plant 5:pls052CrossRefGoogle Scholar
  53. Niu L, Liao W (2016) Hydrogen peroxide signaling in plant development and abiotic responses: crosstalk with nitric oxide and calcium. Front Plant Sci 7:230PubMedPubMedCentralGoogle Scholar
  54. Orozco-Cárdenas ML, Narváez-Vásquez J, Ryan CA (2001) Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate. Plant Cell 13:179–191CrossRefGoogle Scholar
  55. Ortega-Galisteo AP, Rodriguez-Serrano M, Pazmiño DM, Gupta DK, Sandalio LM, Romero-Puertas MC (2012) S-Nitrosylated proteins in pea (Pisum sativum L.) leaf peroxisomes: changes under abiotic stress. J Exp Bot 63:2089–2103CrossRefGoogle Scholar
  56. Palmieri MC, Sell S, Huang X, Scherf M, Werner T, Durner JR, Lindermayr C (2008) Nitric oxide-responsive genes and promoters in Arabidopsis thaliana: a bioinformatics approach. J Exp Bot 59:177–186CrossRefGoogle Scholar
  57. Parani M, Rudrabhatla S, Myers R, Weirich H, Smith B, Leaman DW, Goldman SL (2004) Microarray analysis of nitric oxide responsive transcripts in Arabidopsis. Plant Biotechnol J 2:359–366CrossRefGoogle Scholar
  58. Polverari A, Molesini B, Pezzotti M, Buonaurio R, Marte M, Delledonne M (2003) Nitric oxide-mediated transcriptional changes in Arabidopsis thaliana. Mol Plant-Microbe Interact 16:1094–1105CrossRefGoogle Scholar
  59. Procházková D, Sumaira J, Wilhelmová NA, Pavlíková D, Száková J (2014) Reactive nitrogen species and the role of NO in abiotic stress. In: Ahmad P (ed) Emerging technologies and managment of crops stress tolerance. Elsevier, LondonGoogle Scholar
  60. Puppo A, Pauly N, Boscari A, Mandon K, Brouquisse R (2013) Hydrogen peroxide and nitric oxide: key regulators of the legume-rhizobium and mycorrhizal symbioses. Antioxid Redox Signal 18:2202–2219CrossRefGoogle Scholar
  61. Quirino BF, Normanly J, Amasino RM (1999) Diverse range of gene activity during Arabidopsis thaliana leaf senescence includes pathogen-independent induction of defense-related genes. Plant Mol Biol 40:267–278CrossRefGoogle Scholar
  62. Radi R (2004) Nitric oxide, oxidants, and protein tyrosine nitration. Proc Natl Acad Sci USA 101:4003–4008CrossRefGoogle Scholar
  63. Romero-Puertas MC, Laxa M, Matté A, Zaninotto F, Finkemeier I, Jones AME, Perazzolli M, Vandelle E, Dietz KJ, Delledonne M (2007) S-nitrosylation of peroxiredoxin II E promotes peroxynitrite-mediated tyrosine nitration. Plant Cell 19:4120–4130CrossRefGoogle Scholar
  64. Sewelam N, Jaspert N, Van Der Kelen K, Tognetti VB, Schmitz J, Frerigmann H, Stahl E, Zeier J, Van Breusegem F, Maurino VG (2016) Spatial H2O2 signaling specificity: H2O2 from chloroplasts and peroxisomes modulates the plant transcriptome differentially. Mol Plant 7:1191–1210CrossRefGoogle Scholar
  65. Singh PK, Indoliya Y, Chauhan AS, Singh SP, Singh AP, Dwivedi S, Tripathi RD, Chakrabarty D (2017) Nitric oxide mediated transcriptional modulation enhances plant adaptive responses to arsenic stress. Sci Rep 7:3592CrossRefGoogle Scholar
  66. Su T, Wang P, Li H, Zhao Y, Lu Y, Dai P, Ren T, Wang X, Li X, Shao Q (2018) The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome derived signaling in plant development. J Integr Plant Biol 60:591–607CrossRefGoogle Scholar
  67. Tanou G, Job C, Rajjou L, Arc E, Belghazi M, Diamantidis G, Molassiotis A, Job D (2009) Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity. Plant J 60:795–804CrossRefGoogle Scholar
  68. Vandenabeele S, Van Der Kelen K, Dat J, Gadjev I, Boonefaes T, Morsa S, Rottiers P, Slooten L, Van Montagu M, Zabeau M (2003) A comprehensive analysis of hydrogen peroxide-induced gene expression in tobacco. Proc Natl Acad Sci USA 100:16113–16118CrossRefGoogle Scholar
  69. Vandenabeele S, Vanderauwera S, Vuylsteke M, Rombauts S, Langebartels C, Seidlitz HK, Zabeau M, Van Montagu M, Inzé D, Van Breusegem F (2004) Catalase deficiency drastically affects gene expression induced by high light in Arabidopsis thaliana. Plant J 39:45–58CrossRefGoogle Scholar
  70. Vanderauwera S, Zimmermann P, Rombauts S, Vandenabeele S, Langebartels C, Gruissem W, Inzé D, Van Breusegem F (2005) Genome-wide analysis of hydrogen peroxide-regulated gene expression in Arabidopsis reveals a high light-induced transcriptional cluster involved in anthocyanin biosynthesis. Plant Physiol 139:806–821CrossRefGoogle Scholar
  71. Wang P, Du Y, Hou YJ, Zhao Y, Hsu CC, Yuan F, Zhu X, Tao WA, Song CP, Zhu JK (2015) Nitric oxide negatively regulates abscisic acid signaling in guard cells by S-nitrosylation of OST1. Proc Natl Acad Sci USA 112:613–618CrossRefGoogle Scholar
  72. Wilhelm BT, Landry JR (2009) RNA-Seq-quantitative measurement of expression through massively parallel RNA-sequencing. Methods 48:249–257CrossRefGoogle Scholar
  73. Xiao J, Jin X, Jia X, Wang H, Cao A, Zhao W, Pei H, Xue Z, He L, Chen Q (2013) Transcriptome-based discovery of pathways and genes related to resistance against Fusarium head blight in wheat landrace Wangshuibai. BMC Genomics 14:197CrossRefGoogle Scholar
  74. Yang H, Mu J, Chen L, Feng J, Hu J, Li L, Zhou JM, Zuo J (2015) S-nitrosylation positively regulates ascorbate peroxidase activity during plant stress responses. Plant Physiol 167:1604–1615CrossRefGoogle Scholar
  75. Yu M, Lamattina L, Spoel SH, Loake GJ (2014) Nitric oxide function in plant biology: a redox cue in deconvolution. New Phytol 202:1142–1156CrossRefGoogle Scholar
  76. Yun BW, Feechan A, Yin M, Saidi NBB, Le Bihan T, Yu M, Moore JW, Kang JG, Kwon E, Spoel SH (2011) S-nitrosylation of NADPH oxidase regulates cell death in plant immunity. Nature 478:264–268CrossRefGoogle Scholar
  77. Zeidler D, Zähringer U, Gerber I, Dubery I, Hartung T, Bors W, Hutzler P, Durner J (2004) Innate immunity in Arabidopsis thaliana: lipopolysaccharides activate nitric oxide synthase (NOS) and induce defense genes. Proc Natl Acad Sci USA 101:15811–15816CrossRefGoogle Scholar
  78. Zeng F, Sun F, Li L, Liu K, Zhan Y (2014) Genome-scale transcriptome analysis in response to nitric oxide in birch cells: implications of the triterpene biosynthetic pathway. PLoS One 9:e116157CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Juan C. Begara-Morales
    • 1
  • Mounira Chaki
    • 1
  • Raquel Valderrama
    • 1
  • Capilla Mata-Pérez
    • 1
  • María Padilla
    • 1
  • Juan B. Barroso
    • 1
    Email author
  1. 1.Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Experimental Biology, Center for Advanced Studies in Olive Grove and Olive Oils, Faculty of Experimental SciencesUniversity of JaénJaénSpain

Personalised recommendations