Two-Stage Process to Enhance Bio-hydrogen Production

  • E. Judith MartínezEmail author
  • Daniel Blanco
  • Xiomar Gómez
Part of the Biofuel and Biorefinery Technologies book series (BBT, volume 9)


Bio-hydrogen is generated by renewable feedstocks from biological, chemical, thermochemical and photolytic methods. Biological methods such as dark fermentation have been suggested as a feasible alternative to produce this gas and obtain a sustainable energy source. Bio-hydrogen is not a primary energy source, but it is compatible with electrochemical and combustion processes for energy conversion; this gas can be stored, transported and utilised to fulfil energy needs, and it also contributes to minimise carbon-based emissions reducing environmental pollution and climate change. In the present manuscript, a review is performed about the state of the art of the dark fermentation process and its integration with other processes in an attempt to increase the efficiency of substrate conversion. The two-stage configurations studied involve the bioprocesses for hydrogen production and waste treatment by coupling the dark fermentation process with an alternative biological route such as anaerobic digestion, microbial electrochemical systems or photo-fermentation to promote an efficient stabilisation and use of the organic matter.


Bio-hydrogen Dark fermentation Anaerobic digestion Microbial electrochemical systems 



This research was possible thanks to the financial support of the Ministerio de Economía y Competitividad and Fondo Europeo de Desarrollo Regional through the project CTQ2015-68925-R.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • E. Judith Martínez
    • 1
    Email author
  • Daniel Blanco
    • 1
  • Xiomar Gómez
    • 1
  1. 1.Chemical and Environmental Bioprocess Engineering GroupNatural Resources Institute (IRENA), University of LeonLeonSpain

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