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An Amperometric Glucose Microbiosensor for Real-Time Measurements in Plants

  • Ye Hu
  • Cheng Wang
  • Aixue LiEmail author
Conference paper
Part of the IFIP Advances in Information and Communication Technology book series (IFIPAICT, volume 545)

Abstract

Glucose is not only as a main metabolic material, but also as a signaling molecule during the process of plant growth. In this work, for the first time, we developed an electrochemical microbiosensor suitable for real-time detecting of glucose concentration in plants. The biosensor uses glucose oxidase (GOD) to catalyze the glucose, and uses cellulose acetate (CA) and polycarbonate (PC) to improve the electrochemical selectivity and reduce the dependence of response on ambient oxygen. The linear range of the proposed sensor is wide and the detection limit is lower, which could basically meet the needs of plant testing. The content of glucose in sunflower under salt stress was monitored in real-time successfully, confirming the applicability of the fabricated microbiosensor. This work has a potential to open an innovative way to study the dynamic changes of physiological state of the plants in real-time.

Keywords

Glucose Glucose oxidase Sunflower Salt stress In vivo 

Notes

Acknowledgements

The authors are thankful for the funds from the National Natural Science Foundation of China (Grant No. 61571443), Scientific and Technological Innovation Team of Beijing Academy of Agricultural and Forestry Sciences (JNKYT201604).

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Copyright information

© IFIP International Federation for Information Processing 2019

Authors and Affiliations

  1. 1.Beijing Research Center of Intelligent Equipment for AgricultureBeijing Academy of Agriculture and Forestry SciencesBeijingChina
  2. 2.Beijing Research Center for Information Technology in AgricultureBeijing Academy of Agriculture and Forestry SciencesBeijingChina

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