Choosing the Right Technique
Brightfield microscopy is the technique that most people think of when they think of microscopy. It is indeed the most commonly used and usually the first technique that you will learn. It is limited, however, to samples that are thin and flat. You need not be restricted, and there are a range of techniques that are easy to use and accessible to the beginner. I have included the most common techniques here—brightfield, darkfield, phase contrast, Z-stacking, and stereomicroscopy. If you can master these five techniques, you will be very well equipped to deal with most kinds of sample.
We will see what each technique looks like, what sample it is best suited for, the tech specs for the microscope, and the sample requirements.