Bioelectrocatalytic Assessment of the Activating Effect and Toxic Interaction Between Carbon Nanomaterials and Microbial Cells
Carbon and other types of nanomaterials have found broad application in various fields of human activities. However, their impact on living organisms, including microorganisms, still needs to be understood more profoundly. Some nanomaterials cause activating effects, others are characterized by toxicity. The action of nanomaterials on microorganisms is assessed by bringing them into mutual contact by chemical immobilization, sorption or other techniques and registering the caused effect. Changes of the bioelectrocatalytic characteristics—the main parameters of the electrodes (anodes or working cathodes) in such devices as biosensors or microbial fuel cells (MFC)—are widely used for assessment. These characteristics are studied by cyclic voltammetry, chronoamperometry and potentiometry as well as impedance spectroscopy. In this chapter, we briefly describe the effects of mainly carbon nanomaterials on microorganisms. Emphasis is made on presenting data obtained for Gluconobacter , which is used as the basis of biosensors and MFC and can be considered as model biomaterial.
KeywordsCarbon nanomaterials Modification of graphite electrode Activating and toxic effects Immobilized Gluconobacter cells Bioelectrocatalytic testing Microbial fuel cell Biosensors
The authors are grateful for the support by the Russian Science Foundation within the framework of the project “Design, Fabrication and Study of New Hybrid Integrated Sensors Based on Nanoelectronic, Acoustoelectronic and Electrochemical Technologies for Biological Applications” No. 18-49-08005.
- Bard A, Faulkner L (2001) Electrochemical methods. In: Fundamentals and Application, 2nd ed. Wiley, New York, pp 368–414Google Scholar
- European Parliament and the Council of the European Union (2010) Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. Official Journal of the European Union L 276, vol. 53, pp 33–79Google Scholar
- Fogel R, Limson JL (2016) Applications of nanomaterials in microbial fuel cells. In: Ozoemena K, Chen S (eds) Nanomaterials for fuel cell catalysis. Nanostructure science and technology. Springer, Cham, pp 551–575Google Scholar
- Li L, Liang B, Li F, Shi JG, Mascini M, Lang QL, Liu A (2013) Co-immobilization of glucose oxidase and xylose dehydrogenase displayed whole cell on multiwalled carbon nanotube nanocomposite films modified electrode for simultaneous voltammetric detection of D-glucose and D-xylose. Biosens Bioelectron 42:156–162CrossRefGoogle Scholar
- Lim JW, Ha D, Lee J, Lee SK, Kim T (2015) Review of micro/nanotechnologies for microbial biosensors. Front Bioeng Biotechnol 3:61Google Scholar
- Reshetilov AN, Plekhanova YV, Tarasov SE, Kitova AE, Uteshev VK, Vasilov RG, Kolesov VV (2016) Sposob poluchenia electricheskoi energii s pomoshiu mikrobnogo biotoplivnogo elementa implantirovannogo v organism zhivoi travyanoi lyagushki Rana Temporaria. RU Patent No. 2599421, 15 Sept 2016Google Scholar
- Reshetilov AN, Plekhanova JV, Tarasov SE, Bykov AG, Gutorov MA, Alferov SV, Tenchurin TK, Chvalun SN, Orekhov AS, Shepelev AD, Gotovtsev PM, Vasilov RG (2017a) Evaluation properties of bioelectrodes based on carbon superfine materials containing model microorganisms Gluconobacter. Nanotechnol Russ 12:107–115CrossRefGoogle Scholar
- Šefčovičová J, Tkac J (2015) Application of nanomaterials in microbial-cell biosensor constructions. Chem Papers 69:42–53Google Scholar
- Talebi S, Ramezani F, Ramezani M (2010) Biosynthesis of metal nanoparticles by micro-organisms. Nanocon Olomouc 10:12–18Google Scholar