Nox2 and Nox4 Participate in ROS-Induced Neuronal Apoptosis and Brain Injury During Ischemia-Reperfusion in Rats

  • Jinjin Wang
  • Yin Liu
  • Haitao Shen
  • Haiying Li
  • Zhong Wang
  • Gang Chen
Part of the Acta Neurochirurgica Supplement book series (NEUROCHIRURGICA, volume 127)


Background: Previously studies have shown that Nox2 and Nox4, as members of nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase, Nox), participate in brain damage caused by ischemia-reperfusion (I/R). The aim of this study is to investigate the effects of specific chemical inhibitors of Nox2 and Nox4 on cerebral I/R-induced brain injury in rats.

Methods: At 0.5 h before MCAO surgery, the rats were pretreated with vehicle, Nox2 inhibitor (gp91ds-tat), and Nox4 inhibitor (GKT137831), respectively. After reperfusion for 24 h, the infarct sizes of brain tissues in rats in various groups are determined. The penumbra (ischemic) tissues are collected to measure ROS levels, neuronal apoptosis, and degeneration, as well as the integrity of the blood-brain barrier (BBB) in brain tissues of rats.

Results: gp91ds-tat and GKT137831 pretreatment significantly reduced the infarct sizes in brain tissues of rats, effectively suppressed I/R-induced increase in ROS levels, neuronal apoptosis and degeneration, and obviously alleviated BBB damage.

Conclusion: Under cerebral I/R conditions, Nox2 inhibitor (gp91ds-tat) and Nox4 inhibitor (GKT137831) can effectively play a protective role in the brain tissues of rats.


Cerebral ischemia-reperfusion Nox2 Nox4 Middle cerebral artery occlusion Reactive oxygen species 




Conflict of Interest: The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Jinjin Wang
    • 1
    • 2
  • Yin Liu
    • 1
    • 3
  • Haitao Shen
    • 1
  • Haiying Li
    • 1
  • Zhong Wang
    • 1
  • Gang Chen
    • 1
  1. 1.Department of Neurosurgery and Brain and Nerve Research LaboratoryThe First Affiliated Hospital of Soochow UniversitySuzhouChina
  2. 2.Department of NeurosurgeryJiangsu Shengze HospitalSuzhouChina
  3. 3.Department of NeurosurgerySuzhou Municipal HospitalSuzhouChina

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