Recent Advances in Breeding for Modified Fatty Acid Profile in Soybean Oil

  • Akshay Talukdar
  • M. Shivakumar
  • Subhash Chandra


Soybean is an important source of food, feed, and edible oil in the world. It contains about 20% oil and 40-45% protein. Besides saturated fatty acids, the soybean oil contains both monounsaturated and polyunsaturated fatty acid (PUFA). Presence of PUFA, linolenic acid in particular makes soy oil vulnerable to oxidative degradation and rancidity. Hydrogenation enhances storage stability but adds trans-fatty acid and makes soy oil a poor choice for healthy hearts. Conventional and molecular approaches have been employed to genetically alter the fatty acid profile of soybean oil. Quantitative trait loci (QTL) and genes have been characterized in various genotypes and being deployed to manipulate the fatty acid profile as desired. In this article, recent advances and future considerations for fatty acid modification in soybean oil have been discussed.


Soybean Fatty acid profile Hydrogenation Mutation breeding QTL 


  1. Alt JL, Fehr WR, Welke GA, Sandu D (2005a) Phenotypic and molecular analysis of oleate content in the mutant soybean line M23. Crop Sci 45:1997–2000CrossRefGoogle Scholar
  2. Alt JL, Fehr WR, Welke GA, Shannon JG (2005b) Transgressive segregation for oleate content in three soybean populations. Crop Sci 45:2005–2007CrossRefGoogle Scholar
  3. Bachlava E, Dewey RE, Burton JW, Cardinal AJ (2009) Mapping and comparison of quantitative trait loci for oleic acid seed content in two segregating soybean populations. Crop Sci 49:433–444CrossRefGoogle Scholar
  4. Belo A, Zheng P, Luck S, Shen B, Meyer DJ, Li B, Tingey S, Rafalski A (2008) Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize. Mol Gen Genomics 279:1–10CrossRefGoogle Scholar
  5. Bilyeu KD, Palavalli L, Sleper DA, Beuselinck PR (2003) Three microsomal omega-3 fatty-acid desaturase genes contribute to soybean linolenic acid levels. Crop Sci 43:1833–1838CrossRefGoogle Scholar
  6. Bin L, Shengxü F, Fukuan Y, Ying C, Shengrui Z, Fenxia H, Shurong Y, Lianzheng W (2017) Junming sun high-resolution mapping of QTL for fatty acid composition in soybean using specific-locus amplified fragment sequencing. Theor Appl Genet 130:1467–1479CrossRefGoogle Scholar
  7. Bino RJ, Hall RD, Fiehn O, Kopka J, Saito K, Draper J, Nikolau BJ, Mendes P, Roessner-Tunali U, Beale MH et al (2004) Potential of metabolomics as a functional genomics tool. Trends Plant Sci 9:418–425CrossRefGoogle Scholar
  8. Brummer EC, Nickell AD, Wilcox JR, Shoemaker RC (1995) Mapping the Fan locus controlling linolenic acid in soybean oil. J Hered 86:245–247CrossRefGoogle Scholar
  9. Buhr T, Sato S, Ebrahim F, Xing A, Zhou Y, Mathiesen M, Schweiger B, Kinney AJ, Staswick P, Clemente T (2002) Ribozyme termination of RNA transcripts down-regulate seed fatty acid genes in transgenic soybean. Plant J 30:155–163CrossRefGoogle Scholar
  10. Cerutti H (2003) RNA interference: traveling in the cell and gaining functions? Trends Genet 19:39–46CrossRefGoogle Scholar
  11. Chappell AS, Bilyeu KD (2006) A GmFAD3A mutation in the low linolenic acid mutant C1640. Plant Breed 125:535–536CrossRefGoogle Scholar
  12. Chi DW, Masatsugu H, Toyoaki A, Akihiro S, Ryo A (2017) Fatty acid composition and distribution in wild soybean (Glycine soja) seeds collected in Japan. Asian J Plant Sci 16(2):52–64. CrossRefGoogle Scholar
  13. Crystal BH, Jason DG (2017) Genotyping-by-sequencing-based investigation of the genetic architecture responsible for a sevenfold increase in soybean seed stearic acid. Genes Genomes Genet 7:299–308Google Scholar
  14. Diers BW, Shoemaker RC (1992) Restriction fragment length polymorphism of soybean fatty acid content. J Am Oil Chem Soc 69:1242–1244CrossRefGoogle Scholar
  15. Edgar BC, Thomas TE, Howard GD, Anthony JK (2009) Modifying vegetable oils for food and non-food purposes. In: Vollmann J, Rajcan I (eds) Oil crops, handbook of plant breeding, 4th edn. Springer, New York, NY. CrossRefGoogle Scholar
  16. Erickson EA, Wilcox JR, Cavins JF (1988) Inheritance of altered palmitic acid percentage in two soybean mutants. Heredity 79(6):465–468CrossRefGoogle Scholar
  17. Fan S, Li B, Yu F, Han F, Yan S, Wang L, Sun J (2015) Analysis of additive and epistatic quantitative trait loci underlying fatty acid concentrations in soybean seeds across multiple environments. Euphytica 206:689–700CrossRefGoogle Scholar
  18. Fehr WR (2007) Breeding for modified fatty acid composition in soybean. Crop Sci 47(3):72–87. CrossRefGoogle Scholar
  19. Fehr WR, Welke GA, Hammond EG, Duvick DN, Cianzio SR (1992) Inheritance of reduced linolenic acid content in soybean genotypes A16 and A17. Crop Sci 32:903–906CrossRefGoogle Scholar
  20. Flores T, Karpova O, Su X, Zheng P, Bilyeu K, Sleper DA, Nguyen HT, Zhang ZJ (2008) Silencing of the GmFAD3 gene by siRNA leads to low a-linolenic acids (18:3) of fad3-mutant phenotype in soybean Glycine max (Merr.). Transgenic Res 17:839–850CrossRefGoogle Scholar
  21. Graef G, LaVallee BJ, Tenopir P, Tat ME, Schweiger BJ, Kinney AJ, Van Gerpen J, Clemente TE (2009) A high oleic acid and low palmitic acid soybean: agronomic performance and evaluation as a feedstock for biodiesel. Plant Biotechnol J 7:411–421CrossRefGoogle Scholar
  22. Hajduch M, Ganapathy A, Stein JW, Thelen JJ (2005) A systematic proteomic study of seed filling in soybean: establishment of highresolution two-dimensional reference maps, expression profiles, and an interactive proteome database. Plant Physiol 137:1397–1419CrossRefGoogle Scholar
  23. Hammond EG, Fehr WR (1983) Registration of A5 germplasm line of soybean. Crop Sci 23:192Google Scholar
  24. Heppard EP, Ki nney AJ, Stecca KL, Miao GH (1996) Developmental and growth temperature regulation of two different microsomal o-6 desaturase genes in soybeans. Plant Physiol 110:311–319CrossRefGoogle Scholar
  25. Horejsi TF, Fehr WR, Welke GA, Duvick DN, Hammond EG, Cianzio SR (1994) Genetic control of reduced palmitate content in soybean. Crop Sci 34:331–334CrossRefGoogle Scholar
  26. Hu X, Sullivan-Gilbert M, Gupta M, Thompson SA (2006) Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canola (Brassica napus L.). Theor Appl Genet 113:497–507CrossRefGoogle Scholar
  27. Hyten DL, Pantalone VR, Saxton AM, Schmidt ME, Sams CE (2004) Molecular mapping and identification of soybean fatty acid modifier quantitative trait loci. J Am Oil Chem Soc 81:1115–1118CrossRefGoogle Scholar
  28. Istvan R, Guangyun H, Aron DW (2008) Advances in breeding of seed-quality traits in soybean. J Crop Improv 14(1-2):145–174Google Scholar
  29. Iyer VV, Sriram G, Fulton DB, Zhou R, Westgate ME, Shanks JV (2008) Metabolic flux maps comparing the effect of temperature on protein and oil biosynthesis in developing soybean cotyledons. Plant Cell Environ 31:506–517CrossRefGoogle Scholar
  30. Jackson SA, Rokhsar D, Stacey G, Shoemaker R, Schmutz J, Grimwood J (2006) Toward a reference sequence of the soybean genome: a multiagency effort. Plant Genome 46:S-55–S-61Google Scholar
  31. Jeong DL, Kristin DB, GS J (2007) Genetics and breeding for modified fatty acid profile in soybean seed oil. J Crop Sci Biotechnol 10(4):201–210Google Scholar
  32. Kim HJ, Ha B-K, Ha K-S, Chae J-H, Park J-H, Kim M-S, Asekova S, Grover Shannon J, Son C-K, Lee J-D (2015) Comparison of a high oleic acid soybean line to cultivated cultivars for seed yield, protein and oil concentrations. Euphytica 201:285–292. CrossRefGoogle Scholar
  33. Kinney AJ (1996) Development of genetically engineered soybean oils for food applications. J Food Lipids 3 273–292CrossRefGoogle Scholar
  34. Kinney AJ, Knowlton S (1997) Designer oils: the high oleic soybean. In: Harander S, Roller S (eds) Genetic engineering for food industry: a strategy for food quality improvement. Blackie Academic, London, pp 193–213Google Scholar
  35. Korver O, Katan MB (2006) The elimination of trans fats from spreads: how science helped to turn an industry around. Nutr Rev 64:275–279PubMedGoogle Scholar
  36. Li YH, Reif JC, Ma YS, Hong HL, Liu ZX, Chang RZ, Qiu LJ (2015) Targeted association mapping demonstrating the complex molecular genetics of fatty acid formation in soybean. BMC Genomics 16:841CrossRefGoogle Scholar
  37. Lichtenstein AH, Matthan NR, Jalbert SM, Resteghini NA, Schaefer EJ, Ausman LM (2006) Novel soybean oils with different fatty acid profiles alter cardiovascular disease risk factors in moderately hyperlipidemic subjects. Am J Clin Nutr 84:497–504CrossRefGoogle Scholar
  38. Masum A, Shiming L, Melanie B, Stella KK, Khalid M, Nacer B, David AL, Abdelmajid K (2014) Identification of quantitative trait loci (QTL) underlying protein, oil, and five major fatty acids’ contents in soybean. Am J Plant Sci 5:158–167CrossRefGoogle Scholar
  39. Mazur B, Krebbers E, Tingey S (1999) Gene discovery and product development for grain quality traits. Science 285:372–375CrossRefGoogle Scholar
  40. Mikkilineni V, Rocheford TR (2003) Sequence variation and genomic organization of fatty acid desaturase-2 (fad2) and fatty acid desaturase-6 (fad6) cDNAs in maize. Theor Appl Genet 106:1326–1332CrossRefGoogle Scholar
  41. Monteros MJ, Burton JW, Boerma HR (2008) Molecular mapping and confirmation of QTLs associated with oleic acid content in N00-3350 soybean. Crop Sci 48:2223–2234CrossRefGoogle Scholar
  42. Mozaffarian D, Willett WC (2007) Trans fatty acids and cardiovascular risk: a unique cardiometabolic imprint? Curr Atheroscler Rep 9:486–493CrossRefGoogle Scholar
  43. Panthee DR, Pantalone VR, Saxton AM (2006) Modifier QTL for fatty acid composition in soybean oil. Euphytica 152:67–73CrossRefGoogle Scholar
  44. Patel M, Jung S, Moore K, Powell G, Ainsworth C, Abbott A (2004) High-oleate peanut mutants result from a MITE insertion into the FAD2 gene. Theor Appl Genet 108:1492–1502CrossRefGoogle Scholar
  45. Perez-Vich B, Ferna ndez-Martınez JM, Grondona M, Knapp SJ, Berry ST (2002) Stearoyl- ACP and oleoyl-PC desaturase genes cosegregate with quantitative trait loci underlying high stearic and high oleic acid mutant phenotypes in sunflower. Theor Appl Genet 104:338–349CrossRefGoogle Scholar
  46. Primomo VS, Falk DE, Ablett GR, Tanner JW, Rajcan I (2002) Genotype X environment interactions, stability, and agronomic performance of soybean with altered fatty acid profiles. Crop Sci 42:37–44CrossRefGoogle Scholar
  47. Rahman SM, Kinoshita T, Anai T, Takagi Y (2001) Combining ability in loci for high oleic and low linolenic acids in soybean. Crop Sci 41:26–29CrossRefGoogle Scholar
  48. Rebetzke GJ, Burton JW, Carter TE Jr, Wilson RF (1998) Genetic variation for modifiers controlling reduced saturated fatty acid content in soybean. Crop Sci 38:303–308CrossRefGoogle Scholar
  49. Reinprecht Y, Poysa VW, Yu K, Rajcan I, Ablett GR, Pauls KP (2006) Seed and agronomic QTL in low linolenic acid, lipoxygenase-free soybean (Glycine max (L.) Merrill) germplasm. Genome 49:1510–1527CrossRefGoogle Scholar
  50. Reinprecht Y, Luk-Labey SY, Larsen J, Poysa VW, Yu K, Rajcan I, Ablett GR, Pauls KP (2009) Molecular basis of the low linolenic acid trait on soybean EMS mutant line RG10. Plant Breed 128:253–258CrossRefGoogle Scholar
  51. Shoemaker RC, Schlueter JA, Cregan P, Vodkinn L (2003) The status of soybean genomics and its role in the development of soybean biotechnologies. Ag Bio Forum 6(1&2):4–7Google Scholar
  52. Spencer MM, Pantalone VR, Meyer EJ, Landau-Ellis D, Hyten DL Jr (2003) Mapping the Fas locus controlling stearic acid contents in soybean. Theor Appl Genet 106:615–619CrossRefGoogle Scholar
  53. Stoltzfus DL, Fehr WR, Welke GA, Hammond EG, Cianzio SR (2000a) A fap5 allele for elevated palmiatate in soybean. Crop Sci 40:647–650CrossRefGoogle Scholar
  54. Stoltzfus DL, Fehr WR, Welke GA, Hammond EG, Cianzio SR (2000b) A fap7 allele for elevated palmiatate in soybean. Crop Sci 40:1538–1542CrossRefGoogle Scholar
  55. Takagi Y, Rahman SM (1996) Inheritance of high oleic acid content in the seed oil of soybean mutant M23. Theor Appl Genet 92:179–182CrossRefGoogle Scholar
  56. Tang GQ, Novitzky WP, Griffin HC, Huber SC, Dewey RE (2005) Oleate desaturase enzymes of soybean: evidence of regulation through differential stability and phosphorylation. Plant J 44:433–446CrossRefGoogle Scholar
  57. Tom EC, Edgar BC (2009) Soybean oil: genetic approaches for modification of functionality and total content. Plant Physiol 151:1030–1040CrossRefGoogle Scholar
  58. Wang X, Jiang GL, Green M, Scott RA, Hyten DL, Cregan PB (2012) Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean. Mol Breed 30:1163–1179CrossRefGoogle Scholar
  59. White HBJ, Quackenbush FW, Probst AH (1961) Occurrence and inheritance of linolenic and linoleic acids in soybean seeds. J Am Oil Chem Soc 38:113–117CrossRefGoogle Scholar
  60. Wilcox JR, Cavins JF (1985) Inheritance of low linolenic acid content of the seed oil of a mutant Glycine max. Theor Appl Genet 71:74–78CrossRefGoogle Scholar
  61. William H, Andrew C, Benjamin MC, Zachary LD, Anita O, Erin R, Adam R, Thomas S, Alexandre J, Frederic C, Luc M, Daniel FV, Feng Z (2014) Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family. Plant Biotechnol J 12:934–940CrossRefGoogle Scholar
  62. Xie D, Han Y, Zeng Y, Chang W, Teng W, Li W (2012) SSR-and SNPrelated QTL underlying linolenic acid and other fatty acid contents in soybean seeds across multiple environments. Mol Breed 30:169–179CrossRefGoogle Scholar
  63. Zhou Z, Jiang Y, Wang Z, Gou Z et al (2015) Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean. Nat Biotechnol 33(4):408–414CrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Akshay Talukdar
    • 1
  • M. Shivakumar
    • 2
  • Subhash Chandra
    • 2
  1. 1.Division of GeneticsICAR-Indian Agricultural Research InstituteNew DelhiIndia
  2. 2.ICAR-Indian Institute of Soybean ResearchIndoreIndia

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