Pathology Specimen: Handling Techniques

  • Hardeep Singh MudharEmail author


This chapter covers the handling of surgical ophthalmic pathology specimens in theatre and in the pathology laboratory. The handling of the specimens in theatre covers fresh tissue handling for frozen sections, which fixatives are required for the various specimens, orientation of specimens, and how to communicate clearly with pathology colleagues by filling out the pathology request form adequately.

The handling of the specimens in the pathology laboratory covers how pathologists approach the grossing/macroscopic examination of select specimens including eyelid tumour resections, mapping biopsies, fresh tissue destined for frozen tissue, cytology specimens, enucleation and exenteration specimens.


Specimen orientation Mapping biopsies Specimen handling Frozen sections Cytology Fine-needle aspiration (FNA) Vitreous biopsy Fixation Pathology request form Local resection Enucleation Exenteration 


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.National Specialist Ophthalmic Pathology Service (NSOPS), Department of Histopathology, E-Floor, Sheffield Teaching Hospitals NHS Foundation Trust, Royal Hallamshire HospitalSheffieldUK

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