Characterization of Human Semen by GC-MS and VOC Sensor: An Unexplored Approach to the Study on Infertility
Infertility is one of principal health and social problems of this century. Male factors are involved in half of the cases and often the alteration concerns sperm motility. Seminogram is the gold standard technique for semen analysis, but it presents several limits. For this reason, we propose a new method for discriminating asthenozoospermic samples (low sperm motility) from normozoospermic ones (progressive motility > 32%) based on the never explored analysis of the volatile metabolites in the headspace of human semen sample by Gas Chromatograph (GC) equipped with two detectors: a Mass Spectrometer (MS) and a metal oxide based gas sensor sensitive (MOX) to Volatile Organic Compounds (VOCs). VOC sensor signal profiles (resistance vs. time) showed a higher sensitivity to specific organic classes such as aldehydes and ketones. The sensorgrams were preprocessed and analysed by PLS-DA. The results showed that sensorgrams analysis by suitable bioinformatics techniques has a good discrimination power and could support physiological parameters in human semen assessment. The analysis of the human semen Volatilome may be a proof-of-concept for the development of a novel micro-GC device with a sensor array detector, a potential candidate for infertility assessment in clinical practice.
KeywordsHuman semen VOC sensor GC/MOX sensor system
The authors would like to thank Dott. Luigi Montano, coordinator of “EcoFood-Fertility”, for scientific support.
- 1.Rowe, P.J., Comhaire, F.H.: WHO Manual for the Standardized Investigation and Diagnosis of the Infertile Male. Cambridge University Press (2000)Google Scholar
- 3.WHO: WHO Laboratory Manual for the Examination and Processing of Human Semen. 5th edn. Cambridge University Press (2010)Google Scholar
- 5.Amann, A., De Lacy Costello, B., Miekisch, W., Schubert, J., Buszewski, B., Pleil, J., Ratcliffe, N., Risby, T.: The human volatilome: volatile organic compounds (VOCs) in exhaled breath, skin emanations, urine, feces and saliva. J. Breath Res. 8(3), 034001 (2014). https://doi.org/10.1088/1752-7155/8/3/034001CrossRefGoogle Scholar
- 9.Costello, B.D., Amann, A., Al-Kateb, H., Flynn, C., Filipiak, W., Khalid, T., Osborne, D,. Ratcliffe, N.M: A review of the volatiles from the healthy human body. J. Breath Res. 8(1) (2014)Google Scholar