One-Step Functionalization of Silicon Nanoparticles with Phage Probes to Identify Pathogenic Bacteria
Optical biosensors are powerful alternatives to the conventional analytical techniques, due to their particular high specificity, sensitivity, small size, and cost effectiveness. Although promising developments of optical biosensors are reported, new bioprobes of cheap and easy synthesis are required, for detection of eukaryotic cells or dangerous infectious agents. In this regard, silicon nanoparticles (SiNPs) can be used as nanoplatform owing to their high specific surface area, optical properties and biocompatibility. They can also be functionalized with bio-probes and used in diagnostic applications. Different methods are described to obtain a stable bond between SiNPs and probes such as nucleotides, antibodies or peptides; however, the latter show many disadvantages about folding instability and sensitivity during the functionalization. Phage Display is a technique for the screening and selection of peptide ligands, that uses an engineered filamentous bacteriophage, mostly made up of 2700 copies of a major coat protein (pVIII) displaying a foreign peptide specific for a target. The bacteriophage or its coat proteins alone can be used as probes to functionalize nanomaterials such as SiNPs. In this work, we propose a new approach to obtain fluorescent bio-probes that can be used for the realization of an optical biosensor. By pulsed laser ablation in liquid (PLAL), SiNPs are functionalized in a “one step” process with phages or isolated pVIII-engineered proteins, selective for Pseudomonas aeruginosa. This process led to complexation of SiNPs with both bioprobes proposed. The PLAL did not alter the biological function of phage probes, maintaining their binding capacity to the bacterial target.
KeywordsBiosensor Phage display M13 pVIII engineered proteins Silicon nanoparticles Pulsed laser ablation in liquid
- 1.Solano-Umaña, V., Vega-Baudrit, J.R., González-Paz, R.: The new field of the nanomedicine. Int. J. Appl. Sci. Technol. 5(1) (2015)Google Scholar
- 2.Huan, C., Shu-Qing, S.: Silicon nanoparticles: preparation, properties, and applications. In: Invited Review—International Conference on Nanoscience & Technology, China 2013, Chin. Phys. B 23(8), 088102 (2014)Google Scholar
- 6.Barbas III, C.F., Burton, D.R., Scott, J.K., Silverman, G.J.: Phage Display, A Laboratory Manual. Cold Spring Harbor Laboratory Press, Woodbury, NY (2001)Google Scholar
- 9.Liu, P., Han, L., Wang, F., Petrenko, V.A., Liu, A.: Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus. Biosens. Bioelectron. 82, 195–203 (2016). https://doi.org/10.1016/j.bios.2016.03.075CrossRefGoogle Scholar
- 15.Shemetov, A.A., Nabiev, I., Sukhanova, A.: Molecular Interaction of Proteins and Peptides with Nanoparticles, vol. 6. American Chemical Society (2012)Google Scholar