A Silicon-Based Biosensor for Bacterial Pathogens Detection
The miniaturization of integrated nucleic acid testing devices represents a critical step toward the development of portable systems able to offer sample-in-answer-out diagnostic analysis. The conventional molecular testing workflow involves laboratory infrastructures and well-trained staff. Here we present a versatile, user-friendly miniaturized Lab-on-Chip device for the molecular diagnostics of infectious diseases. It is composed of a polycarbonate ring and a silicon chip; a customized reader integrating electronic and optical modules was developed for driving the thermal and optical processes. We report the results obtained using our device for the sample processing and detection of gram-negative opportunistic pathogenic bacteria.
KeywordsPoint-of-care diagnostics Nucleic acid testing Infectious diseases
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