Immunological and Molecular Biological Methods

  • Vladimir V. Klimov


The reader can find the brief description of modern immunological and molecular biological techniques, principles of their execution, clinical applications, and rules of results evaluation in the individual context.

Supplementary material

466144_1_En_5_MOESM1_ESM.mp3 (9 mb)
(MP3 9234 kb)



  1. Adan A, Alizada G, Kiraz Y, Baran Y, Nalbant A. Flow cytometry: basic principles and applications. Crit Rev Biotechnol. 2017;37(2):163–76. Scholar
  2. Anwar MA, Tayyab MA, Kashif M, Afzal N. Paper based vs conventional enzyme linked immuno-sorbent assay: a review of literature. Int Clin Pathol J. 2016;3(3):00079. Scholar
  3. Gan SD, Patel KR. Enzyme immunoassay and enzyme-linked immunosorbent assay. J Investig Dermatol. 2013;133:e12. Scholar
  4. Han Y, Gu Y, Zhang AC, Lo Y-H. Review: imaging technologies for flow cytometry. Lab Chip. 2016;16:4639–47. Scholar
  5. Kabiraj A, Gupta J, Khaitan T, Bhattacharya PT. Principle and techniques of immunohistochemistry – a review. Int J Biol Med Res. 2015;6(3):5204–10.Google Scholar
  6. Kraaij T, Tengströma FC, Kamerling SA, et al. A novel method for high-throughput detection and quantification of neutrophil extracellular traps reveals ROS-independent NET release with immune complexes. Autoimmun Rev. 2016;15:577–84. Scholar
  7. Wang C, Wu J, Zong C, Xu J, Ju H-X. Chemiluminescent immunoassay and its applications. Chin J Anal Chem. 2012;40(1):3–10. Scholar

Landmark Papers

  1. Karmi O, Zayed A, Baraghethi S, Qadi M, Ghanem R. Measurement of vitamin B12 concentration: a review on available methods. IIOAB J. 2011;2(2):23–32.Google Scholar
  2. Kim JS, Kim SH, Kwon B, Hong S. Comparison of immunogenicity test methods used in clinical studies of infliximab and its biosimilar (CT-P13). Expert Rev Clin Immunol. 2015;11(S1):33–41. Scholar

Suggested Reading

  1. Mo Y, Wan R, Zhang Q. Application of reverse transcription-PCR and real-time PCR in nanotoxicity research. Methods Mol Biol. 2012;926:99–112. Scholar
  2. Rebernick R, Fahmy L, Glover C, et al. DNA area and NETosis analysis (DANA): a high-throughput method to quantify neutrophil extracellular traps in fluorescent microscope images. Biol Proced Online. 2018;20(7):1–9. Scholar
  3. Rohit A, Maiti B, Shenoy S, Karunasagar I. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for rapid diagnosis of neonatal sepsis. Indian J Med Res. 2016;143:72–8. Scholar
  4. Smith SM, Wunder MB, Norris DA, Shellman YG. A simple protocol for using a LDH-based cytotoxicity assay to assess the effects of death and growth inhibition at the same time. PLoS One. 2011;6(11):e26908. Scholar
  5. Taylor SC, Posch A. The design of a quantitative western blot experiment. Biomed Res Int. 2014;361590:1–8. Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Vladimir V. Klimov
    • 1
  1. 1.Clinical Immunology and Allergy DepartmentSiberian State Medical UniversityTomskRussia

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