Automated Segmentation and Computation of the Leukocytes Based on Morphological Operator

  • L. Vijay Mani Shankar
  • V. Mahesh
  • B. GeethanjaliEmail author
  • R. Subashini
Conference paper
Part of the Lecture Notes on Data Engineering and Communications Technologies book series (LNDECT, volume 26)


Cell image segmentation becomes important and yet difficult task in quantitative cytopathology. The main objective is to develop an algorithm to segment and calculate the amount of neutrophils using morphological operators. The current work focuses on extraction of neutrophils from the peripheral blood smear was taken and it’s stained using Leishman stain to obtain differential leukocyte count. The particle analysis is done by extorting the edges to isolate the appropriate elements from the surrounding image after suitable thresholding technique. The preliminary results in this study reveals the potentials of using particle analysis method in cell image segmentation for automation and further used for classifying.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • L. Vijay Mani Shankar
    • 1
  • V. Mahesh
    • 1
  • B. Geethanjali
    • 1
    Email author
  • R. Subashini
    • 1
  1. 1.Department of Biomedical Engineering, SSN College of EngineeringChennaiIndia

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