Advances in Molecular Diagnostic Approaches for Biothreat Agents

  • Meghana Rastogi
  • Sunit K. Singh


The advancement in Molecular techniques has been implicated in the development of sophisticated, high-end diagnostic platform and point-of-care (POC) devices for the detection of biothreat agents. Different molecular and immunological approaches such as Immunochromatographic and lateral flow assays, Enzyme-linked Immunosorbent assays (ELISA), Biosensors, Isothermal amplification assays, Nucleic acid amplification tests (NAATs), Next Generation Sequencers (NGS), Microarrays and Microfluidics have been used for a long time as detection strategies of the biothreat agents. In addition, several point of care (POC) devices have been approved by FDA and commercialized in markets. The high-end molecular platforms like NGS and Microarray are time-consuming, costly, and produce huge amount of data. Therefore, the future prospects of molecular based technique should focus on developing quick, user-friendly, cost-effective and portable devices against biological attacks and surveillance programs.


Point of care diagnostics Molecular diagnostics Portable diagnostic devices Biodefense and diagnostics Biothreat agents and diagnostics 


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Meghana Rastogi
    • 1
  • Sunit K. Singh
    • 1
  1. 1.Molecular Biology UnitInstitute of Medical Sciences, Banaras Hindu University (BHU)VaranasiIndia

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