A Biotechnological Method for Breeding Grapes Using in Vitro Growth Stimulants
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The aim of the research was to found grape breeding method that allows obtaining virus-free plants during breeding. It used the apical meristem for grapes breeding which is usually free from viruses. The conducted observations showed that a part of meristems was necrotic during the first stage of cultivation (2 weeks). The remaining meristems have evolved into micropiles of 2–2.5 mm which were re-transplanted. One month after planting, the surviving apical meristems were transplanted into a nutrient medium containing the same components. The transplantation was carried out in biological test tubes performed during 45–55 days for formation of regenerants with a size of 6–10 cm and obtaining microclones. The obtained material quality was measured according to growth regulators implemented such as 6-benzylaminopurine (6-BAP) and gibberellic acid (GA). It was found the microshoots grown on a medium with GA and 6-BAP concentration of 0.5 mg/l developed the most effective and virus free grape plants. 40% of the survival rate of the apical meristems was reached which allowed their further cultivation and propagation aiming at the production of virus-free planting material.
KeywordsGrapes Plant reproduction Nutrient medium Growth regulators
The results were obtained in the framework of the RFBR no. 16-35-60051, Ministry of Education and Science of the Russian Federation No. 6.6222.2017/8.9, President of Russian Federation no. MК-3476.2017.5.
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