Fluorescence Made Easier: Fluorescence Techniques for the Novice Episode 3:. Avoiding Those Pesky Artifacts: Sources of Error in Spectra and Steady State Measurements
For those contemplating employing fluorescence methods in their experiments, there are some potential artifacts and sources of error that can invalidate results. With a little care, these artifacts can be avoided to produce the kind of results that will make the reviewers jump for joy (and accept your paper). We will cover some sources of error arising from issues arising from the sample itself, and basic problems with fluorometers. At the end we have a handy checklist for when your spectrum comes out looking really messed up.
KeywordsRaman scattering Rayleigh scattering Inner filter effects Instrument troubleshooting Artifacts Sample purity
RBT wishes to belatedly thank Peter M. Torgerson (among many who taught him fluorescence) for the concept of the Golden Rules.
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