Circadian Regulation of Pineapple CAM Photosynthesis
- 361 Downloads
The altered carbon assimilation pathway of crassulacean acid metabolism (CAM) evolved as an adaptation to arid environments, and it confers 80% greater water use efficiency than the C3 pathway. Pineapple is the most economically valuable crop possessing CAM, and its wealth of genetic and genomic resources make it an excellent model for dissecting the molecular basis of this important pathway. Decades of physiology studies in pineapple identified the underlying biochemistry of CAM, but this work yielded little insight into the regulation and circadian control of CAM. High-resolution transcriptome surveys across the pineapple leaf gradient and throughout a diurnal time course were recently collected. This work uncovered new CAM-associated genes and suggested CAM evolved through neofunctionalization of existing gene copies and not through gene duplication events. CAM pathway genes are enriched with cis-regulatory elements associated with regulation by circadian clock genes. This includes novel clock-associated cis-regulatory elements in several stomatal movement genes, which may be related to nocturnal stomatal conductance in CAM plants. Together, these resources in pineapple provide a foundation for targeted engineering of CAM into C3 crop species.
KeywordsPineapple Diurnal Time Course Stomatal Movement CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) ATP-binding Cassette C11 (ABCC5)
- Brandt B, Brodsky DE, Xue S, Negi J, Iba K, Kangasjarvi J, Ghassemian M, Stephan AB, Hu H, Schroeder JI (2012) Reconstitution of abscisic acid activation of SLAC1 anion channel by CPK6 and OST1 kinases and branched ABI1 PP2C phosphatase action. Proc Natl Acad Sci U S A 109:10593–10598CrossRefGoogle Scholar
- Grew N, Rawlins W (1682) The anatomy of plants with an idea of a philosophical history of plants, and several other lectures, read before the Royal Society. W. Rawlins, LondonGoogle Scholar
- Matrosova A, Bogireddi H, Mateo-Penas A, Hashimoto-Sugimoto M, Iba K, Schroeder JI, Israelsson-Nordstrom M (2015) The HT1 protein kinase is essential for red light-induced stomatal opening and genetically interacts with OST1 in red light and CO2 -induced stomatal movement responses. New Phytol 208:1126–1137CrossRefGoogle Scholar
- Ming R, VanBuren R, Wai CM, Tang H, Schatz MC, Bowers JE, Lyons E, Wang ML, Chen J, Biggers E, Zhang J, Huang L, Zhang L, Miao W, Ye Z, Miao C, Lin Z, Wang H, Zhou H, Yim WC, Priest HD, Zheng C, Woodhouse M, Edger PP, Guyot R, Guo HB, Guo H, Zheng G, Singh R, Sharma A, Min X, Zheng Y, Lee H, Gurtowski J, Sedlazeck FJ, Harkess A, McKain MR, Liao Z, Fang J, Liu J, Zhang X, Zhang Q, Hu W, Qin Y, Wang K, Chen LY, Shirley N, Lin YR, Liu LY, Hernandez AG, Wright CL, Bulone V, Tuskan GA, Heath K, Zee F, Moore PH, Sunkar R, Leebens-Mack JH, Mockler T, Bennetzen JL, Freeling M, Sankoff D, Paterson AH, Zhu X, Yang X, Smith JA, Cushman JC, Paull RE, Yu Q (2015) The pineapple genome and the evolution of CAM photosynthesis. Nat Genet 47:1435–1442CrossRefGoogle Scholar