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Control of Pressure-Driven Microdroplet Formation and Optimum Encapsulation in Microfluidic System

  • Mathias Girault
  • Akihiro Hattori
  • Hyonchol Kim
  • Kenji Matsuura
  • Masao Odaka
  • Hideyuki Terazono
  • Kenji YasudaEmail author
Conference paper

Abstract

Formation of stable micro-droplets in multiphase flow is an important step to perform numerous microfluidic applications such as sorting experiments. We herein investigate the conditions of formation of stable micro-droplets using a flow focusing microfluidic device. Two single phases and four different multiphase flow regimes were observed depending on the pressures of fluids. By tuning sample stream pressure against fixed lower oil stream pressure, stable droplet regime can create microenvironment with a diameter ranged from 30 µm to 140 µm. Results obtained show that the formation of strictly size controlled droplets can encapsulate single cell-sized bead into droplet. Moreover, the limit between unstable and stable droplet regimes was the most suitable to efficiently encapsulate cell-sized bead in droplet sorting application. This limit can be precisely monitored by using the change of the droplet speed found at the threshold between these two regimes.

Keywords

Microfluidics Encapsulation Flow control 

Notes

Acknowledgements

This work was supported by Kanagawa Prefector’s local basic science funding for the On-chip Cellomics Project at the Kanagawa Academy of Science and Technology. This research was also supported by JST, CREST. We gratefully thank Ms. H. Mikami and Ms. M. Hasegawa for their helps during the experiments.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Mathias Girault
    • 1
  • Akihiro Hattori
    • 1
  • Hyonchol Kim
    • 1
    • 2
  • Kenji Matsuura
    • 1
  • Masao Odaka
    • 1
    • 2
  • Hideyuki Terazono
    • 2
  • Kenji Yasuda
    • 3
    • 4
    • 5
    Email author
  1. 1.Kanagawa Academy of Science and TechnologyTakatsuJapan
  2. 2.Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental UniversityChiyodaJapan
  3. 3.Department of Physics, Faculty of Science and EngineeringWaseda UniversityShinjuku-kuJapan
  4. 4.Waseda Bioscience Research Institute in Singapore (WABIOS)HeliosSingapore
  5. 5.CREST, Japan Science and Technology AgencyKawaguchiJapan

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