Animal Protein–Free Expansion of Human Mesenchymal Stem/Progenitor Cells
Human mesenchymal stem/progenitor cells (MSPCs) are currently used in a large number of clinical trials aimed at regeneration or immune modulation. Despite a growing body of knowledge about MSPC biology and function, fundamental questions regarding the optimal manipulation procedure still remain unresolved. Due to biological, ethical, and safety reasons, the use of fetal bovine serum as a standard cell culture supplement has come under dispute inducing a search for comparable alternatives. A humanized culture system requires not only animal serum–free supplements for basal media but also animal protein–free additives like coating reagents or trypsin preparations. In comparison to other blood-derived substances, pooled human platelet lysate (pHPL) has been proven to be the most efficient stimulant for MSPC proliferation enabling the expansion of sufficiently high cell yields within a suitable timeframe. In this chapter, we introduce alternatives to avoid animal-derived factors in the clinical-grade MSPC production with a special emphasis on pHPL as a novel and promising cell culture supplement.
KeywordsAnimal Protein Bone Marrow Mononuclear Cell Cumulative Population Doubling Bone Marrow Aspirate Concentrate Human Platelet Lysate
This work was supported by the Austrian Research Foundation (FWF, grant N211-NAN; DS) and the Adult Stem Cell Research Foundation (KS). The authors thank Claudia Url, Daniela Thaler, Margret Frühwirth, and Birgit Feilhauer for excellent technical assistance and Monica Farrell for editorial assistance.
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