Methods for Dynamical Inference in Intracellular Networks

  • Eleftheria Tzamali
  • Panayiota Poirazi
  • Martin ReczkoEmail author


Equation-based algorithms make hypotheses regarding the biophysical dynamical laws that govern a biological system and in the form of a mathematical expression, aiming to interrelate the system components, in an effort to explain and verify the experimental observations. This approach is what we mainly regard as dynamical inference. Assumptions such as the deterministic or stochastic laws that govern the system dynamics, the degree of modeling spatial phenomena, the exact mathematical representations of these biophysical laws and constraints, comprise some of the main issues of the dynamical inference problem. Another class of algorithms considers the cell as a whole system that orchestrates its components under physio-chemical constraints towards the accomplishment of certain cellular functions. These approaches avoid the search of detailed equation forms as well as the demand of knowledge of the parameters involved in the kinetics, and produce a steady state dynamic picture of the complex, genome-scale metabolic network of chemical reactions at the flux level. The constraint-based methods are essential for the analysis of the metabolic capabilities of organisms as well as the elucidation of systemic properties that cannot be described by descriptions of individual components or sub-systems.

The current biological knowledge, the available data and the computer power, are the issues that actually determine the upper limit for the system size and its complexity that can be simulated, thus defining our level of understanding.


Differential equations Stochastic simulation Spatial organization Constraint-based methods Flux balance analysis 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Eleftheria Tzamali
    • 1
  • Panayiota Poirazi
    • 2
  • Martin Reczko
    • 3
    Email author
  1. 1.University of CreteHeraklionGreece
  2. 2.Foundation for Research and Technology-HellasHeraklionGreece
  3. 3.Institute of Computer Science (ICS), Foundation of Research and Technology-Hellas (FORTH), Vassilika VoutonHeraklionGreece

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