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The Human Plasma and Serum Proteome

  • Gilbert S. Omenn
  • Rajasree Menon
  • Marcin Adamski
  • Thomas Blackwell
  • Brian B. Haab
  • Weimin Gao
  • David J. States

Abstract

Human plasma and serum are the preferred specimens for noninvasive studies of normal and disease-associated proteins in the circulation and arising from cells throughout the body. The attributes of extreme complexity, very wide dynamic range, genetic and physiological variation, endogenous and ex vivo modifications, and incompleteness of sampling by mass spectrometry all represent major challenges to reproducible, high-resolution, high-throughput analyses of the plasma proteome. This chapter summarizes the major reports to date identifying proteins in normal individuals and identifies paths to increased use of proteomics methods with human specimens for biomarker discovery and application in various diseases.

Key Words

Plasma proteome complexity dynamic range abundance variation posttranslational modifications splice isoforms ex vivo modification high-throughput error estimation 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Gilbert S. Omenn
    • 1
  • Rajasree Menon
    • 2
  • Marcin Adamski
    • 2
  • Thomas Blackwell
    • 2
  • Brian B. Haab
    • 3
  • Weimin Gao
    • 4
  • David J. States
    • 5
  1. 1.Michigan Proteomics Alliance for Cancer Research, Center for Computational Medicine and Biology, Departments of Internal Medicine and Human GeneticsUniversity of Michigan Medical SchoolAnn Arbor
  2. 2.Michigan Proteomics Alliance for Cancer Research, Center for Computational Medicine and BiologyUniversity of Michigan Medical SchoolAnn Arbor
  3. 3.Michigan Proteomics Alliance for Cancer ResearchVan Andel Research InstituteGrand Rapids
  4. 4.Michigan Proteomics Alliance for Cancer ResearchUSA
  5. 5.Michigan Proteomics Alliance for Cancer Research, Center for Computational Medicine and Biology, Department of Human GeneticsUniversity of Michigan Medical SchoolAnn Arbor

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