Biosurfactant Production by Rhodococcus erythropolis Grown on Glycerol As Sole Carbon Source

  • Elisa M. P. Ciapina
  • Walber C. Melo
  • Lidia M. M. Santa Anna
  • Alexandre S. Santos
  • Denise M. G. Freire
  • Nei PereiraJr.
Part of the ABAB Symposium book series (ABAB)


The production of biosurfactant by Rhodococcus erythropolis during the growth on glycerol was investigated. The process was carried out at 28°C in a 1.5-L bioreactor using glycerol as carbon source. The bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. After 51 h of cultivation, 1.7 g/L of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mN/m, respectively, 67% of Emulsifying Index (E 24), and 94% of oil removal were obtained. The use of glycerol rather than what happens with hydrophobic carbon source allowed the release of the biosurfactant, originally associated to the cell wall.

Index Entries

Biosurfactant Rhodococcus oil removal 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Elisa M. P. Ciapina
    • 1
  • Walber C. Melo
    • 1
  • Lidia M. M. Santa Anna
    • 2
  • Alexandre S. Santos
    • 1
  • Denise M. G. Freire
    • 3
  • Nei PereiraJr.
    • 1
  1. 1.Departamento de BioquímicaUniversidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco ERio de Janeiro, RJBrasil
  2. 2.Cenpes, PetrobrásRio de Janeiro, RJBrasil
  3. 3.Instituto de QuímicaUniversidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco ARio de Janeiro, RJBrasil

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