Optimization of Acid Hydrolysis of Sugarcane Bagasse and Investigations on its Fermentability for the Production of Xylitol by Candida guilliermondii

  • Rafael Fogel
  • Rafaela Rodrigues Garcia
  • Rebeca da Suva Oliveira
  • Denise Neves Menchero Palacio
  • Luciana da Silva Madeira
  • Eni PereiraJr.
Part of the ABAB Symposium book series (ABAB)


The dilute-acid hydrolysis of sugarcane bagasse was optimized using a statistical experimental design resulting in hydrolysates containing 57.25 g/L of xylose, which were fermented with a high inoculum concentration (10 g/L of the yeast Candida guilliermondii IM/UFRJ 50088). The addition of urea reduced the time of conversion (tC) to 75 h (without nitrogen source addition t C > 127 h), and, consequently, improving the rates of xylitol bioproduction. Fermentator experiments, using the optimized conditions, resulted in enhanced conversion rates, reducing t C to 30 h. The stability of the yeast in the hydrolysate was also verified in a 480-h cultivation.

Index Entries

Sugarcane bagasse dilute-acid hydrolysis optimization sugarcane bagasse hydrolysate xylitol bioproduction 


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Rafael Fogel
  • Rafaela Rodrigues Garcia
  • Rebeca da Suva Oliveira
  • Denise Neves Menchero Palacio
  • Luciana da Silva Madeira
  • Eni PereiraJr.
    • 1
  1. 1.Departamento de Engenharia Bioquímica, Escola de Química/CTUniversidade Federal do Rio de Janeiro, RJBrazil

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