Plasticity of Astrocytes in Culture

Comparison of Astrocytes Derived from Chick Embryonic Brain and Aged Mouse Brain
  • Antonia Vernadakis
  • Susan Kentroti
Chapter
Part of the Contemporary Neuroscience book series (CNEURO)

Abstract

Neuronal plasticity has been primarily attributed to the stage in early development when neuroblasts can be triggered to express a variety of neuronal phenotypes (Patterson and Chun, 1977a,b; Doupe et al., 1985; Wolinsky et al., 1985; Kentroti and Vernadakis, 1990, 1992, 1995). Neuronal plasticity has also been encountered in the adult CNS as several recent studies on regeneration have shown (see also Chapter 9, in this volume).

Keywords

Glial Cell Radial Glia Radial Glial Cell Mature Astrocyte Glial Precursor 
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References

  1. Abbott NJ (ed.) (1991) Glia-Neuronal Interactions, vol. 633, Ann NY Acad Sci, New York.Google Scholar
  2. Abney ER; Williams BP; Raff MC (1983) Tracing the development of oligodendrocytes from precursor cells using monoclonal antibodies, fluorcence activated cell sorting, and cell cultures. Dev Biol 199: 166–171.Google Scholar
  3. Aizenman Y; Weischel ME; de Vellis J (1986) Changes in insulin and transferrin requirements of pure brain neuronal cultures during embryonic development. Proc Natl Acad Sci USA 81: 2263–2266.Google Scholar
  4. Aloisi F; Agresti C; D’Urso D; Levi G (1988) Differentiation of bipotental glial precursors into oligodendrocytes is promoted by interaction with type 1 astrocytes in cerebellar cultures. Proc Natl Acad Sci USA 85: 6167–6171.PubMedGoogle Scholar
  5. Alvarez-Buylla A; Nottenbohm F (1988) Migration of young neurons in adult avian brain. Nature 335: 353–354.PubMedGoogle Scholar
  6. Alvarez-Buylla A; Theelen M; Nottenbohm F (1988) Mapping of radial glia and a new cell type in adult canary brain. J Neurosci 8: 2707–2712.PubMedGoogle Scholar
  7. Alvarez-Buylla A; Theelen M; Nottenbohm F (1990) Proliferation “hot spots” in adult avian ventricular zone reveal radial cell division. Neuron 5: 101–109.PubMedGoogle Scholar
  8. Barakat-Walter L; Deloulme JC; Sensenbrenner M; Labourdette G (1991) Proliferation of chick embryo neuroblasts grown in the presence of horse serum requires exogenous transferrin. J Neurosci Res 28: 391–398.PubMedGoogle Scholar
  9. Bignami A; Dahl D (1973) Differentiation of astrocytes in cerebellar cortex and the pyramidal traits in the newborn rat: An Immunofluorescence study with antibodies to a protein specific to astrocytes. Brain Res 49: 393–405.PubMedGoogle Scholar
  10. Bignami A; Dahl D (1976) The astroglia response to stabbing: Immunofluorescence studies with antibodies to astrocyte—specific protein ( GFA) in mammalian and submammalian vertebrates. J Neuropathol Appl Neurobiol 2: 99–110.Google Scholar
  11. Bignami A; Perides G (1991) Brain extracellular matrix. Adv Struct Biol 1: 1–20.Google Scholar
  12. Blakemore WF; Franklin RJM (1991) Transplantation of glial cells into the CNS. Trends Neurosci 4: 323–327.Google Scholar
  13. Calvo JL; Carbonell AL; Boya JC (1991) Co-expression of glia fibrillary acidic protein and vimentin in reactive astrocytes following brain injury in rats. Brain Res 566: 333–336.PubMedGoogle Scholar
  14. Cameron RS; Rakic P (1991) Glial cell lineage in the cerebral cortex: A review and synthesis. Glia 4: 124–137.PubMedGoogle Scholar
  15. Carbonetto S (1994) The extracellular matrix of the nervous system. Trends Neurosci 7: 382–387.Google Scholar
  16. Cardone B; Roots BI (1990) Comparative immunohistochemical study of glial filament proteins ( Glial fibrillary acidic protein and vimentin) in goldfish, octopus, and snail. Glia 3: 180–192.PubMedGoogle Scholar
  17. Cepko CL (1989) Immortalization of neural cells via retrovirus-modiated oncogene transduction. Ann Rev Neurosci 12: 47–65.PubMedGoogle Scholar
  18. Chao CC; Hu S; Tsang M; Weatherbee J; Molitov TW; Anderson WR; Peterson K (1992) Effects of transforming growth factor-f3 on murine astrocyte glutamine synthetase activity: Implications in neuronal injury. J Clin Invest 90: 1786–1793.PubMedGoogle Scholar
  19. Clarke DW; Boyd FT Jr.; Kappy MS; Raizada MK (1984) Insulin binds to specific receptors and stimulates 2-deoxy-D-glucose uptake in cultured glial cells from rat brain. J Biol Chem 259: 11672–11675.PubMedGoogle Scholar
  20. Connors BW; Ransom BR (1987) Electrophysiological properties of ependymal cells (radial glia) in dorsal cortex of the turtle, Pscademy Scripta. J Physiol (Lond) 385: 287–306.Google Scholar
  21. Cotman CW; Scheff SW (1979) Compensatory synapse growth in aged animals after neuronal death. Mech Ageing Dev 9: 103–117.PubMedGoogle Scholar
  22. Dahl D (1981) The vimentin-GFA protein transition in rat neuroglia cytoskeleton occurs at the time of myelination. J Neurosci Res 6: 741–748.PubMedGoogle Scholar
  23. Dahl D; Bignami A; Weber K; Osborn M (1981a) Filament proteins in rat optic nerves undergoing Wallerian degeneration: Localization of vimentin, the fibroblastic 100A filament proteins in normal and reactive astrocytes. Exp Neurol 73: 496–506.PubMedGoogle Scholar
  24. Dahl D; Rueger DC; Bignami A; Weber K; Osborn M (1981b) Vimentin, the 57,000 daltons protein of fibroblast filaments, is the major cytoskeletal component of immature glia. Eur J Cell Biol 24: 191–196.PubMedGoogle Scholar
  25. Doupe AJ; Patterson PA; Landis SC (1985) Small intensely fluorescent cells in culture: Role of glucocorticoids and growth factors in their development and interconversions with other neural crest derivatives. J Neurosci 5: 2143–2160.PubMedGoogle Scholar
  26. Eisenbarth GS; Walsh FS; Nirenberg M (1979) Monoclonal antibody to a plasma membrane antigen of neurons. Proc Natl Acad Sci USA 76: 4913–4917.PubMedGoogle Scholar
  27. Espinosa de los Monteros A; Chiapelli F; Fisher RS; de Vellis J (1988) Transfer-rin: An early marker of oligodendrocytes in culture. Int J Dev Neurosci 6: 167–175.Google Scholar
  28. Espinosa de los Monteros A; Kumar S; Cole R; de Vellis J (1990) Transferrin gene expression and secretion by rat brain cells in vitro. J Nerurosci Res 25: 576–580.Google Scholar
  29. Evrard C; Borde I; Marin P; Galiana E; Premont J; Gros F; Rouget P (1990) Immortalization of bipotential and plastic glia-neuronal precursor cells. Proc Natl Acad Sci USA 87: 3062–3066.PubMedGoogle Scholar
  30. Fedoroff S (1986) Prenatal ontogenesis of astrocytes, in Astrocytes, Development, Morphology, and Regional Specialization of Astrocytes, vol. 1, ( Fedoroff, S; Vernadakis, A, eds.), Academic, New York, pp. 35–74.Google Scholar
  31. Fedoroff S; Vernadakis A (eds) (1986) Astrocytes, vols. 1,2,3. Academic, New York.Google Scholar
  32. Ffrench-Constant CF; Raff MC (1986) Proliferating bipotential progenitor cells in adult rat optic nerve. Nature 319: 494–502.Google Scholar
  33. Frederiksen K; Jat PS; Valtz N; Levy D; McKay R (1988) Immortalization of precursor cells from mammalian CNS. Neuron 1: 439–448.PubMedGoogle Scholar
  34. Fridman R; Fuks Z; Ovadia H; Vlodarsky I (1985) Differential, structural requirements for the induction of cell attachment, proliferation, and differentiation by the extracellular matrix. Exp Cell Res 157: 181–194.PubMedGoogle Scholar
  35. Furukawa S; Furukawa Y; Satoyoshi E; Hayashi K (1986) Synthesis and secretion of nerve growth factor by mouse astroglial cells in culture. Biochem Res Commun 136: 57–63.Google Scholar
  36. Galiana E; Bernard R; Borde I; Rouget P; Evrard C (1993) Proliferation and differentiation properties of bipotential glial progenitor cell lines immortalized with the adenovirus. EIA gene. J Neurosci Res 36: 133–146.PubMedGoogle Scholar
  37. Galileo DS; Gray GE; Owens GC; Majors J; Sanes JR (1990) Neurons and glia arise from a common progenitor in chicken optic tectum: Demonstration with two retroviruses and cell type-specific antibodies. Proc Natl Acad Sci USA 87: 458–462.PubMedGoogle Scholar
  38. Gasser UE; Hatten MR (1990) Neuron—glia interactions of rat hippocampal cells in vitro: Glial-guided neuronal migration and neuronal regulation of glia differentation. J Neurosci 10: 1276–1285.PubMedGoogle Scholar
  39. Ghooray GT; Martin GF (1993) Development of radial glia and astrocytes in the spinal cord of the north american poossum (Dedelphis virginiana) An immunohistochemical study using anti-vimentin and anti-glial fibrillary acidic protein. Glia 9: 1–9.PubMedGoogle Scholar
  40. Gospodarowicz D; Gonzales R; Fujii DK (1983) Are factors originating from serum plasma or cultured cells involved in the growth-promoting effect of the extracellular matrix produced by cultured bovine corneal endothelial cells. J Cell Physiol 114: 191–202.PubMedGoogle Scholar
  41. Grafe MR; Schoenfeld TA (1982) Radial glia in the postnatal olfactory tubercle of hamster. Dev Brain Res 4: 115–118.Google Scholar
  42. Gray GE; Sanes JR (1992) Lineage of radial glia in the chicken optic tectum. Development 114: 271–283.PubMedGoogle Scholar
  43. Hamburger V (1958) Regression vs peripheral control of differentiation in motor hypoplasia. Am J Anat 102: 365–410.PubMedGoogle Scholar
  44. Hamburger V; Levi-Montalcini R (1949) Proliferation, differentiation and degeneration in the spinal ganglia of the chick embryo under normal and experimental conditions. J Exp Zool 111: 457–502.PubMedGoogle Scholar
  45. Han VKM; Smith A; Myint W; Nygard K; Bradshaw S (1992) Mitogenic activity of epidermal growth factor on newborn rat astroglia: Interaction with insulin-like factors.Encodrin 131: 1134–1142.Google Scholar
  46. Hatten ME (1985) Neuronal regulation of astroglial morphology and proliferation in vitro. J Cell Biol 100: 384–396.PubMedGoogle Scholar
  47. Hatten ME (1990) A common mechanism for glia-guided neuronal migration in different regions of the developing mammalian brain. Trends Neurosci 13: 179–184.PubMedGoogle Scholar
  48. Havrankova J; Schmechel D; Roth J; and Brownstein M (1978) Identification of insulin in rat brain. Proc Natl Acad Sci USA 75: 5737–5741.PubMedGoogle Scholar
  49. Hawrot E (1980) Cultured sympathetic neurons: effects of cell-derived and synthetic substrata on survival and development. Dev Biol 74: 136–141.PubMedGoogle Scholar
  50. Hendricks SA; Roth J; Rischi S; Becker KL (1983) Insulin in the nervous system (Krieger, DT; Brownstein, MJ; Martin, JB, eds.), Wiley, New York, pp. 903–939.Google Scholar
  51. Hirano M; Goldman JE (1988) Gliogenesis in rat spinal cord: Evidence for origin of astrocytes and oligodendrocytes from radial precursors. J Neurosci Res 21: 155–167.PubMedGoogle Scholar
  52. Houlgatte R; Mallat M; Brachet P; Prochiantz A (1989) Secretion of nerve growth factor in culture of glial cells and neurons derived from different regions of the mouse brain. J Neurosci Res 24: 143–152.PubMedGoogle Scholar
  53. Huff RK; Schreier WC (1989) Fibroblast growth factor pretreatment reduces epidermal growth factor-induced proliferation in rat astrocytes. Life Sci 45: 1515–1520.PubMedGoogle Scholar
  54. Hunter SF; Bottensten JE (1991) O-2A glia progenitors from mature brain respond to CNS neuronal cell line-derived growth factors. J Neurosci Res 28: 574–582.PubMedGoogle Scholar
  55. Hydén H (1961) Satellite cells in the nervous system. Sci Am 205: 62–70.PubMedGoogle Scholar
  56. Ignacio V; Gansmuller A; Collins VP; Suard I; Jacque C (1990) Short term postgrafting morphological alteration of glia from an adult brain transplant. Glia 3: 140–150.PubMedGoogle Scholar
  57. Jung M; Crany AJ; Blakemor WF; Hoppe D; Kettenmann H; Trotter J (1994) In vitro and in vivo characterization of glial cells immortalized with a temperature sensitive SV40 T antigen-containing retrovirus. J Neurosci Res 37: 182–196.PubMedGoogle Scholar
  58. Kenigsbery RL; Mazzoni IE; Collier B; Cuello AC (1992) Epidermal growth factor affects both glia and cholinergic neurons in septal cell cultures. Neurosci 50: 85–97.Google Scholar
  59. Kentroti S; Vernadakis A (1990) Neuronal plasticity in the developing chick brain: Interaction of ethanol and neuropeptides. Dev Brain Res 56: 205–210.Google Scholar
  60. Kentroti S; Vernadakis A (1992) Ethanol administration during early embryo-genesis affects neuronal phenotypes at a time when neuroblasts are pluripotential. J Neurosci Res 33: 617–625.PubMedGoogle Scholar
  61. Kentroti S; Vernadakis A (1995) Immunocytochemical and biochemical characterizations of glial phenotypes in cultures derived from 3-d-old chick embryo encephalon. Glia, submitted.Google Scholar
  62. Kleinman HK; Cannon FB; Laurie GW; Hassell JR; Aumailley M; Terranova VP; Martin GR; Dubois-Dalca M (1985) Biological activities of laminin. J Cell Biochem 27: 317–325.PubMedGoogle Scholar
  63. Kozlova M; Kentroti S; Vernadakis A (1993) Influence of culture substrata on the differentiation of advanced passage glial cells in cultures from aged mouse cerebral hemispheres. Int J Dev Neurosci 11: 513–519.PubMedGoogle Scholar
  64. Le Douarin NM; Renaud D; Teillet MA; Le Douarin GH (1975) Cholinergic differentiation of presumptive adrenergic neuroblasts in interspecific chimeras after heterotopic transplantations. Proc Nat Acad Sci USA 72: 728–732.PubMedGoogle Scholar
  65. Lee K; Kentroti S; Billie H; Bruce C; Vernadakis A (1992a) Comparative biochemical, morphological, and immocytochemical studies between C-6 glial cells of early and late passages and advanced passages of glial cells derived from aged mouse cerebral hemispheres. Glia 6: 245–257.PubMedGoogle Scholar
  66. Lee K; Kentroti S; Vernadakis A (1992b) Differences in neuronal and glial cell phenotypic expression in neuron—glia co-cultures: Influence of glia-conditioned media and living glial cell substrate. Brain Res Bull 28: 861–870.PubMedGoogle Scholar
  67. Levitt P; Rakic P (1980) Immunoperoxidase localization of glial fibrillary acidic protein in radial glial cells and astrocytes of the developing rhesus monkey brain. J Comp Neurol 193: 417–448.Google Scholar
  68. Lin HH; Connor JR (1989) The development of the transferrin-transferrin receptor system in relation to astrocytes, MBP and galactocerebroside in normal and myelin-deficient rat optic nerves. Dev Brain Res 49: 281–293.Google Scholar
  69. Lois C; Alvarez-Buylla A (1993) Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia. Proc Natl Acad Sci USA 90: 2074–2077.PubMedGoogle Scholar
  70. Loret C; Sensenbrenner M; Labourdette G (1989) Differential phenotypic expression induced in cultured rat astroblasts by acidic fibroblast growth factor, epidermal growth factor and thrombin. J Biol Chem 264: 8319–8327.PubMedGoogle Scholar
  71. Markelonis GJ; Oh TU; Dion TL; Bregman BS; Pugh MA; Royal GM; Kim YC; Hobbs SL (1988) Localization of transferrin within the developing vertebrate nervous system. Rev Neurol (Paris) 144: 648–655.Google Scholar
  72. Mash DC; Pablo J; Flynn DD; Efange SMN; Weiner WJ (1990) Characterization and distribution of transferrin receptors in the rat brain. J Neurochem 55: 1972–1979.PubMedGoogle Scholar
  73. McCarthy GF; Leblond CP (1988) Radioautographic evidence for low astrocyte turnover and modest oligodendrocyte production in the corpus cllosum of adult mice infused with 3H-thymidine. J Comp Neurol 271: 589–603.PubMedGoogle Scholar
  74. Mescher AL; Munaim SI (1988) Transferrin and the growth-promoting effect of nerves. Int Rev Cytol 110: 1–26.PubMedGoogle Scholar
  75. Misson JP; Takahashi T; Caviness VS Jr (1991) Ontogeny of radial and other astroglia cells in murine cerebral cortex. Glia 4: 138–148.Google Scholar
  76. Montiel F; Ortiz Caro J; Villa A; Pascual A; Aranda A (1989) Presence of insulin receptors in culture glial C-6 cells: Regulation by butyrate. Biochem J 258: 147–155.PubMedGoogle Scholar
  77. Muller CM (1992) A role for glial cells in activity-dependent control nervous plasticity: Review and hypothesis. Int Rev Neurobiol 34: 215–221.PubMedGoogle Scholar
  78. Navascués J; Rodriguez-Gallardo L; Martin-Partido G; Alvarez IS (1985) Proliferation of glial precursors during early development of the chick optic nerve. Anat Embryol 172: 365–373.PubMedGoogle Scholar
  79. Norenberg MD; Martinez-Hernandez A (1979) Fine structural localization of glutamine synthetase in astrocytes of rat brain. Brain Res 161: 303–310.PubMedGoogle Scholar
  80. Norton WT; Farooq M (1989) Astrocytes cultured from mature brain derived from glia precursor cells. J Neurosci 9: 769–775.PubMedGoogle Scholar
  81. Olson JA; Shiverick KT; Ogilvie S; Buh WC; Raizada MK (1991) Developmental expression of rat insulin-like growth factor binding protein-2 by astrocyte glial cells in culture. Endocrinology 129: 1066–1074.PubMedGoogle Scholar
  82. Panayotou G; End P; Aumailley M; Timpl R; Engel J (1989) Domains of laminin with growth factor activity. Cell 56: 93–101.PubMedGoogle Scholar
  83. Paetau A; Mellstrom K; Vahers A; Haltia M (1980); Distribution of a major connective tissue protein, fibronectin in normal and neoplastic human nervous tissue. Acta Neuropathol 51: 47–51.PubMedGoogle Scholar
  84. Patterson PH; Chun LLY (1977a) The induction of acetylcholine synthesis in primary cultures of dissociated rat sympathetic neurons: I. Effects of conditioned medium. Dev Biol 56: 263–280.PubMedGoogle Scholar
  85. Patterson PH; Chun LLY (1977b) The induction of acetylcholine synthesis in primary cultures of dissociated rat sympathetic neurons: II. Developmental aspects. Dev Biol 60: 473–481.PubMedGoogle Scholar
  86. Perraud F; Besnard F; Pettman B; Sensenbrenner M; Labourdette G (1988) Effects of acidic and basic fibroblast growth factors (aFGF and bFGF) on the proliferation and glutamine synthetase expression of rat astroblasts in culture. Glia 1: 124–131.PubMedGoogle Scholar
  87. Perraud F; Labourdette G; Eclancher F; Sensenbrenner M (1990); Primary cultures of astrocytes from different brain areas of newborn rats and effects of basic fibroblast growth factor. Dev Neurosci 12: 11–21.PubMedGoogle Scholar
  88. Pixley SR; de Vellis J (1984) Transition between immature radial glia and mature astrocytes studied with monoclonal antibody to vimentin. Dev Brain Res 15: 201–209.Google Scholar
  89. Poduslo SE (1975) The isolation and characterization of a plasma membrane and myelin fraction derived from oligodendroglia of calf brain. J Neurochem 24: 647–664.PubMedGoogle Scholar
  90. Poduslo SE; Norton WT (1972) Isolation and some chemical properties of oligodendroglia from calf brain. J Neurochem 19: 727–736.PubMedGoogle Scholar
  91. Raff MC; Abney ER; Cohen J; Lindsey R; Noble MD (1983) Two types of astrocytes in development rat white matter: differences in morphology, surface gangliosides, and growth characteristics. J Neurosci 3: 1289–1300.PubMedGoogle Scholar
  92. Raff MC; Williams BP; Miller RH (1984) The in vitro differentiation of a bipotential glial progenitor cell. EMBO J 3: 1857–1864.PubMedGoogle Scholar
  93. Raff MC; Mirsky R; Fields KL; Lisak RP; Dorfman SH; Silberberg DH; Gregson WA; Leibowitz S; Kennedy MC (1978) Galactocerebroside is a specific cell-surface antigenic marker for oligodendrocytes in culture. Nature (Lond) 274: 816.Google Scholar
  94. Raizada MD; Yang JW; Fellows RE (1980) Binding of 125 I-insulin to specific receptors and stimulation of nucleotide incorporation in cells cultured from rat brain. Brain Res 200: 389–400.PubMedGoogle Scholar
  95. Reichenback A (1989) Attempt to classify glial cells by means of their process specialization using rabbit Muller cells as an example of cytotopographic specialization of glial cells. Glia 2: 250–259.Google Scholar
  96. Rogers S; Letourneau PS; Palm SL; McCarthy J; Furcht L (1983) Neurite extension by peripheral and central nervous system neurons in response to substratum-bound fibronectin and laminin. Dev Biol 98: 212–220.PubMedGoogle Scholar
  97. Rouget P; Le Bert M; Brodie T; Evrard C (1992) Generation of neuronal cell lines by transfer of viral ongogenes, in Cell Lines in Neurobiology, (Wood, JN, ed.), IRL Oxford University Press, Oxford, pp. 27–54.Google Scholar
  98. Rudge JS; Alderson RF; Pasnikowski E; McClain J; Ip NY; Lindsay RM (1992) Expression of ciliary neurotrophic factor and neurotrophins—Nerve growth factor, brain-derived neurotrophic factor and neurotrophin 3—in cultured rat hippocampal astrocytes. Europ J Neurosci 4: 459–471.Google Scholar
  99. Schiffer D; Giordana MJ; Mighels A; Giaccone G; Pezzotta S; Mauro A (1986) Glial fibrillary acidic protein and vimentin in the experimental glial reaction of the rat brain. Brain Res 374: 110–118.PubMedGoogle Scholar
  100. Schmechel DE; Rakic P (1979) A Golgi study of radial glial cells in developing monkey telencephalon: Morphogenesis and transformation into astrocytes. Anat Embryol 156: 115–152.PubMedGoogle Scholar
  101. Schnitzer J; Franke W; Schachner M (1981) Immunocytochemical demonstration of vimentin in astrocytes and ependymal cells of developing and adult mouse nervous system. J Cell Biol 90: 435–447.PubMedGoogle Scholar
  102. Selmaj K; Shafit-Zagurdo B; Aquino DA; Farooq M; Raine CS; Norton WT; Brossan CF (1991) Tumor necrosis factor-induced proliferation of astrocytes from mature brain is associated with down-regulation of glial fibrillary acidic protein mRNA. J Neurochem 57: 823–830.PubMedGoogle Scholar
  103. Shitara N; Kitamura K; Wado T; Nakamura H; Takakura K (1989) Transferrin receptor molecules in human cell lines derived from the CNS malignant gliomas: Immunohistochemical and flowcytometric study. Acta Histochem Cytochem 22: 275–288.Google Scholar
  104. Skoff RP; Vaughn JE (1971) An autoadiographic study of cellular proliferation in degenerating rat optic nerve. J Comp Neurol 141: 133–156.PubMedGoogle Scholar
  105. Sternberger LA; Handy PH Jr; Cuculis JJ; Meyer HG (1970) The unlabeled antibody-enzyme method of immunocytochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidaseantihorseradish peroxidase) and its use in the identification of spirochetes. J Histochem Cytochem 18: 315–333.PubMedGoogle Scholar
  106. Takamiya Y; Kohsake S; Toyo S; Otani M; Tsukada Y (1988) Immunocytochemical studies on the proliferation of reactive astrocytes and the expression of cytoskeletal proteins following brain injury in rats. Dev Brain Res 38: 201–210.Google Scholar
  107. Tetzlaff W; Graeber MB; Bisby MA; Kreutzberg GW (1988) Increased glia fibrillary acidic protein synthesis in astrocytes during retrograde reaction of the rat facias nucleus. Glia 1: 90–95.PubMedGoogle Scholar
  108. Trotter J; Crang AJ; Schachner M; Blakemore WF (1993) Lines of glial precursor cells immortalized with a temperature-sensitive oncogene give rise to astrocytes and oligodendrocytes following transplantation into demyelinated lesions in the central nervous system. Glia 9: 25–40.PubMedGoogle Scholar
  109. Verndakis A (1988) Neuron—glia interrelations. Int Rev Neurob 30: 149–224.Google Scholar
  110. Vernadakis A; Kentroti S (1994) Glial cells derived from aged mouse brain in culture display both mature and immature astrocytic phenotypes. J Neurosci Res 38: 451–458.PubMedGoogle Scholar
  111. Vernadakis A; Davies D; Sakellaridis N; Mangoura D (1986) Growth patterns of glial cells dissociated from newborn and aged mouse brain with cell passage. J Neurosci Res 13: 79–85.Google Scholar
  112. Vernadakis A; Mangoura D; Sakellaridis N; Linderholm S (1984) Glia cells dissociated from newborn and aged mouse brain. J Neurosci Res 11: 253–262.PubMedGoogle Scholar
  113. Vlodaysky I; Folkman J; Sullivan R; Fridman R; Ishai-Michaeli R; Sasse J; Klagsbrun M (1987) Endothelial cell-derived basic fibroblast growth factor: Synthesis and deposition into subendothelial extracellular matrix. Proc Natl Acad Sci USA 84: 2292–2296.Google Scholar
  114. Voigt T (1989) Development of glial cells in the cerebral wal of ferrets: direct tracing of their transformation from radial glia into astrocytes. J Comp Neurol 289: 74–88.PubMedGoogle Scholar
  115. Wang SL; Shiverick KT; Ogilvie S; Dunn WA; Raizada MK (1989) Characteizations of epidermal growth factor receptors in astrocytic glia, and neuronal cells in primary culture. Endocrinology 124: 240–247.PubMedGoogle Scholar
  116. Weiss P (1961) Guiding principles in cell aggregation. Expl Cell Res (Suppl) 8: 260–281.Google Scholar
  117. Whittmore SR; Neary JT; Kleitman N; Sanon HR; Benigno A; Donahue RP; Norenberg MD (1994) Isolation and characterization of conditionally immortalized astrocyte cell lines derived from adult human spinal cord. Glia 10: 211–226.Google Scholar
  118. Wolinsky EJ; Landis SC; Patterson PH (1985) Expression of noradrenergic and cholinergic traits by sympathetic neurons cultured without serum. J Neurosci 5: 1497–1508.PubMedGoogle Scholar
  119. Wolswijk G; Munro PMG; Riddle PN; Noble M (1991) Orgin, growth factor responses, and ultrastructural characteristics of an adult-specific glial progenitor cell, in Glia-Neuronal Interactions (Abbott, NJ, ed. ), Ann NY Acad Sci 663: 502–504.Google Scholar
  120. Yamakumi T; Ozawa F; Hashimoto F; Kuwano R; Takahasi Y; Amano T (1987) Expression of fl-nerve growth factor mRNA in rat glioma cells and astrocytes from rat brain. FEBS Lett. 223: 117–121.Google Scholar
  121. Yen SH; Fields KL (1981) Antibodies to neurofilament, glial filament and fibroblast intermediate filament proteins bind to different cell types of the nervous system. J Cell Biol 88: 115–126.PubMedGoogle Scholar
  122. Yoshida K; Gage FH (1991) Fibroblast growth factors stimulate nerve growth factor synthesis and secretion by astrocytes. Brain Res 538: 118–126.PubMedGoogle Scholar

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© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Antonia Vernadakis
  • Susan Kentroti

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