Principles of Embedding and Common Protocols

  • Yuehuei H. An
  • Patricia L. Moreira
  • Qian K. Kang
  • Helen E. Gruber
Chapter

Abstract

Embedding techniques were first developed in the mid 1800s in response to the significant improvements in light microscopy. As the resolution of microscopy increased, so did the need for improved quality of the tissue specimens to be analyzed. The specimens needed to be cut in much thinner slices, which could only be done if embedded in a suitable medium supporting the material and providing the hardness required for thinner sectioning. Edwin Klebs introduced the paraffin-embedding methodology in 1869.18 However, at that time, the material was not completely infiltrated with paraffin and the sections were cut freehand with a razor.

Keywords

Hard Tissue Benzoyl Peroxide Methyl Salicylate Bone Specimen Ground Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Yuehuei H. An
    • 1
  • Patricia L. Moreira
    • 1
  • Qian K. Kang
    • 1
  • Helen E. Gruber
    • 2
  1. 1.Orthopaedic Research LaboratoryMedical University of South CarolinaCharlestonUSA
  2. 2.Orthopaedic Research BiologyCarolinas Medical CenterCharlotteUSA

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