Monitoring Intracellular Oxygen Concentration: Implications for Hypoxia Studies and Real-Time Oxygen Monitoring

  • Michelle Potter
  • Luned Badder
  • Yvette Hoade
  • Iain G. Johnston
  • Karl J. Morten
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 876)

Abstract

The metabolic properties of cancer cells have been widely accepted as a hallmark of cancer for a number of years and have shown to be of critical importance in tumour development. It is generally accepted that tumour cells exhibit a more glycolytic phenotype than normal cells. In this study, we investigate the bioenergetic phenotype of two widely used cancer cell lines, RD and U87MG, by monitoring intracellular oxygen concentrations using phosphorescent Pt-porphyrin based intracellular probes. Our study demonstrates that cancer cell lines do not always exhibit an exclusively glycolytic phenotype. RD demonstrates a reliance on oxidative phosphorylation whilst U87MG display a more glycolytic phenotype. Using the intracellular oxygen sensing probe we generate an immediate readout of intracellular oxygen levels, with the glycolytic lines reflecting the oxygen concentration of the environment, and cells with an oxidative phenotype having significantly lower levels of intracellular oxygen. Inhibition of oxygen consumption in lines with high oxygen consumption increases intracellular oxygen levels towards environmental levels. We conclude that the use of intracellular oxygen probes provides a quantitative assessment of intracellular oxygen levels, allowing the manipulation of cellular bioenergetics to be studied in real time.

Keywords

Intracellular oxygen Mitochondria Oxidative phosphorylation Oxygen probe Aerobic glycolysis 

Copyright information

© Springer Science+Business Media, New York 2016

Authors and Affiliations

  • Michelle Potter
    • 1
  • Luned Badder
    • 2
  • Yvette Hoade
    • 1
  • Iain G. Johnston
    • 3
  • Karl J. Morten
    • 1
  1. 1.Nuffield Department of Obstetrics and GynaecologyUniversity of OxfordOxfordUK
  2. 2.European Cancer Stem Cell Research Institute, University of CardiffCardiffUK
  3. 3.Department of MathematicsImperial College LondonLondonUK

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