Metabolomics and Secondary Metabolite Profiling of Filamentous Fungi

  • Bernhard Kluger
  • Sylvia Lehner
  • Rainer Schuhmacher
Chapter
First Online:
Part of the Fungal Biology book series (FUNGBIO)

Abstract

Driven by significant technical developments in analytical instrumentation and the tremendous advances in biological sciences, a change in paradigm from reductionist to holistic approaches for the study of filamentous fungi can be observed currently. This development is also reflected by the emergence of metabolomics as the latest of the so-called -omics disciplines. Metabolomics , the scientific discipline dealing with the determination of the low-molecular-weight complement of biological systems is increasingly being used to investigate the biochemical composition of fungi and their biological interactions. This chapter introduces the general concept of metabolomics and summarizes the analytical approaches used for the study of fungal exo- and endo-metabolomes. Current applications in fungal metabolomics and metabolite profiling such as chemotaxonomical classification, the search and production of novel beneficial secondary metabolites as well the dissection of host–fungus interactions are presented. Finally, novel emerging approaches for the improved fungal metabolomics, such as the use of stable isotope labeled biological samples and tracer metabolites and novel techniques, that enable spatial and temporal dissection of metabolite production, are briefly summarized.

Keywords

Liquid chromatography-mass spectrometry (LC-MS) Gas chromatography-mass spectrometry (GC-MS) Untargeted metabolite profiling Targeted metabolite profiling Exometabolome Endometabolome Chemotaxonomy Secondary metabolites Host–fungus interactions 
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Notes

Acknowledgements

The authors would like to thank the Austrian Science Fund (project SFB Fusarium 3706-B11) for financial support. Thanks are also offered to Benedikt Warth for his valuable comments on the draft of this manuscript as well as Maria Doppler and Christoph Bueschl for their kind assistance in preparing the figures. The presented work contributes in part to the PhD thesis of Bernhard Kluger.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Bernhard Kluger
    • 1
  • Sylvia Lehner
    • 1
  • Rainer Schuhmacher
    • 1
  1. 1.Department of Agrobiotechnology (IFA-Tulln), Center for Analytical ChemistryUniversity of Natural Resources and Life SciencesTullnAustria

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