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Structural Analysis of Oligosaccharides and Glycoconjugates Using NMR

  • Yoshiki Yamaguchi
  • Takumi Yamaguchi
  • Koichi Kato
Chapter
Part of the Advances in Neurobiology book series (NEUROBIOL, volume 9)

Abstract

Carbohydrate chains play critical roles in cellular recognition and subsequent signal transduction in the nervous system. Furthermore, gangliosides are targets for various amyloidogenic proteins associated with neurodegenerative disorders. To better understand the molecular mechanisms underlying these biological phenomena, atomic views are essential to delineate dynamic biomolecular interactions. Nuclear magnetic resonance (NMR) spectroscopy provides powerful tools for studying structures, dynamics, and interactions of biomolecules at the atomic level. This chapter describes the basics of solution NMR techniques and their applications to the analysis of 3D structures and interactions of glycoconjugates in the nervous system.

Keywords

Nuclear magnetic resonance Structure Dynamics Interaction Oligosaccharide Glycolipid 

Abbreviations

Amyloid β

DIS

Deuterium-induced isotope shift

FID

Free induction decay

HSQC

Heteronuclear single-quantum coherence

MD

Molecular dynamics

NMR

Nuclear magnetic resonance

NOE

Nuclear Overhauser effect

NOESY

NOE spectroscopy

PCS

Pseudocontact shift

PRE

Paramagnetic relaxation enhancement

REMD

Replica exchange MD

RF

Radio frequency

STD

Saturation transfer difference

TRNOE

Transferred NOE

TROSY

Transverse relaxation optimized spectroscopy

Notes

Acknowledgments

This study was partly supported by JSPS/MEXT KAKENHI Grant-in-Aid for Scientific Research on Innovation Areas (20107004 and 25102008), Scientific Research (A) (24249002), Scientific Research (C) (25460054), Challenging Exploratory, Research (26560451), and Young Scientists (B) (24750170).

Compliance with Ethics Requirements The authors declare that they have no conflict of interest and that they have used no human subjects in work cited that was done in their laboratory.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Structural Glycobiology Team, Systems Glycobiology Research GroupRIKEN-Max Planck Joint Research Center, RIKEN Global Research ClusterWako-City, SaitamaJapan
  2. 2.Institute for Molecular Science and Okazaki Institute for Integrative BioscienceMyodaiji, OkazakiJapan
  3. 3.Graduate School of Pharmaceutical SciencesNagoya City UniversityMizuho-ku, NagoyaJapan
  4. 4.The Glycoscience InstituteOchanomizu UniversityBunkyo-kuJapan

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