Schistosomatoidea and Diplostomoidea

  • Petr Horák
  • Libuše Kolářová
  • Libor Mikeš
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 766)


Trematodes of the order Diplostomida are well known as serious pathogens of man, and both farm and wild animals; members of the genus Schistosoma (Schistosomatidae) are responsible for human schistosomiasis affecting more than 200 million people in tropical and subtropical countries, and infections of mammals and birds by animal schistosomes are of great veterinary importance. The order Diplostomida is also rich in species parasitizing other major taxa of vertebrates. The Aporocotylidae are pathogenic in fish and Spirorchiidae in reptiles. All these flukes have two-host life cycles, with asexually reproducing larvae usually in molluscs and occasionally in annelids, and adults usually live in the blood vessels of their vertebrate hosts. Pathology is mostly associated with inflammatory reactions to eggs trapped in various tissues/organs. On the other hand, the representatives of Diplostomidae and Strigeidae have three- or four-host life cycles in which vertebrates often serve not only as definitive, but also as intermediate or paratenic hosts. Pathology is usually associated with migration of metacercariae and mesocercariae within the host tissues. The impact of these trematode infections on both farm and wild animals may be significant.


Intermediate Host Adult Worm Definitive Host Paratenic Host Intermediate Snail Host 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Recent research of the authors has been supported by the following institutions and foundations: Charles University in Prague (PRVOUK Nos. P25/LF1/2 and P41/PřF; UNCE No. 204017/2012; SVV Nos. 260026/2014 and 260074/2014), Czech Science Foundation (project No. 13-29577S), and Grant Agency of the Ministry of Health of CR (project No. NT 13108–4/2012). We appreciate helpfulness of our colleagues (Dr. J. Bulantová, Prof. I. Dyková, Dr. L. Lichtenbergová, Dr. M. Ondračková, Dr. M. Soldánová) who provided photos of helminths mentioned in our chapter.


  1. 1.
    Olson PD, Cribb TH, Tkach VV et al (2003) Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). Int J Parasitol 33:733–755PubMedGoogle Scholar
  2. 2.
    Kalbe M, Haberl B, Haas W (1997) Miracidial host-finding in Fasciola hepatica and Trichobilharzia ocellata is stimulated by species-specific glycoconjugates released from the host snails. Parasitol Res 83:806–812PubMedGoogle Scholar
  3. 3.
    Haas W (2003) Parasitic worms: strategies of host finding, recognition and invasion. Zoology (Jena) 106:349–364Google Scholar
  4. 4.
    Horák P, Kolářová L (2005) Molluscan and vertebrate immune responses to bird schistosomes. Parasite Immunol 27:247–255PubMedGoogle Scholar
  5. 5.
    De Jong-Brink M, Bergamin-Sassen M, Solis Soto M (2001) Multiple strategies of schistosomes to meet their requirements in the intermediate snail host. Parasitology 123:S129–S141PubMedGoogle Scholar
  6. 6.
    Kašný M, Mikeš L, Dolečková K et al (2011) Cathepsins B1 and B2 of Trichobilharzia spp., bird schistosomes causing cercarial dermatitis. In: Robinson MW, Dalton JP (eds) Cysteine proteases of pathogenic organisms. Landes Bioscience, AustinGoogle Scholar
  7. 7.
    Horák P, Kovář L, Kolářová L et al (1998) Cercaria-schistosomulum surface transformation of Trichobilharzia szidati and its putative immunological impact. Parasitology 116:139–147PubMedGoogle Scholar
  8. 8.
    Haas W, Van de Roemer A (1998) Invasion of the vertebrate skin by cercariae of Trichobilharzia ocellata: penetration processes and stimulating host signals. Parasitol Res 84:787–795PubMedGoogle Scholar
  9. 9.
    Horák P, Kolářová L (2011) Snails, waterfowl and cercarial dermatitis. Freshwater Biol 56:779–790Google Scholar
  10. 10.
    Skírnisson K, Aldhoun JA, Kolářová L (2009) A review on swimmer’s itch and the occurrence of bird schistosomes in Iceland. J Helminthol 83:165–171PubMedGoogle Scholar
  11. 11.
    Soleng A, Mehl R (2011) Geographical distribution of cercarial dermatitis in Norway. J Helminthol 85:345–352PubMedGoogle Scholar
  12. 12.
    Brant SV, Loker ES (2009) Molecular systematics of the avian schistosome genus Trichobilharzia (Trematoda: Schistosomatidae) in North America. J Parasitol 95:941–963PubMedCentralPubMedGoogle Scholar
  13. 13.
    Davis NE (2006) A survey of waterfowl for echinostomes and schistosomes from Lake Wanaka and the Waitaki River watershed, New Zealand. J Helminthol 80:33–40PubMedGoogle Scholar
  14. 14.
    Larsen AH, Bresciani J, Buchmann K (2004) Increasing frequency of cercarial dermatitis at higher latitudes. Acta Parasitol 49:217–221Google Scholar
  15. 15.
    Beer SA, Voronin MV (2007) Cercariases in urban ecosystems. Nauka, MoscowGoogle Scholar
  16. 16.
    Chanová M, Vuong S, Horák P (2007) Trichobilharzia szidati: the lung phase of migration within avian and mammalian hosts. Parasitol Res 100:1243–1247PubMedGoogle Scholar
  17. 17.
    Horák P, Kolářová L, Adema CM (2002) Biology of the schistosome genus Trichobilharzia. Adv Parasitol 52:155–233PubMedGoogle Scholar
  18. 18.
    Horák P, Dvořák J, Kolářová L et al (1999) Trichobilharzia regenti, a pathogen of the avian and mammalian central nervous system. Parasitology 119:577–581PubMedGoogle Scholar
  19. 19.
    Lichtenbergová L, Lassmann H, Jones M et al (2011) Trichobilharzia regenti: host immune response in the pathogenesis of neuroinfection in mice. Exp Parasitol 128:328–335PubMedGoogle Scholar
  20. 20.
    Kolářová L, Horák P, Čada F (2001) Histopathology of CNS and nasal infections caused by Trichobilharzia regenti in vertebrates. Parasitol Res 87:644–650PubMedGoogle Scholar
  21. 21.
    Chanová M, Horák P (2007) Terminal phase of bird schistosomiasis caused by Trichobilharzia regenti (Schistosomatidae) in ducks (Anas platyrhynchos f. domestica). Folia Parasitol 54:105–107PubMedGoogle Scholar
  22. 22.
    Kolářová L, Horák P, Skírnisson K et al (2013) Cercarial dermatitis, a neglected allergic disease. Clin Rev Allergy Immunol 45:63–74.PubMedGoogle Scholar
  23. 23.
    Horák P, Kolářová L (2001) Bird schistosomes—do they die in mammalian skin? Trends Parasitol 17:66–69PubMedGoogle Scholar
  24. 24.
    Horák P, Mikeš L, Rudolfová J et al (2008) Penetration of Trichobilharzia cercariae into mammals: dangerous or negligible event? Parasite 15:299–303PubMedGoogle Scholar
  25. 25.
    Soldánová M, Selbach C, Kalbe M et al (2013) Swimmer’s itch: etiology, impact, and risk factors in Europe. Trends Parasitol 29:65–74PubMedGoogle Scholar
  26. 26.
    Kolářová L, Horák P, Skírnisson K (2010) Methodical approaches in the identification of areas with a potential risk of infection by bird schistosomes causing cercarial dermatitis. J Helminthol 84:327–335PubMedGoogle Scholar
  27. 27.
    Horák P, Schets FM, Kolářová L et al (2012) Trichobilharzia. In: Liu D (ed) Molecular detection of human parasitic pathogens. CRC, Boca RatonGoogle Scholar
  28. 28.
    Wulff C, Haeberlein S, Haas W (2007) Cream formulation protecting against cercarial dermatitis by Trichobilharzia. Parasitol Res 101:91–97PubMedGoogle Scholar
  29. 29.
    Devkota R, Brant SV, Thapa A et al (2014) Sharing schistosomes: the elephant schistosome Bivitellobilharzia nairi also infects the greater one-horned rhinoceros (Rhinoceros unicornis) in Chitwan National Park, Nepal. J Helminthol 88:32–40Google Scholar
  30. 30.
    Vimalraj PG, Jayathangaraj MG, Sridhar R et al (2012) Elephant schistosome (Bivitellobilharzia nairi) in free-ranging Asian elephants (Elephas maximus) of Sathyamangalam forest division of Tamil Nadu State. J Vet Parasitol 26:80–81Google Scholar
  31. 31.
    Kinsella JM, Deem SL, Blake S (2004) Endoparasites of African forest elephants (Loxodonta africana cyclotis) from the Republic of Congo and Central African Republic. Comp Parasitol 71:104–110Google Scholar
  32. 32.
    Agatsuma T, Rajapakse RPVJ, Kuruwita VY (2004) Molecular taxonomic position of the elephant schistosome, Bivitellobilharzia nairi, newly discovered in Sri Lanka. Parasitol Int 53:69–75PubMedGoogle Scholar
  33. 33.
    Dvořák J, Mashiyama ST, Braschi S et al (2008) Differential use of protease families for invasion by schistosome cercariae. Biochimie 90:345–358PubMedGoogle Scholar
  34. 34.
    Basch PF, Gupta BC (1988) Immunocytochemical localization of regulatory peptides in six species of trematode parasites. Comp Biochem Physiol C 91:565–570PubMedGoogle Scholar
  35. 35.
    Raiczyk GB, Hall JC (1988) Schistosomatium douthitti: biochemical and morphological effects of an experimental infection in mice. Exp Parasitol 65:187–201PubMedGoogle Scholar
  36. 36.
    Schwanz LE (2006) Schistosome infection in deer mice (Peromyscus maniculatus): impacts on host physiology, behavior and energetics. J Exp Biol 209:5029–5037PubMedGoogle Scholar
  37. 37.
    Stone RH, Frontera-Acevedo K, Saba CF et al (2011) Lymphosarcoma associated with Heterobilharzia americana infection in a dog. J Vet Diagn Invest 23:1065–1070PubMedGoogle Scholar
  38. 38.
    Corapi WV, Ajithdoss DK, Snowden KF et al (2011) Multi-organ involvement of Heterobilharzia americana infection in a dog presented for systemic mineralization. J Vet Diagn Invest 23:826–831PubMedGoogle Scholar
  39. 39.
    Flowers JR, Hammerberg B, Wood SL et al (2002) Heterobilharzia americana infection in a dog. J Am Vet Med Assoc 220:193–196PubMedGoogle Scholar
  40. 40.
    Corapi WV, Birch SM, Carlson KL et al (2011) Heterobilharzia americana infection as a cause of hepatic parasitic granulomas in a horse. J Am Vet Med Assoc 239:1117–1122PubMedGoogle Scholar
  41. 41.
    Corapi WV, Snowden KF, Rodrigues A et al (2012) Natural Heterobilharzia americana infection in horses in Texas. Vet Pathol 49:552–556PubMedGoogle Scholar
  42. 42.
    Standley CJ, Mugisha L, Dobson AP et al (2012) Zoonotic schistosomiasis in non-human primates: past, present and future activities at the human–wildlife interface in Africa. J Helminthol 86:131–140PubMedGoogle Scholar
  43. 43.
    Aldhoun JA, Littlewood DTJ (2012) Orientobilharzia Dutt & Srivastava, 1955 (Trematoda: Schistosomatidae), a junior synonym of Schistosoma Weinland, 1858. Syst Parasitol 82:81–88PubMedGoogle Scholar
  44. 44.
    Singh A, Singh A, Chaudhri SS (2004) Visceral schistosomiasis of domestic animals in India: humoral immune status of infected cattle, sheep and goats against major polypeptide antigens of Schistosoma indicum and S. spindale. Parasite Immunol 26:167–175PubMedGoogle Scholar
  45. 45.
    Wang CR, Chen J, Zhao JP et al (2009) Orientobilharzia species: neglected parasitic zoonotic agents. Acta Trop 109:171–175PubMedGoogle Scholar
  46. 46.
    Arfaa F, Sabaghian H, Ale-Dawood H (1965) Studies on Ornithobilharzia turkestanicum (Skrjabin, 1913) Price, 1929 in Iran. Ann Parasitol Hum Comp 40:45–50PubMedGoogle Scholar
  47. 47.
    Majoros G, Dán Á, Erdélyi K (2010) A natural focus of the blood fluke Orientobilharzia turkestanica (Skrjabin, 1913) (Trematoda: Schistosomatidae) in red deer (Cervus elaphus) in Hungary. Vet Parasitol 170:218–223PubMedGoogle Scholar
  48. 48.
    Lawton SP, Majoros G (2013) A foreign invader or a reclusive native? DNA bar coding reveals a distinct European lineage of the zoonotic parasite Schistosoma turkestanicum (syn. Orientobilharzia turkestanicum (Dutt and Srivastava, 1955)). Infect Genet Evol 14:186–193PubMedGoogle Scholar
  49. 49.
    Sahba GH, Malek EA (1979) Dermatitis caused by cercariae of Orientobilharzia turkestanicum in the Caspian Sea area of Iran. Am J Trop Med Hyg 28:912–913PubMedGoogle Scholar
  50. 50.
    Liu Z, Zhao X, Niu SF (1976) A survey of the etiological agent of rice-field dermatitis with studies on the life-cycle of Orientobilharzia turkestanica var. tuberculata in Jinlin province. Acta Zoolog Sin 22:279–287Google Scholar
  51. 51.
    Shakarbaev E, Haberl B, Loy C et al (2001) Recognition of cattle skin by cercariae of Orientobilharzia turkestanica. Parasitol Res 87:705–707PubMedGoogle Scholar
  52. 52.
    Sumanth S, D’Souza PE, Jagannath MS (2004) A study of nasal an visceral schistosomosis in cattle slaughtered at an abattoir in Bangalore, South India. Rev Sci Tech 23:937–942PubMedGoogle Scholar
  53. 53.
    Haas W, Granzer M, Brockelman CR (1990) Finding and recognition of the bovine host by the cercariae of Schistosoma spindale. Parasitol Res 76:343–350PubMedGoogle Scholar
  54. 54.
    Fransen J, De Bont J, Vercruysse J et al (1990) Pathology of natural infections of Schistosoma spindale Montgomery, 1906, in cattle. J Comp Pathol 103:447–455PubMedGoogle Scholar
  55. 55.
    De Bont J, Van Aken D, Vercruysse J et al (1989) The prevalence and pathology of Schistosoma nasale Rao, 1933 in cattle in Sri Lanka. Parasitology 98:197–202PubMedGoogle Scholar
  56. 56.
    Ravindran R, Kumar A (2012) Nasal schistosomiasis among large ruminants in Wayanad, India. Southeast Asian J Trop Med Public Health 43:586–588PubMedGoogle Scholar
  57. 57.
    Dutt SC (1967) Studies on Schistosoma nasale Rao, 1933. I. Morphology of the adults, egg and larval stages. Indian J Vet Sci 37:249–262Google Scholar
  58. 58.
    Sapate PP, Bhilegaonkar NG, Maske DK (2001) Development of Schistosoma nasale in hamster and mastomys and their pathogenicity. Indian Vet J 78:14–17Google Scholar
  59. 59.
    Sahay MN, Sahai BN (1978) Studies on the susceptibility of the laboratory animals, kids, and lambs to experimental infection with Schistosoma nasale Rao 1933. J Parasitol 64:1135–1136Google Scholar
  60. 60.
    Sumanth S, D'Souza PE, Jagannath MS (2004) Serodiagnosis of nasal and visceral schistosomosis in cattle by counter current immuno electrophoresis. Vet Arhiv 74:427–433Google Scholar
  61. 61.
    Sreenivasa Murthy GS, D'Souza PE, Shrikrishna Isloor K (2013) Evaluation of a polyclonal antibody based sandwich ELISA for the detection of faecal antigens in Schistosoma spindale infection in bovines. J Parasit Dis 37:47–51PubMedCentralPubMedGoogle Scholar
  62. 62.
    Rahman SA, Sano M, Jagannath MS et al (1988) Efficacy of praziquantel against Schistosoma nasale infection in cattle. Trop Anim Health Prod 20:19–22PubMedGoogle Scholar
  63. 63.
    Singh BK, Agrawal MC (2005) Triclabendazole on bovine hepatic schistosomosis. Indian J Anim Sci 75:654–655Google Scholar
  64. 64.
    Moné H, Mouahid G, Morand S (1999) The distribution of Schistosoma bovis Sonsino, 1876 in relation to intermediate host mollusc-parasite relationships. Adv Parasitol 44:99–138PubMedGoogle Scholar
  65. 65.
    De la Torre-Escudero E, Manzano-Román R, Pérez-Sánchez R (2012) Molecular cloning, characterization and diagnostic performance of the Schistosoma bovis 22.6 antigen. Vet Parasitol 190:530–540PubMedGoogle Scholar
  66. 66.
    Majid AA, Marshall TF, Hussein MF et al (1980) Observations on cattle schistosomiasis in the Sudan, a study in comparative medicine. I. Epizootiological observations on Schistosoma bovis in the White Nile province. Am J Trop Med Hyg 29:435–441PubMedGoogle Scholar
  67. 67.
    Makundi AE, Kassuku AA, Maselle RM et al (1998) Distribution, prevalence and intensity of Schistosoma bovis infection in cattle in Iringa district, Tanzania. Vet Parasitol 75:59–69PubMedGoogle Scholar
  68. 68.
    Ferreras-Estrada MC, García-Iglesias MJ, Pérez-Martínez C et al (1998) A pathological study of experimental long-standing Schistosoma bovis infection in sheep. J Comp Pathol 119:479–484PubMedGoogle Scholar
  69. 69.
    De la Torre-Escudero E, Manzano-Román R, Pérez-Sánchez R et al (2010) Cloning and characterization of a plasminogen-binding surface-associated enolase from Schistosoma bovis. Vet Parasitol 173:76–84PubMedGoogle Scholar
  70. 70.
    De la Torre-Escudero E, Manzano-Román R, Siles-Lucas M et al (2012) Molecular and functional characterization of a Schistosoma bovis annexin: fibrinolytic and anticoagulant activity. Vet Parasitol 184:2–36Google Scholar
  71. 71.
    De la Torre-Escudero E, Manzano-Román R, Valero L et al (2011) Comparative proteomic analysis of Fasciola hepatica juveniles and Schistosoma bovis schistosomula. J Proteomics 74:1534–1544PubMedGoogle Scholar
  72. 72.
    Boulanger D, Schneider D, Chapeaux J-P et al (1999) Schistosoma bovis: vaccine effects of a recombinant homologous glutathione S-transferase in sheep. Int J Parasitol 29:415–418PubMedGoogle Scholar
  73. 73.
    Johansen MV, Monrad J, Christensen NØ (1996) Effects of praziquantel on experimental Schistosoma bovis infection in goats. Vet Parasitol 62:83–91PubMedGoogle Scholar
  74. 74.
    Monrad J, Sörén K, Johansen MV et al (2006) Treatment efficacy and regulatory host responses in chronic experimental Schistosoma bovis infections in goats. Parasitology 133:151–158PubMedGoogle Scholar
  75. 75.
    Von Graff L (1907) Das Schmarotzertum im Tierreich und seine Bedeutung für die Artbildung. von Quelle & Meyer, LeipzigGoogle Scholar
  76. 76.
    Yamaguti S (1971) Synopsis of digenetic trematodes of vertebrates. Keigaku, TokyoGoogle Scholar
  77. 77.
    Stunkard HW (1983) The marine cercariae of the Woods Hole, Massachusetts region, a review and revision. Biol Bull 164:14–162Google Scholar
  78. 78.
    Overstreet RM, Køie M (1989) Pearsonellum corventum, gen. et sp. nov. (Digenea: Sanguinicolidae), in serranid fishes from the Capricornia section of the Great Barrier Reef. Aust J Zool 37:71–79Google Scholar
  79. 79.
    Smith JW (2002) Family Sanguinicolidae von Graff, 1907. In: Gibson DI, Jones A, Bray RA (eds) Keys to the Trematoda, vol 1. CAB International/The Natural History Museum, LondonGoogle Scholar
  80. 80.
    Bullard SA, Jensen K, Overstreet RM (2009) Historical account of the two family-group names in use for the single accepted family comprising the “fish blood flukes”. Acta Parasitol 54:78–84Google Scholar
  81. 81.
    Cribb TH, Bray RA (2011) Trematode families and genera: have we found them all? Trends Parasitol 27:149–154PubMedGoogle Scholar
  82. 82.
    Nolan MJ, Cribb TH (2006) Cardicola Short, 1953 and Braya n. gen. (Digenea: Sanguinicolidae) from five families of tropical Indo-Pacific fishes. Zootaxa 1265:1–80Google Scholar
  83. 83.
    Smith JW (1972) The blood flukes (Digenea: Sanguinicolidae and Spirorchidae) of cold-blooded vertebrates and some comparison with the schistosomes. Helminthol Abstr Ser A 41:161–204Google Scholar
  84. 84.
    Smith JW (1997) The blood flukes (Digenea: Sanguinicolidae and Spirorchidae) of cold-blooded vertebrates: Part 1. A review of the published literature since 1971, and bibliography. Helminthol Abstr 66:255–294Google Scholar
  85. 85.
    Smith JW (1997) The blood flukes (Digenea: Sanguinicolidae and Spirorchidae) of cold-blooded vertebrates: Part 2. Appendix I: comprehensive parasite-host list; Appendix II: comprehensive host-parasite list. Helminthol Abstr 66:329–344Google Scholar
  86. 86.
    Cribb TH, Adlard RD, Hayward CJ et al (2011) The life cycle of Cardicola forsteri (Trematoda: Aporocotylidae), a pathogen of ranched southern bluefin tuna, Thunnus maccoyii. Int J Parasitol 41:861–870PubMedGoogle Scholar
  87. 87.
    McMichael-Phillips DF, Lewis JW, Thorndyke MC (1992) Ultrastructure of the egg of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae). J Nat History 26:895–904Google Scholar
  88. 88.
    Kirk RS, Lewis JW (1993) The life-cycle and morphology of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae). Syst Parasitol 25:125–133Google Scholar
  89. 89.
    Cribb TH, Bray RA, Littlewood DTJ (2001) The nature and evolution of the association among digeneans, molluscs and fishes. Int J Parasitol 31:997–1011PubMedGoogle Scholar
  90. 90.
    Køie M (1982) The redia, cercaria and early stages of Aporocotyle simplex Odhner, 1900 (Sanguinicolidae)—a digenetic trematode which has a polychaete annelid as the only intermediate host. Ophelia 21:115–145Google Scholar
  91. 91.
    Køie M, Petersen ME (1988) A new annelid intermediate host (Lanassa nordenskioeldi Malmgren, 1866) (Polychaeta: Terebellidae) for Aporocotyle sp. and a new final host family (Pisces: Bothidae) for Aporocotyle simplex Odhner, 1900 (Digenea: Sanguinicolidae). J Parasitol 74:499–502Google Scholar
  92. 92.
    Gilardoni C, Posadas G, Kroeck MA et al (2011) Monorchiid and aporocotylid cercariae (Digenea) parasitising the purple clam Amiantis purpurata (Bivalvia, Veneridae) from the Southwestern Atlantic coast. Acta Parasitol 56:385–391Google Scholar
  93. 93.
    Martin S, Vazquez R (1984) Biology and behaviour of the cercariae of a Sanguinicola sp. in the river Cilloruelo (Salamanca, Spain). Ann Parasitol Hum Comp 59:231–236PubMedGoogle Scholar
  94. 94.
    Erickson DG, Wallace FG (1959) Studies on blood flukes of the genus Sanguinicola. J Parasitol 45:310–322PubMedGoogle Scholar
  95. 95.
    Meade TG (1967) Life history studies on Cardicola klamathensis (Wales, 1958) Meade and Pratt, 1965 (Trematoda: Sanguinicolidae). Proc Helminthol Soc Wash 34:210–212Google Scholar
  96. 96.
    Sommerville C, Iqbal NAM (1991) The process of infection, migration, growth and development of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae) in carp, Cyprinus carpio. J Fish Dis 14:211–219Google Scholar
  97. 97.
    McLaren DJ, Hockley DJ (1977) Blood flukes have a double outer membrane. Nature 269:147–149PubMedGoogle Scholar
  98. 98.
    Kirk RS, Lewis JW (1996) Migration and development of the blood fluke Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae) in carp Cyprinus carpio L. Parasitology 113:279–285Google Scholar
  99. 99.
    Bray RA, Cribb TH, Littlewood DTJ (2012) Sasala nolani gen. n., sp n. (Digenea: Aporocotylidae) from the body-cavity of the guineafowl puffer fish Arothron meleagris (Lacepede) (Tetraodontiformes: Tetraodontidae) from off Moorea, French Polynesia. Zootaxa 3334:29–41Google Scholar
  100. 100.
    Aiken HM, Bott NJ, Mladineo I et al (2007) Molecular evidence for cosmopolitan distribution of platyhelminth parasites of tunas (Thunnus spp.). Fish Fisheries 8:167–180Google Scholar
  101. 101.
    Aiken HM, Hayward CJ, Nowak BF (2006) An epizootic and its decline of a blood fluke, Cardicola forsteri, in farmed southern bluefin tuna, Thunnus maccoyii. Aquaculture 254:40–45Google Scholar
  102. 102.
    Padrós F, Zarza C, Crespo S (2001) Histopathology of cultured seabream Sparus aurata infected with sanguinicolid trematodes. Dis Aquat Organ 44:47–52PubMedGoogle Scholar
  103. 103.
    McVay MJ, Bakenhaster MD, Bullard SA (2011) Cardicola laruei Short, 1953 (Digenea: Aporocotylidae) from heart of seatrouts, Cynoscion spp. (Perciformes: Sciaenidae) in the Gulf of Mexico and Atlantic Ocean: taxonomic redescription, first observations of egg and miracidium, and comments on geographic distribution and host specificity. Comp Parasitol 78:291–305Google Scholar
  104. 104.
    Ogawa K, Hattori K, Hatai K et al (1989) Histopathology of cultured marine fish, Seriola purpurascens (Carangidae) infected with Paradeontacylix spp. (Trematoda: Sanguinicolidae) in its vascular system. Fish Pathol 28:75–81Google Scholar
  105. 105.
    Faltýnková A, Niewiadomska K, Santos MJ et al (2007) Furcocercous cercariae (Trematoda) from freshwater snails in central Finland. Acta Parasitol 52:310–317Google Scholar
  106. 106.
    Bullard SA, Overstreet RM (2002) Potential pathological effects of blood flukes (Digenea: Sanguinicolidae) on pen-reared marine fishes. In: Proceedings of the 53rd Gulf Caribbean Fisheries InstituteGoogle Scholar
  107. 107.
    Kirk RS (2012) Sanguinicola inermis and related species. In: Woo PTK, Buchmann K (eds) Fish parasites: pathobiology and protection. CAB International, LondonGoogle Scholar
  108. 108.
    Kirk PS, Lewis JW (1992) The laboratory maintenance of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae). Parasitology 104:121–127PubMedGoogle Scholar
  109. 109.
    Alama-Bermejo G, Montero FE, Raga JA et al (2011) Skoulekia meningialis n. gen., n. sp. (Digenea: Aporocotylidae Odhner, 1912), a parasite surrounding the brain of the Mediterranean common two-banded seabream Diplodus vulgaris (Geoffroy Sain-Hilaire, 1817) (Teleostei: Sparidae): description, molecular phylogeny, habitat and pathology. Parasitol Int 60:34–44PubMedGoogle Scholar
  110. 110.
    Cribb TH, Bray RA (2010) Gut wash, body soak, blender and heat-fixation: approaches to the effective collection, fixation and preservation of trematodes of fishes. Syst Parasitol 76:1–7PubMedGoogle Scholar
  111. 111.
    Aiken HM, Hayward CJ, Crosbie P et al (2008) Serological evidence of an antibody response in farmed southern bluefin tuna naturally infected with the blood fluke Cardicola forsteri. Fish Shellfish Immunol 25:66–75PubMedGoogle Scholar
  112. 112.
    Kirchhoff NT, Leef MJ, Valdenegro V et al (2012) Correlation of humoral immune response in southern bluefin tuna, T. maccoyii, with infection stage of the blood fluke, Cardicola forsteri. PLoS One 7:e45742PubMedCentralPubMedGoogle Scholar
  113. 113.
    Ogawa K, Ishimaru K, Shirakashi S et al (2011) Cardicola opisthorchis n. sp. (Trematoda: Aporocotylidae) from the Pacific bluefin tuna, Thunnus orientalis (Temminck & Schlegel, 1844), cultured in Japan. Parasitol Int 60:307–312PubMedGoogle Scholar
  114. 114.
    Repulles-Albelda A, Montero FE, Holzer AS et al (2008) Speciation of the Paradeontacylix spp. (Sanguinicolidae) of Seriola dumerili. Two new species of the genus Paradeontacylix from the Mediterranean. Parasitol Int 57:405–414PubMedGoogle Scholar
  115. 115.
    Polinski M, Hamilton DB, Nowak B et al (2013) SYBR, TaqMan, or both: Highly sensitive, non-invasive detection of Cardicola blood fluke species in Southern Bluefin Tuna (Thunnus maccoyii). Mol Biochem Parasitol 191:7–15Google Scholar
  116. 116.
    Kirchhoff NT, Rough KM, Nowak BF (2011) Moving cages further offshore: effects on southern bluefin tuna, T. maccoyii, parasites, health, and performance. PLoS One 6:e23705PubMedCentralPubMedGoogle Scholar
  117. 117.
    Shirakashi S, Andrews M, Kishimoto Y et al (2012) Oral treatment of praziquantel as an effective control measure against blood fluke infection in Pacific bluefin tuna (Thunnus orientalis). Aquaculture 326:15–19Google Scholar
  118. 118.
    Hardy-Smith P, Ellis D, Humphrey J et al (2012) In vitro and in vivo efficacy of anthelmintic compounds against blood fluke (Cardicola forsteri). Aquaculture 334:39–44Google Scholar
  119. 119.
    Hayward CJ, Ellis D, Foote D et al (2010) Concurrent epizootic hyperinfections of sea lice (predominantly Caligus chiastos) and blood flukes (Cardicola foresteri) in ranched southern bluefin tuna. Vet Parasitol 173:107–115PubMedGoogle Scholar
  120. 120.
    Dennis MM, Landos M, D’Antignana T (2011) Case-control study of epidemic mortality and Cardicola forsteri-associated disease in farmed southern bluefin tuna (Thunnus maccoyii) of South Australia. Vet Pathol 48:846–855PubMedGoogle Scholar
  121. 121.
    Shirakashi S, Kishimoto Y, Kinami R et al (2012) Morphology and distribution of blood fluke eggs and associated pathology in the gills of cultured Pacific bluefin tuna, Thunnus orientalis. Parasitol Int 61:242–249PubMedGoogle Scholar
  122. 122.
    Nolan MJ, Cribb TH (2005) Sanguinicola maritimus n. sp. (Digenea: Sanguinicolidae) from Labridae (Teleostei: Perciformes) of southern Australian waters. Syst Parasitol 61:99–106PubMedGoogle Scholar
  123. 123.
    Huňová K, Kašný M, Hampl V et al (2012) Radix spp.: identification of trematode intermediate hosts in the Czech Republic. Acta Parasitol 57:273–284PubMedGoogle Scholar
  124. 124.
    Kirk RS, Lewis JW (1994) Sanguinicoliasis in cyprinid fish in the UK. In: Pike AW, Lewis JW (eds) Parasitic diseases of fish. Samara Publishing, WalesGoogle Scholar
  125. 125.
    Flint M, Blair D, Patterson-Kane J et al (2010) Blood flukes (Spirorchiidae) as a major cause of marine turtle mortality in Queensland. In: Proceedings of the XII international congress of parasitology (ICOPA), Melbourne, Australia, 15–20 Aug 2010, Medimond, Italy, pp. 57–61Google Scholar
  126. 126.
    Werneck MR, Gallo BMG, Silva RJ (2008) Spirorchiids (Digenea: Spirorchiidae) infecting a hawksbill sea turtle Eretmochelys imbricata (Linnaeus 1758) from Brazil. Arq Brasil Med Vet Zootech 60:663–666Google Scholar
  127. 127.
    Snyder SD (2004) Phylogeny and paraphyly among tetrapod blood flukes (Digenea: Schistosomatidae and Spirorchiidae). Int J Parasitol 34:1385–1392PubMedGoogle Scholar
  128. 128.
    Platt TR, Brooks DR (1997) Evolution of the schistosomes (Digenea: Schistosomatoidea): the origin of dioecy and colonization of the venous system. J Parasitol 83:1035–1044PubMedGoogle Scholar
  129. 129.
    Platt TR (1992) A phylogenic and biogeographic analysis of the genera of Spirorchinae (Digenea: Spirorchidae) parasitic in freshwater turtles. J Parasitol 78:616–629PubMedGoogle Scholar
  130. 130.
    Platt TR (2002) Family Spirorchiidae Stunkard, 1921. In: Gibson DI, Jones A, Bray RA (eds) Keys to the Trematoda, vol 1. CAB International/The Natural History Museum, LondonGoogle Scholar
  131. 131.
    Holliman RB, Fisher JE (1968) Life cycle and pathology of Spirorchis scripta Stunkard, 1923 (Digenea: Spirorchiidae) in Chrysemys picta picta. J Parasitol 54:310–318PubMedGoogle Scholar
  132. 132.
    Holliman RB, Fisher JE, Parker JC (1971) Studies on Spirorchis parvus (Stunkard, 1923) and its pathological effects on Chrysemys picta picta. J Parasitol 57:71–77Google Scholar
  133. 133.
    Glazebrook JS, Campbell RS, Blair D (1981) Pathological changes associated with cardiovascular trematodes (Digenea: Spirorchidae) in a green sea turtle Chelonia mydas (L). J Comp Pathol 91:361–368PubMedGoogle Scholar
  134. 134.
    Santoro M, Morales JA, Rodríguez-Ortíz B (2007) Spirorchiidiosis (Digenea: Spirorchiidae) and lesions associated with parasites in Caribbean green turtles (Chelonia mydas). Vet Rec 161:482–486PubMedGoogle Scholar
  135. 135.
    Stacy BA, Foley AM, Greiner E et al (2010) Spirorchiidiasis in stranded loggerhead Caretta caretta and green turtles Chelonia mydas in Florida (USA): host pathology and significance. Dis Aquat Org 89:237–259PubMedGoogle Scholar
  136. 136.
    Chen HC, Kuo RJ, Chang TC et al (2012) Fluke (Spirorchiidae) infections in sea turtles stranded on Taiwan: prevalence and pathology. J Parasitol 98:437–439PubMedGoogle Scholar
  137. 137.
    Aguirre AA, Spraker TR, Balazs GH et al (1998) Spirorchidiasis and fibropapillomatosis in green turtles from the Hawaiian islands. J Wildl Dis 34:91–98PubMedGoogle Scholar
  138. 138.
    Herbst LH, Jacobson ER, Klein PA et al (1999) Comparative pathology and pathogenesis of spontaneous and experimentally induced fibropapillomas of green turtles (Chelonia mydas). Vet Pathol 36:551–564PubMedGoogle Scholar
  139. 139.
    Snyder SD, Clopton RE (2005) New methods for the collection and preservation of spirorchiid trematodes and polystomatid monogeneans from turtles. Comp Parasitol 72:10–107Google Scholar
  140. 140.
    Adnyana W, Ladds PW, Blair D (1997) Efficacy of praziquantel in the treatment of green sea turtles with spontaneous infection of cardiovascular flukes. Aust Vet J 75:405–407PubMedGoogle Scholar
  141. 141.
    Jacobson ER, Harman GR, Maxwell LK et al (2003) Plasma concentrations of praziquantel in loggerhead sea turtles, Caretta caretta, following oral administration of single and multiple doses. Am J Vet Res 64:304–308PubMedGoogle Scholar
  142. 142.
    Platt TR, Blair D, Purdie J et al (1991) Griphobilhazia amoena n. sp. (Digenea: Schistosomatidae), a parasite of the freshwater crocodile Crocodylus johnstoni (Reptilia: Crocodylia) from Australia, with the erection of a new subfamily, Griphobilharziinae. J Parasitol 77:65–68Google Scholar
  143. 143.
    Khalil LF (2002) Family Schistosomatidae Stiles & Hassall, 1898. In: Gibson DI, Jones A, Bray RA (eds) Keys to the Trematoda, vol 1. CAB International/The Natural History Museum, LondonGoogle Scholar
  144. 144.
    Brant SV, Loker ES (2005) Can specialized pathogens colonize distantly related hosts? Schistosome evolution as a case study. PLoS Pathog 1:e38PubMedCentralGoogle Scholar
  145. 145.
    Platt TR, Hoberg EP, Chisholm LA (2013) On the morphology and taxonomy of Griphobilharzia amoena Platt and Blair, 1991 (Schistosomatoidea), a dioecious digenetic trematode parasite of the freshwater crocodile, Crocodylus johnstoni, in Australia. J Parasitol 99:888–891Google Scholar
  146. 146.
    Möhl K, Große K, Hamedy A (2009) Biology of Alaria spp. and human exposition risk to Alaria mesocercariae—a review. Parasitol Res 105:1–15PubMedGoogle Scholar
  147. 147.
    Foster GW, Kinsella JM, Sheppard BJ et al (2009) Transmammary infection of free-ranging Florida panther neonates by Alaria marcianae (Trematoda: Diplostomatidae). J Parasitol 95:238–239PubMedGoogle Scholar
  148. 148.
    Moks E, Jõgisalu I, Saarma U et al (2006) Helminthologic survey of the wolf (Canis lupus) in Estonia, with an emphasis on Echinococcus granulosus. J Wildl Dis 42:359–365PubMedGoogle Scholar
  149. 149.
    Kozlowska J (1957) On the knowledge of the helminth fauna of wild and bred foxes. Acta Parasitol Pol 5:181–192Google Scholar
  150. 150.
    Schuster R, Schierhorn K, Heidecke D et al (1993) Untersuchungen zur Endoparasitenfauna des Marderhundes Nyctereutes procynoides (Gray, 1834) in Ostdeutschland. Beitr Jagd Wildforschung 18:83–87Google Scholar
  151. 151.
    Freeman RS, Stuart PF, Cullen SJ et al (1976) Fatal human infection with mesocercariae of the trematode Alaria americana. Am J Trop Med Hyg 25:803–807PubMedGoogle Scholar
  152. 152.
    Riehn K, Hamedy A, Große K et al (2010) A novel detection method for Alaria alata mesocercariae in meat. Parasitol Res 107:213–220PubMedGoogle Scholar
  153. 153.
    Riehn K, Hamedy A, Alter T et al (2011) Development of a PCR approach for differentiation of Alaria spp. mesocercariae. Parasitol Res 108:1327–1332PubMedGoogle Scholar
  154. 154.
    Niewiadomska K (1996) The genus Diplostomum—taxonomy, morphology and biology. Acta Parasitol 41:55–66Google Scholar
  155. 155.
    Niewiadomska K (2002) Family Diplostomidae Poirier, 1886. In: Gibson DI, Jones A, Bray RA (eds) Keys to the Trematoda, vol 1. CAB International/The Natural History Museum, LondonGoogle Scholar
  156. 156.
    Chappell LH (1995) The biology of diplostomatid eyeflukes of fishes. J Helminthol 69:97–101PubMedGoogle Scholar
  157. 157.
    Georgieva S, Soldánová M, Perez-del-Olmo A et al (2013) Molecular prospecting for European Diplostomum (Digenea: Diplostomidae) reveals cryptic diversity. Int J Parasitol 43:57–72PubMedGoogle Scholar
  158. 158.
    Locke SA, Mclaughlin JD, Dayanandan S et al (2010) Diversity and specificity in Diplostomum spp. metacercariae in freshwater fishes revealed by cytochrome c oxidase I and internal transcribed spacer sequences. Int J Parasitol 40:333–343PubMedGoogle Scholar
  159. 159.
    Galazzo DE, Dayanandan S, Marcogliese DJ et al (2002) Molecular systematics of some North American species of Diplostomum (Digenea) based on rDNA-sequence data and comparisons with European congeners. Can J Zool 80:2207–2217Google Scholar
  160. 160.
    Lester RJG, Lee TDG (1976) Infectivity of the progenetic metacercaria of Diplostomum spathaceum. J Parasitol 62:832–833PubMedGoogle Scholar
  161. 161.
    Riley EM, Chappell LH (1992) Effect of infection with Diplostomum spathaceum on the internal defense system of Lymnaea stagnalis. J Invertebr Pathol 59:190–196Google Scholar
  162. 162.
    Karvonen A, Kirsi S, Hudson PJ et al (2004) Patterns of cercarial production from Diplostomum spathaceum: terminal investment or bet hedging? Parasitology 129:87–92PubMedGoogle Scholar
  163. 163.
    Niewiadomska K (1984) Present status of Diplostomum spathaceum (Rudolphi, 1819) and differentiation of Diplostomum pseudospathaceum nom. nov. (Trematoda: Diplostomatidae). Syst Parasitol 6:81–86Google Scholar
  164. 164.
    Niewiadomska K, Kiseliene V (1994) Diplostomum cercariae (Digenea) in snails from Lithuania. II. Survey of species. Acta Parasitol 39:179–186Google Scholar
  165. 165.
    Haas W, Stiegeler P, Keating A et al (2002) Diplostomum spathaceum cercariae respond to a unique profile of cues during recognition of their fish host. Int J Parasitol 32:1145–1154PubMedGoogle Scholar
  166. 166.
    Moczoň T (1994) Histochemistry of proteinases in the cercariae of Diplostomum pseudospathaceum (Trematoda, Diplostomatidae). Parasitol Res 80:684–686PubMedGoogle Scholar
  167. 167.
    Mikeš L, Man P (2003) Purification and characterization of a saccharide-binding protein from penetration glands of Diplostomum pseudospathaceum—a bifunctional molecule with cysteine protease activity. Parasitology 127:69–77PubMedGoogle Scholar
  168. 168.
    Ratanarat-Brockelman C (1974) Migration of Diplostomum spathaceum (Trematoda) in the fish intermediate host. Z Parasitenkd 43:123–134Google Scholar
  169. 169.
    Haas W, Wulff C, Grabe K et al (2007) Navigation within host tissues: cues for orientation of Diplostomum spathaceum (Trematoda) in fish towards veins, head and eye. Parasitology 134:1013–1023PubMedGoogle Scholar
  170. 170.
    Whyte SK, Secombes CJ, Chappell LH (1991) Studies on the infectivity of Diplostomum spathaceum in rainbow trout (Oncorhynchus mykiss). J Helminthol 65:169–178PubMedGoogle Scholar
  171. 171.
    Chappell LH, Hardie LJ, Secombes CJ (1994) Diplostomiasis: the disease and host-parasite interactions. In: Pike AW, Lewis JW (eds) Parasitic diseases of fish. Samara Publishing, WalesGoogle Scholar
  172. 172.
    Karvonen A (2012) Diplostomum spathaceum and related species. In: Woo PTK, Buchmann K (eds) Fish parasites, pathobiology and protection. CAB International, LondonGoogle Scholar
  173. 173.
    Barber I, Crompton DWT (1997) The distribution of the metacercariae of Diplostomum phoxini in the brain of minnows, Phoxinus phoxinus. Folia Parasitol 44:19–25PubMedGoogle Scholar
  174. 174.
    Dezfuli BS, Capuano S, Simoni E et al (2007) Histopathological and ultrastructural observations of metacercarial infections of Diplostomum phoxini (Digenea) in the brain of minnows Phoxinus phoxinus. Dis Aquat Organ 75:51–59PubMedGoogle Scholar
  175. 175.
    Heckmann RA (1992) Host records and tissue locations for Diplostomum mordax (metacercariae) inhabiting the cranial cavity of fishes from Lake Titicaca, Peru. J Parasitol 78:541–543PubMedGoogle Scholar
  176. 176.
    Siegmund I, Franjola R, Torres P (1997) Diplostomatid metacercariae in the brain of silversides from Lake Rinihue, Chile. J Wildl Dis 33:362–364PubMedGoogle Scholar
  177. 177.
    Hendrickson GL (1979) Ornithodiplostomum ptychocheilus: migration to the brain of the fish intermediate host, Pimephales promelas. Exp Parasitol 48:245–258PubMedGoogle Scholar
  178. 178.
    Matisz CE, Goater CP, Bray D (2010) Migration and site selection of Ornithodiplostomum ptychocheilus (Trematoda: Digenea) metacercariae in the brain of fathead minnows (Pimephales promelas). Parasitology 137:719–731PubMedGoogle Scholar
  179. 179.
    Lester RJG, Freeman RS (1976) Survival of 2 trematode parasites (Diplostomum spp.) in mammalian eyes and associated pathology. Can J Ophthalmol 11:229–234PubMedGoogle Scholar
  180. 180.
    Karvonen A, Savolainen M, Seppälä O et al (2006) Dynamics of Diplostomum spathaceum infection in snail hosts at a fish farm. Parasitol Res 99:341–345PubMedGoogle Scholar
  181. 181.
    Buchmann K, Bresciani J (1997) Parasitic infections in pond-reared rainbow trout Oncorhynchus mykiss in Denmark. Dis Aquat Organ 28:125–138Google Scholar
  182. 182.
    Soldánová M, Kuris AM, Scholz T et al (2012) The role of spatial and temporal heterogeneity and competition in structuring trematode communities in the great pond snail, Lymnaea stagnalis (L.). J Parasitol 98:460–471PubMedGoogle Scholar
  183. 183.
    Karvonen A, Seppälä O, Valtonen ET (2004) Eye fluke-induced cataract formation in fish: quantitative analysis using an ophthalmological microscope. Parasitology 129:473–478PubMedGoogle Scholar
  184. 184.
    Rellstab C, Louhi KR, Karvonen A et al (2011) Analysis of trematode parasite communities in fish eye lenses by pyrosequencing of naturally pooled DNA. Infect Genet Evol 11:1276–1286PubMedGoogle Scholar
  185. 185.
    Field JS, Irwin SWB (1994) The epidemiology, treatment and control of diplostomiasis on a fish farm in Northern Ireland. In: Pike AW, Lewis JW (eds) Parasitic diseases of fish. Samara, WalesGoogle Scholar
  186. 186.
    Speed P, Pauley GB (1985) Feasibility of protecting rainbow trout, Salmo gairdneri Richardson, by immunizing against the eye fluke, Diplostomum spathaceum. J Fish Biol 26:739–744Google Scholar
  187. 187.
    Whyte SK, Chappell LH, Secombes CJ (1990) Protection of rainbow trout, Oncorhynchus mykiss (Richardson), against Diplostomum spathaceum (Digenea): the role of specific antibody and activated macrophages. J Fish Dis 13:281–291Google Scholar
  188. 188.
    Karvonen A, Halonen H, Seppälä O (2010) Priming of host resistance to protect cultured rainbow trout Oncorhynchus mykiss against eye flukes and parasite-induced cataracts. J Fish Biol 76:1508–1515PubMedGoogle Scholar
  189. 189.
    Lane RL, Morris JE (2000) Biology, prevention, and effects of common grubs (digenetic trematodes) in freshwater fish. Tech Bull Ser Iowa State Univ 115:1–6Google Scholar
  190. 190.
    Wittrock DD, Bruce CS, Johnson AD (1991) Histochemistry and ultrastructure of the metacercarial cysts of blackspot trematodes Uvulifer ambloplitis and Neascus pyriformis. J Parasitol 77:454–460PubMedGoogle Scholar
  191. 191.
    Tobler M, Schlupp I (2008) Influence of black spot disease on shoaling behaviour in female western mosquitofish, Gambusia affinis (Poeciliidae, Teleostei). Environ Biol Fish 81:29–34Google Scholar
  192. 192.
    Ondračková M, Dávidová M, Gelnar M et al (2006) Susceptibility of Prussian carp infected by metacercariae of Posthodiplostomum cuticola (v. Nordmann, 1832) to fish predation. Ecol Res 21:526–529Google Scholar
  193. 193.
    Ondračková M, Šimková A, Gelnar M et al (2004) Posthodiplostomum cuticola (Digenea: Diplostomatidae) in intermediate fish hosts: factors contributing to the parasite infection and prey selection by the definitive bird host. Parasitology 129:761–770PubMedGoogle Scholar
  194. 194.
    Zrnčić S, Oraić D, Mihaljević Ž et al (2009) First observation of Posthodiplostomum cuticola (Nordmann, 1832) metacercariae in cypriniformes from Croatia. Helminthologia 46:112–116Google Scholar
  195. 195.
    Schleppe JL, Goater CP (2004) Comparative life-histories of two diplostomid trematodes, Ornithodiplostomum ptychocheilus and Posthodiplostomum minimum. J Parasitol 90:1387–1390PubMedGoogle Scholar
  196. 196.
    Sandland GJ, Goater CP, Danylchuk AJ (2001) Population dynamics of Ornithodiplostomum ptychocheilus metacercariae in fathead minnows (Pimephales promelas) from four northern-Alberta Lakes. J Parasitol 87:744–748PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Petr Horák
    • 1
  • Libuše Kolářová
    • 2
  • Libor Mikeš
    • 1
  1. 1.Department of Parasitology, Faculty of ScienceCharles University in PraguePragueCzech Republic
  2. 2.Institute of Immunology and Microbiology, First Faculty of MedicineCharles University in Prague and General University Hospital in PraguePragueCzech Republic

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