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Safflower (Carthamus tinctorius L.) Oil Content and Yield Components as Affected by Co-inoculation with Azotobacterchroococcum and Glomusintraradices at Various N and P Levels in a Dry Climate

  • Mohammad Mirzakhani
  • Mohammad Reza Ardakani
  • Farhad Rejali
  • Amir Hossein Shirani Rad
  • Mohammad MiransariEmail author
Chapter

Abstract

With respect to the significance of medicinal plants, testing methods, which may enhance their performance, can be important agriculturally and economically as well as from healthy points of view. Hence, grain oil content and yield components of the medicinal plant, safflower (Carthamus tinctorius L., cultivar IL-111), were evaluated in a field experiment. Treatments including co-inoculation with Azotobacter chroococcum and the Arbuscular mycorrhizal (AM) fungi Glomus intraradices at different rates of nitrogen (N) and phosphorous (P) fertilization including F0: control, F1: N50 + P25, F2 = N100 + P50, and F3 = N150 + P75 kg ha−1 were tested. The experiment was conducted in the spring of 2006 as a factorial on the basis of a completely randomized block design in three replicates. Grain oil content and yield components were determined. A. chroococcum increased plant N content. The two- (fungi and fertilization) and three-way (bacteria, fungi, and fertilization) interactions significantly affected grain oil content and weight of 1,000 grain, respectively. While chemical fertilization significantly decreased oil percentage, the bacterium and the fungus significantly increased oil percentage. However, for the oil content the combination of microbes with chemical fertilization resulted in the highest oil content. The effective co-inoculation of safflower with A. chroococcum and G. intraradices by increasing oil content and yield components suggests the effectiveness of this biological method for safflower production as well as a favorite partial replacement for N and P fertilization. The effects of biological fertilization on the enhancement of seed nutritional values may be more pronounced than chemical fertilization.

Keywords

Azotobacterchroococcum Glomusintraradices Oil content N and P fertilizer Safflower (Carthamus tinctorius L.) 

References

  1. Basalma D, Uranbey S, Mirici S, Kolsarici Ö (2008) TDZ x IBA induced shoot regeneration from cotyledonary leaves and in vitro multiplication in safflower (Carthamus tinctorius L.). Afric J Biotechnol 7:960–966Google Scholar
  2. Behl RK, Sharma H, Kumar V, Singh KP (2003) Effect of dual inoculation of mycorrhiza and Azotobacter on above flag leaf characters in wheat. Arch Agron Soil Sci 49:25–31CrossRefGoogle Scholar
  3. Bryla DR, Duniway JM (1997) Water uptake by safflower and wheat roots infected with arbuscular mycorrhiza fungi. New Phytol 136:591CrossRefGoogle Scholar
  4. Diaz FA, Garza I, Ortegon AS (2006) Biofertilization of Safflower (Carthamus tintorius) under limited humidity conditions. Rev Fitotec Mex 29:175–180Google Scholar
  5. Elfadl E, Reinbrecht C, Claupein W (2010) Evaluation of phenotypic variation in a worldwide germplasm collection of safflower (Carthamus tinctorius L.) grown under organic farming conditions in Germany. Genet Resour Crop Evol 57:155–170CrossRefGoogle Scholar
  6. Foruzaun K (1999) Safflower. Oil Grain Company, Persian, p 151Google Scholar
  7. Gecgel U, Demirci M, Esendal E, Tasan M (2007) Fatty acid composition of the oil from developing seeds of different varieties of safflower (Carthamus tinctorius L.). J Am Oil Chem Soc 84:47–54CrossRefGoogle Scholar
  8. Gunasekera CP, Martin LD, Siddique KHM, Walton GH (2006) Genotype by environment interactions of Indian mustard (Brassica juncea L.) and canola (Brassica napus L.) in Mediterranean-type environments II. Oil and protein concentrations in seed. Eur J Agron 25:3–21CrossRefGoogle Scholar
  9. Khajeh Pour MR (1991) Production of industrial crops. Isfahan University of Technology, Isfahan, Iran, Persian, p 251Google Scholar
  10. Lal L (2000) Phosphatic biofertilizer. Agrotech Publishing Academy, India, p 224Google Scholar
  11. Marschner H (1995) Mineral nutrition of higher plants. Academic Press, London, UKGoogle Scholar
  12. Li H, Dong Y, Sun Y, Zhu E, Yang J, Liu X, Xue P, Xiao Y, Yang S, Wu J, Li X (2010) Investigation of the microRNAs in safflower seed, leaf, and petal by high-throughput sequencing. Planta 233:611–619Google Scholar
  13. Miransari M, Bahrami HA, Rejali F, Malakouti MJ, Torabi H (2007) Using arbuscular mycorrhiza to reduce the stressful effects of soil compaction on corn (Zea mays L.) growth. Soil Biol Biochem 39:2014–2026CrossRefGoogle Scholar
  14. Miransari M, Bahrami HA, Rejali F, Malakouti MJ (2008) Using arbuscular mycorrhiza to reduce the stressful effects of soil compaction on wheat (Triticum aestivum L.) growth. Soil Biol Biochem 40:1197–1206CrossRefGoogle Scholar
  15. Miransari M, Rejali F, Bahrami HA, Malakouti MJ (2009a) Effects of soil compaction and arbuscular mycorrhiza on corn (Zea mays L.) nutrient uptake. Soil Till Res 103:282–290CrossRefGoogle Scholar
  16. Miransari M, Rejali F, Bahrami HA, Malakouti MJ (2009b) Effects of arbuscular mycorrhiza, soil sterilization, and soil compaction on wheat (Triticum aestivum L.) nutrients uptake. Soil Till Res 104:48–55Google Scholar
  17. Miransari M (2010a) Contribution of arbuscular mycorrhizal symbiosis to plant growth under different types of soil stresses. Plant Biol 12:563–569PubMedGoogle Scholar
  18. Miransari M (2010b) Biological Fertilization. In: Méndez-Vilas A (ed.) Current research, technology and education topics in applied microbiology and microbial biotechnology. Microbiology Book Series—2010 Edition, SpainGoogle Scholar
  19. Miransari M (2011a) Interactions between arbuscular mycorrhizal fungi and soil bacteria. Appl Microbiol Biotechnol 89:917–930Google Scholar
  20. Miransari M (2011b) Arbuscular mycorrhizal fungi and nitrogen uptake. Arch Microbiol 193:77–81Google Scholar
  21. Mirzakhani M, Ardakani MR, Aeene Band A, Shirani Rad AH, Rejali F (2009) Effects of dual inoculation of azotobacter and mycorrhiza with nitrogen and phosphorus fertilizer rates on grain yield and some of characteristics of spring safflower. Internat J Environ Sci Engin 1:39–43Google Scholar
  22. Miransari M (2013a) Soil microbes and the availability of soil nutrients. Acta Physiol Plant 35:3075–3084CrossRefGoogle Scholar
  23. Miransari M (2013b) Plant growth promoting rhizobacteria. J Plant Nutr (in press)Google Scholar
  24. Miransari M. et al (2013a). Plant hormones as signals in arbuscular mycorrhizal symbiosis. Crit Rev Biotechnol (in press)Google Scholar
  25. Miransari M et al (2013b) Improving soybean (Glycine max L.) N2-fixation under stress. J Plant Growth Regul 32:909–921Google Scholar
  26. Omidi H, Tahmasebi Z, Naghdi-Badi HA, Torabi H, Miransari M (2010) Fatty acid composition of canola (Brassica napus. L), as affected by agronomical, genotypic and environmental parameters. Comp Ren Biol 333:248–254CrossRefGoogle Scholar
  27. Rahamatalla AB, Babiker EE, Krishna AG, El Tinay AH (2001) Changes in fatty acids composition during seed growth and physiocochemical characterstics of oil extracted from four safflower cultivars. Plant Food Human Nutr 56:385–395CrossRefGoogle Scholar
  28. Samanci B, Ozkaynak E (2003) Effect of planting date on seed yield, oil content and fatty acid composition of the safflower cultivars grown in the mediterranean region of Turkey. J Agron Crop Sci 189:359–360CrossRefGoogle Scholar
  29. Seddiqui ZA, Mahmood I (2001) Effect of rhizobacteria and root symbionts on the reproduction of Meloidogyne javanica and growth of chickpea. Bioresource Technol. 79:41–45CrossRefGoogle Scholar
  30. Sharma AK, Johri BN (eds) (2002) Arbuscular mycorrhizae, interaction in plants, rhizosphere and soils. Oxford and IBH Publishing, New Delhi, p 308Google Scholar
  31. Shenoy VV, Kalagudi GM (2005) Enhancing plant phosphorus use efficiency for sustainable cropping. Biotechnol Adv 23:501–513PubMedCrossRefGoogle Scholar
  32. Smith SE, Read DJ (1997) Mycorrhizal symbiosis. Academic Press, London, pp 126–160CrossRefGoogle Scholar
  33. Steel RGD, Torrie JH (1980) Principles and procedures of statistics: a biometrical approach. 2nd (edn) McGraw-Hill Book CompanyGoogle Scholar
  34. Thippeswamy M, Chandraobulreddy P, Sinilal B, Kumar M (2010) Proline accumulation and the expression of Δ1-pyrroline-5-carboxylate synthetase in two safflower cultivars. Biol Plant 54:386–390CrossRefGoogle Scholar
  35. Velasco L, Fernandez-Martinez J (2001) Breeding for oil quality in safflower. In: Bergman JW, Mundel HH (eds) Proceedings of the 5th international safflower conference. Williston, North Dakota and Sidney, Montana, USA, pp 133–137Google Scholar
  36. Wu ZH, Cao ZG, Li KC, Cheung MH, Wong SC (2005) Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma 125:155–166CrossRefGoogle Scholar
  37. Zabihi HR, Savaghebi GR, Khavazi K, Ganjali A, Miransari M (2011) Pseudomonas bacteria and phosphorous fertilization, affecting wheat (Triticum aestivum L.) yield and P uptake under greenhouse and field conditions. Acta Physiol Plant 33:145–152Google Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Mohammad Mirzakhani
    • 2
  • Mohammad Reza Ardakani
    • 3
  • Farhad Rejali
    • 4
  • Amir Hossein Shirani Rad
    • 5
  • Mohammad Miransari
    • 1
    Email author
  1. 1.Department of Book and ArticleAbtinBerkeh Limited Co.IsfahanIran
  2. 2.Farahan Islamic Azad UniversityArakIran
  3. 3.Department of Agronomy and Plant BreedingKaraj Islamic Azad UniversityKarajIran
  4. 4.Division of Soil BiologySoil and Water Research Institute KarajIran
  5. 5.Seed and Plant Improvement InstituteKarajIran

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