DNA Metabolism and Cell Proliferation

  • Joseph Altman
Chapter

Abstract

In considering the problem of DNA metabolism and cell proliferation in the central nervous system we shall review in this chapter results obtained with three major techniques: (1) histological and cytological studies of cell division, which in some investigations were coupled with the auxiliary procedure of blocking mitosis with a mitotic poison, such as colchicine; (2) gross and microchemical studies of differences in DNA concentration in the brain in different species, at different phases of development or in different regions of the brain; and (3) studies in which a radioactively labeled specific precursor of DNA was administered to animals to tag newly forming cells and these were rendered visible and their fate traced in situ with auto-radiography. Because in the history of this topic the histological approach antedated the other two, we shall introduce the subject by describing briefly the results of early histological studies. These early studies generated concepts that had a lasting influence on investigations in this area, but these have been supplemented, modified and, to some extent, superseded by results obtained with more modern techniques.

Keywords

Purkinje Cell Granule Cell Olfactory Bulb Cerebellar Cortex Granular Layer 
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Copyright information

© Springer Science+Business Media New York 1969

Authors and Affiliations

  • Joseph Altman
    • 1
  1. 1.Laboratory of Developmental Neurobiology Department of Biological SciencesPurdue UniversityLafayetteUSA

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