Advertisement

Cloning, Expression and Biological Activity of the Juvenile Hormone Esterase from Heliothis virescens

  • B. Hammock
  • V. Wroblewski
  • L. Harshman
  • T. Hanzlik
  • S. Maeda
  • M. Philpott
  • B. Bonning
  • R. Possee

Abstract

One of the more exciting trends in science is the increasingly short space of time between many fundamental discoveries and their impact on applied science. In turn, applied problems increasingly can be addressed directly by fundamental approaches. While solving such applied problems one often sees an advancement of the fundamental field as well. These trends certainly are illustrated by our recent work on the juvenile hormone esterases (JHE’s) of lepidopterous larvae. Expression of this enzyme in several baculovirus systems may have impact on pest control and viral ecology, but certainly this work will advance our fundamental knowledge of insect endocrinology.

Keywords

Juvenile Hormone Recombinant Virus Catalytic Triad Polyadenylation Site Heliothis Virescens 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abdel-Aal, Y.A.I. and Hammock, B.D. 1986. Transition state analogs as ligands for affinity purification of juvenile hormone esterase. Science 233: 1073–1076.PubMedCrossRefGoogle Scholar
  2. Blow, D.M. 1976. Structure and mechanism of chymotrypsin. Accounts Chem. Res. 9: 145–152.CrossRefGoogle Scholar
  3. Hammock, B.D. 1985. Regulation of juvenile hormone titer: degradation. pp. 431–472 in: “Comprehensive Insect Physiology Biochemistry and Pharmacology.” Kerkut, G.A. and Gilbert, L.I. eds. Pergamon Press, N.Y.Google Scholar
  4. Hammock, B.D. and Sparks, T.C. 1977. A rapid assay for insect juvenile hormone esterase activity. Anal. Biochem. 82: 573–579.PubMedCrossRefGoogle Scholar
  5. Hammock, B.D., Abdel-Aal, Y.A.I., Mullin, CA., Hanzlik, T.N. and Roe, R.M. 1984. Substituted thiotrifluoropropanones as potent selective inhibitors of juvenile hormone esterase. Pestic. Biochem. Physiol. 22: 209–223.CrossRefGoogle Scholar
  6. Hammock, B.D., Harshman, L.G., Philpott, M.L., Szekacs, A., Ottea, J.A., Newitt, R.A., Wroblewski, V.J., Halarnkar, P.P. and Hanzlik, T.N. 1988. Strategies for the discovery of insect control agents: Exploitation of biomechanisms regulating insect development. pp. 137–173 in: “Biomechanisms Regulating Growth and Development.” USDA Beltsville Symposia in Agricultural Research, Vol.12. Steffens, G.L. and Rumsey, T.S. eds. Kluwer Academic Publishers, Dordrecht, The Netherlands.Google Scholar
  7. Hammock, B. D., Bonning, B., Possee, R. D., Hanzlik, T. N. and Maeda, S. 1990. Expression and effects of the juvenile hormone esterase in a baculovirus vector. Nature in press.Google Scholar
  8. Hanzlik, T.N. and Hammock, B.D. 1988. Characterization of juvenile hormone hydrolysis in early larval development of Trichoplusia ni. Arch. Insect Biochem. Physiol. 9: 135–156.CrossRefGoogle Scholar
  9. Hanzlik, T.N., Abdel-Aal, Y.A.I., Harshman, L.G. and Hammock, B.D. 1989. Isolation and sequencing of cDNA clones coding for juvenile hormone esterase from Heliothis virescens. J. Biol. Chem. 264: 12419–12425.PubMedGoogle Scholar
  10. Jones, G., Hanzlik, T., Hammock, B.D., Schooley, D.A., Miller, CA., Tsai, L.W. and Baker, F.C. 1989. The juvenile hormone titre during the penultimate and ultimate larval stadia of Trichoplusia ni. J. Ins. Physiol. in press.Google Scholar
  11. Maeda, S. 1989. Gene transfer vectors of a baculovirus, Bombyx mori nuclear polyhedrosis virus, and their use for expression of foreign genes in insect cells. pp. 167–181 in: “Invertebrate cell system applications.” Vol. I. Mitsuhashi, J. ed. CRC Press, Boca Raton, Fl..Google Scholar
  12. Matsuura, Y., Possee, R.D., Overton, H.A. and Bishop, H.L. 1987. Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. J. Gen. Virol. 68: 1233–1250.PubMedCrossRefGoogle Scholar
  13. Philpott, M.L. and Hammock, B.D. 1989. Juvenile hormone esterase is a biochemical anti- juvenile hormone agent. Insect Biochem. in press.Google Scholar
  14. Smith, S.O., Farr-Jones, S., Griffin, R.G., and Bachovchin, W.W. 1989. Crystal vs. solution structures of enzymes NMR spectroscopy of a crystalline serine protease. Science 244: 961–964.PubMedCrossRefGoogle Scholar
  15. Sparks, T.C. and Hammock, B.D. 1980. Comparative inhibition of the juvenile hormone esterases from Trichoplusia ni, Tenebrio monitor, and Musca domestica. Pestic. Biochem. Physiol. 14: 290–302.CrossRefGoogle Scholar
  16. Weirich, G., Wren, J. and Siddall, J.B. 1973. Developmental changes of the juvenile hormone esterase activity in haemolymph of the tobacco hornworm, Manduca sexta. Insect Biochem. 3: 397–407.CrossRefGoogle Scholar
  17. Wroblewski, V.J., Harshman, L.G., Hanzlik, T.N. and Hammock, B.D. 1989. Regulation of juvenile hormone esterase gene expression in the tobacco budworm, (Heliothis virescens). Arch. Biochem. Biophys. in press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • B. Hammock
    • 1
  • V. Wroblewski
    • 1
  • L. Harshman
    • 1
  • T. Hanzlik
    • 1
  • S. Maeda
    • 2
  • M. Philpott
    • 1
  • B. Bonning
    • 3
  • R. Possee
    • 3
  1. 1.Departments of Entomology and Environmental ToxicologyUniversity of CaliforniaDavisUSA
  2. 2.Department of EntomologyUniversity of CaliforniaDavisUSA
  3. 3.NERC Institute of Virology and Environmental MicrobiologyOxfordUK

Personalised recommendations