Flow Cytometry versus Fluorescence Microscopy
Since the pioneer work of the botanist Matthias Jakob Schleiden and the zoologist Theodor Schwann in 1839, and of Rudolph Virchow in 1859, cell research progressed in two opposite directions. Cell biologists focused their increasingly more powerful microscopies into the cell structure to reveal the great morphological complexity of the cytoplasm. A growing number of subcellular organelles thus challenged the early biochemists to discover their specific molecular features and their coordination to maintain an ordered cell life. The biochemists’ answer to such a challenge consisted usually in tearing apart cells into their discrete components and obtaining information on molecules and pathways of each single part. The assembly of this jigsaw puzzle into an integrated view of a functional cell, and of such a cell within higher levels of organization, could not be achieved exclusively by pure biochemical methods. As early as in 1961, Jean Brachet wrote that “The cell biologist tries to explain in molecular terms what he sees under his microscope; he has become a molecular biologist. The biochemist in turn has become a biochemical cytologist, equally interested in the structure of the cell and the biochemical activity in which it is involved” (1).
KeywordsFlow Cytometry Fluorescent Marker Subcellular Organelle Jigsaw Puzzle Exogenous Marker
Unable to display preview. Download preview PDF.
- 1.Brachet J. The living cell. In: The Living Cell, W.H. Freeman & Co., San Francisco, p. 13, 1961.Google Scholar
- 2.Melamed MR, Mullaney PF and Shapiro HM. An historical review of the development of flow cytometry instruments. In: Flow Cytometry and Sorting, 2nd Ed. (Melamed, Lindmo, Mendelsohn, Eds.), Wiley-Liss, New York, 1990, pp. 1–10.Google Scholar
- 3.Shapiro HM. Practical Flow Cytometry, 3rd. Ed., Alan R. Liss, Inc., New York, 1995Google Scholar
- 4.Robinson JP. Links between flow and image cytometry. Proceedings of the IV Meeting of the Iberian Society of Cytometry, Oviedo (Spain), 20–24 May 1995, pp. 32-33.Google Scholar
- 5.Slavík J. Fluorescence Probes in Cellular and Molecular Biol. ogy, CRC Press, Inc., Boca Raton, 1994.Google Scholar
- 6.Bauer KD, Duque RE and Shankey TV, eds. Clinical Flow Cytometry: Principles and Applications Williams and Wilkins, Baltimore, 1993.Google Scholar
- 7.Lloyd D, ed. Flow Cytometry in Microbiology. Springer, London, 1993.Google Scholar