Variation in Virulence in the Gnotobiotic Pig Model of O157:H7 Escherichia Coli Strains of Bovine and Human Origin

  • Diane R. Baker
  • Rodney A. Moxley
  • David H. Francis
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 412)


Escherichia coli strains of serotype O157:H7 have been incriminated in outbreaks and sporadic cases of food-borne illness, including diarrhea, hemorrhagic colitis, hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. Food-producing animals, particularly cattle, are believed to be reservoirs of the organism. Whether all strains of bovine origin pose human health risk is unknown and was the impetus for this investigation. We compared the virulence of ten SLT-I, SLT-II, and eae DNA probe-positive O157:H7 strains from cattle to 10 like strains associated with human diarrheal disease outbreaks for virulence in one day-old gnotobiotic pigs. All strains caused diarrhea, and only four pigs inoculated with either of two bovine strains failed to develop that condition. Signs of central nervous system disease, death, debilitation requiring euthanasia before the end of an eight day observation period, and/or encephalomalacia occurred in 32/42 pigs inoculated with the strains isolated from human beings, 13/39 pigs inoculated with strains from cattle, and 7/7 pigs inoculated with a positive control strain. More strains of human origin (9/10) than bovine origin (5/10) caused these effects. The results of this study indicate considerable variability in virulence of O157:H7 strains possessing the same known virulence determinants, and suggest that disease outbreaks tend to be caused by the more virulent of these strains.


Hemolytic Uremic Syndrome Disease Outbreak Thrombotic Thrombocytopenic Purpura Central Nervous System Disease Human Origin 
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  1. Donnenberg, M. S., S. Tzipori, M. L. McKee, et al. 1993. The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model. J. Clin. Invest. 92: 1418–1424.Google Scholar
  2. Doyle, M. P., and J. L. Schoeni. 1987. Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry. App. Environ. Microbiol. 53: 2394–2396.Google Scholar
  3. Dykstra S. A., R. A. Moxley, B. H. Janke, et al. 1993. Clinical signs and lesions in gnotobiotic pigs inoculated with Shiga-like toxin I from Escherichia coli. Vet. Pathol. 30: 410–417.Google Scholar
  4. Francis, D. H., R. A. Moxley, and C. Y. Andraos. 1989. Edema disease-like brain lesions in gnotobiotic piglets infected with Escherichia coli serotype 0157:H7. Infect. Immun. 57: 1339–1342.Google Scholar
  5. Griffin, P. M. and R. V. Tauxe. 1991. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemmorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol. Rev. 13: 60–98.Google Scholar
  6. Hamano, S., Y. Nakanishi, T. Nara, et al. 1993. Neurological manifestations of hemorrhagic colitis in the outbreak of Escherichia coli infection in Japan. Acta Paediatr. 82: 454–458.PubMedCrossRefGoogle Scholar
  7. Hollingsworth, J. 1993. Role of federal agencies in controlling E. coli O157:H7. Pop. Med. News 6 (12): 10–11. Martin, M.L., L.D. Shipman, J.G. Wells, et al. 1986. Isolation of Escherichia coli O157:H7 from dairy cattle associated with two cases of haemolytic uraemic syndrome. Lancet 2: 1043.Google Scholar
  8. Ostroff, S. M., R. I. Tarr, M. A. Neill, et al. 1989. Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections. J. Infect. Dis. 160: 994–998.Google Scholar
  9. Ostroff, S. M., P. M. Griffin, R. V. Tauxe, et. al. 1990. A statewide outbreak of Escherichia coli O157:H7 infections in Washington State. AM. J. Epidemiol. 132: 239–247.Google Scholar
  10. Riley, L. W., R. S. Remis, S. D. Helgerson, et al. 1983. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308: 681–685.Google Scholar
  11. Riley, L. W. 1987. The epidemiologic, clinical, and microbiologic features of hemorrhagic colitis. Ann. Rev. Microbiol. 41: 383–407.Google Scholar
  12. Ryan, C. A., R. V. Tauxe, G. W. Hosek, et al. 1986. Escherichia coli O157:H7 diarrhea in a nursing home: clinical, epidemiological, and pathological findings. J. Infect. Dis. 154: 631–638.Google Scholar
  13. Tesh, V. L., and A. D. O’Brien. 1992. The pathogenetic mechanisms of Shiga toxin and the Shiga-like toxins. Mol. Microbiol. 5: 1817–1822.Google Scholar
  14. Tzipori, S., C. W. Chow, and H. R. Powell. 1988. Cerebral infection with Escherichia coli O157:H7 in humans and gnotobiotic piglets. J. Clin. Pathol. 41: 1099–1103.Google Scholar
  15. Wells, J.G., L.D. Shipman, K.D. Greene, et al. 1991. Isolation of Escherichia coli serotype O157:H7 and other Shiga-like toxin-producing E. coli from dairy cattle. J. Clin. Microbiol. 29: 985–989.Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Diane R. Baker
    • 1
  • Rodney A. Moxley
    • 2
  • David H. Francis
    • 1
  1. 1.Department of Veterinary ScienceSouth Dakota State UniversityBrookingsUSA
  2. 2.Department of Veterinary and Biomedical SciencesUniversity of Nebraska-LincolnLincolnUSA

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