Characterization of a Porcine Enterocyte Receptor for Group a Rotavirus

  • Mark S. Kuhlenschmidt
  • Mark D. Rolsma
  • Theresa B. Kuhlenschmidt
  • Howard B. Gelberg
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 412)

Summary

We have identified, purified to apparent homogeneity and chemically characterized a biologically-relevant porcine enterocyte receptor for group A porcine rotavirus. Ceramide glycanase digestion followed by acid hydrolysis and monosaccharide compositional analyses indicated the receptor is a family of two GM3 gangliosides, one containing N-glycolyl-neuraminic acid and the other N-acetylneuraminic acid. Both gangliosides displayed dose-dependent inhibition of rotavirus binding to, and infectivity of, host cells. Inhibition of infectivity in a focus-forming-unit-reduction assay was achieved with as little as 2 nmols of NeuGcGM3 (50% inhibition with 3.97 nmol) or NeuAcGM3 (50% inhibition with 9.84 nmol) per 104 FFU of virus. Preliminary data suggest specific porcine GM3 carbohydrate fine structure or spatial orientation of the sialyloligosaccharide epitopes of the holoGM3 gangliosides may be crucial to enterocyte receptor recognition by rotavirus. We have quantified both NeuGcGM3 and NeuAcGM3 in enterocytes of various-aged pigs from newborn through 16 weeks and have found with increasing age the amount of both GM3 derivatives, especially NeuGcGM3 per gram (dry weight) intestinal brush border decreases rapidly from newborn through 4 weeks of age. These results may help explain the age-sensitivity of piglets to severe rotavirus diarrhea.

Keywords

Sialic Acid Rota Virus Virus Binding Sialic Acid Moiety Ganglioside Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Mark S. Kuhlenschmidt
    • 1
  • Mark D. Rolsma
    • 2
  • Theresa B. Kuhlenschmidt
    • 1
  • Howard B. Gelberg
    • 1
  1. 1.Department of Veterinary Pathobiology College of Veterinary MedicineUniversity of IllinoisUrbanaUSA
  2. 2.Department of PathobiologyCollege of Veterinary MedicineAuburn UniversityUSA

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