Embryo Propagation by Direct Somatic Embryogenesis and Multiple Shoot Formation

Abstract

We are developing methods for rapid clonal propagation by direct somatic embryogenesis or direct multiple shoot formation in situ on immature zygotic emrbyos, with the following aims: a. to minimize culture-induced variation by avoiding cellular destabilization; b. to provide simple, rapid, space-efficient propagation techniques which can be applied as early in the life cycle as possible; c. to develop continuous embryogenic cultures with the potential for mechanized sterile propagation and harvesting.