In Vitro Regeneration of Plantlets from Cultured Tissues of Scots Pine (Pinus Sylvestris L.)

Abstract

After germination and 4 weeks of culture on a modified Murashige and Skoog (MS) medium, apical slices from Scots pine seedlings, which included the base of the cotyledonary whorl subtended by a stub of hypocotyl, were established in culture. Adventitious buds developing on these apical slices were further multiplied at 1-to 2-month intervals. Two plantlets were regenerated when elongated adventitious shoots from these cultures were transferred to a rooting medium. Greater difficulty was encountered when ungerminated embryos were cultured; nevertheless, one plantlet was regenerated using this starting material. The 3 regenerated plantlets were transferred to vermiculite and maintained at high humidity for 2 months, during which time further shoot growth occurred.