Laboratory Methods for the Diagnosis of Viral Diseases

  • Robert L. Atmar
  • Janet A. Englund


The ability of the virology laboratory to document infection with viral agents has increased dramatically over the past decade. Standard virological techniques, such as the propagation of viruses in animals and determination of antibody using complement fixation, have been important historically in the study of the epidemiology, pathology,. and assessment of clinical disease associated with viral infection. Improvements in cell culture techniques and antibody assays now permit the study of viruses and viral disease in many laboratories, not only specialized research laboratories. Viral cultivation techniques utilizing cytokines and other stimulating substances and genetically engineered cell lines or animal strains have enhanced the ability to isolate viruses that were previously unknown or only suspected on clinical grounds. Viruses such as human immunodeficiency virus (HIV), human herpesviruses types 6 and 7, and parvovirus have been better recognized and studied because of these innovations in viral cultivation.


Human Immunodeficiency Virus Influenza Virus Respiratory Syncytial Virus Polymerase Chain Reaction Assay Clinical Specimen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Robert L. Atmar
    • 1
  • Janet A. Englund
    • 2
  1. 1.Acute Viral Respiratory Disease Unit, Departments of Medicine and Microbiology and ImmunologyBaylor College of MedicineHoustonUSA
  2. 2.Acute Viral Respiratory Disease Unit, Departments of Microbiology and Immunology and PediatricsBaylor College of MedicineHoustonUSA

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