CD14 is Expressed by Subsets of Murine Dendritic Cells and Upregulated by Lipopolysaccharide
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The CD14 surface molecule is predominantly expressed by cells of myeloid origin and regarded as a specific marker for macrophages (Mø). Thus, in human mononuclear cell preparations, CD14 expression is a widely used parameter to distinguish Mø from dendritic cells (DC). Since a murine homologue of CD14 was recently identified, this study investigated expression of CD14 by murine Mø and DC. Flow cytometry with a monoclonal antibody directed against murine CD14 revealed that bone marrow-derived DC express CD14 to various extents during differentiation. Functionally, CD14high and CD14low DC did not differ significantly in their capacity to present alloantigen, protein antigen or immunogenic peptide. Furthermore, surface expression of CD14 could be modulated by interleukin (IL)-4 and LPS. Incubation of bone marrow-derived DC with IL-4 (100 U/ml) resulted in downregulation of CD14 surface expression, whereas exposure of BmDC to LPS (1 µg/ml) led to upregulation of CD14. After blockage of CD14 molecules by incubation of DC with anti-CD14 antibodies, downregulation of LPS triggered IL-1 release could be detected. In addition, other Mø markers such as CD11b, F4/80, BM8, and ER-TR9, are also expressed on DC. Therefore, we conclude that CD14, like other Mø markers, is expressed on murine DC during maturation. Thus, Mø and DC cannot be distinguished by flow cytometry using these markers. Moreover, CD14 may be involved in mediating LPS-induced activation of murine DC.
KeywordsDendritic Cell Surface Expression Bone Marrow Culture Mature Dendritic Cell Complete RPMI Medium
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- 9.Inaba, K., Inaba, M., Deguchi, M., Hagi, K., Yasumizu, R., Ikehara, S., Muramatsu, S., Steinman, R.M. (1993) Granulocytes, macrophages, and dendritic cells arise from a common major histocompatibility complex class II-negative progenitor in mouse bone marrow. Proc Natl Acad Sci USA 90, 3038–42.PubMedCrossRefGoogle Scholar
- 11.Caux, C., Vanbervliet, B., Massacrier, C., Dezutter Dambuyant, C., de Saint Vis, B., Jacquet, C., Yoneda, K., Imamura, S., Schmitt, D., Banchereau, J. (1996) CD34’ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF plus TNFa. J. Exp. Med 184, 695–706.PubMedCrossRefGoogle Scholar
- 16.Inaba, K., Inaba, M., Romani, N., Aya, H., Deguchi, M., Ikehara, S., Muramatsu, S., Steinman, R. (1992) Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony stimulating factor. J. Exp. Med. 176, 1693–1702.PubMedCrossRefGoogle Scholar
- 18.Ruppert, J., Peters, J.H. (1991) Accessory cell function during monocyte/macrophage differentiation: relation to interleukin-1 (IL-1 beta) production and release. EurJ Cell Bio1 55, 352–61.Google Scholar
- 20.Sallusto, F., Lanzavecchia, A. (1994) Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and down-regulated by tumor necrosis factor alpha. J. Exp. Med. 179, 1109–18.PubMedCrossRefGoogle Scholar
- 22.Greame-Cook, F., Bhan, A.K., Harris, N.L. (1993) Immunohistochemical characterization of intraepithelial and subepithelial mononuclear cells of the upper airways. Am. J. Pathol. 143, 1416–1422.Google Scholar
- 23.O“Doherty, U., M. Peng, S. Gezelter, W. J. Swiggard, M. Betjes, N. Bhardwaj, and R. M. Steinman. (1994) Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature. Immunology 82, 487–493.Google Scholar