Production of Lines of Growth Enhanced Transgenic Tilapia (Oreochromis Niloticus) Expressing a Novel Piscine Growth Hormone Gene

  • Azirur Rahman
  • Norman Maclean
Chapter

Abstract

Since the first introduction of novel genes in fish (Maclean and Talwar, 1984; Zhu et al., 1985), a wide range of fish species have been used in transgenic research either for commercial or academic purposes. For commercial exploitation this technology has been applied in several commercially important fish species to improve desirable genetic traits such as growth (Rokkones et al., 1989; Zhang et al., 1990; Brem et al., 1988; Penman et al., 1990; Gross et al., 1992), cold tolerance (Fletcher et al., 1988), or disease resistance (Anderson et al., 1996) for aquaculture development. Most of these gene transfer experiment have concentrated on growth enhancement using regulatory sequences and coding sequences of distantly related species (Rokkones et al., 1989; Penman et al., 1990; Brem et al., 1988; Zhang et al., 1990). Although successful gene transfer in these fish was observed in only a few cases has germline transmission and expression of transgenes in progeny been convincingly demonstrated (see reviews by Maclean and Rahman, 1994; Gong and Hew, 1995). In studies where the regulatory sequences used were of mammalian origin, low or nil expression was observed (Guyomard et al., 1989; Alam et al., 1996). In other studies, enhanced growth was observed in transgenic carp (Zhang et al., 1990) and in tilapia (Martinez et al., 1996) when growth hormone sequences were driven by a viral promoter. However, the main objective of the production of transgenic fish with GH gene is to generate novel strain of growth enhanced fish which could subsequently be used in aquaculture. It is, therefore, important that the gene constructs used for producing trans-genic fish be of fish origin, and not from mammalian or viral origin. Moreover, it was also observed that regulatory sequences from fish are found to be more effective than those of mammalian origin in respect to expression of transgenes (Alam et al., 1996) in fish.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alam, M.S., Laveder, F.L., Iyengar, 1; Rahman, M.A.., Ayad, H.H., Lathe, R., Morley, S.D. and Maclean, N. 1996, Mol. Reprod. Dey. 45: 117–122.CrossRefGoogle Scholar
  2. Anderson, E.D., Mourich, D.V., Fahrenkrug, S.C., LaPatra, S., Shepherd, J. and Leong, J.-A.C. 1996, Mol. Mar. Biol. Biotechnol. 5 (2): 114–122.PubMedGoogle Scholar
  3. Bertke, A., Naar, E.M., Johnson, L., May, M.R., Cecim, M., Yun, J.S. and Wagner, T.E. 1992, J. Reprod. Fertil. 95: 109–118CrossRefGoogle Scholar
  4. Brem, G., Brenig, B., Horstgen- Schwark, G. and Winnacker, E. L. 1988, Aquaculture 68: 209–219.CrossRefGoogle Scholar
  5. Cameron, E.R., Harvey, M.J.A. and Onions, D.E. 1994, Transgenic Science. Br. Vet. J. 150: 9–24Google Scholar
  6. Chen, T.T., Lin, C.M., Zhu, Z., Gonzalez-Villasenor, L.I., Dunham, R.A. and Powers, D.A. 1990, In Church, R.B ed., Transgenic Models in Medicine and Agriculture, pp. 127–39. New York: Wiley-LissGoogle Scholar
  7. Devlin, R.H., Yesaki, T.Y., Biagi, C.A., Donaldson, E.M., Swanson, P. and Chan, W. 1994, Nature. 371: 209–10.CrossRefGoogle Scholar
  8. Devlin, R.H., Yesaki, T.Y., Donaldson, E.M., Du, S.J. and Hew, C.L. 1995, Can. J. Fish Aquat. Sci. 52: 1376–1384.CrossRefGoogle Scholar
  9. Du, S.J., Gong, Z., Fletcher, G.L., Shears, M.A., King, M.J., Idler, D.R. and Hew, C.L. 1992, Bio/Technology. 10: 176–81.PubMedCrossRefGoogle Scholar
  10. Fletcher, G.L., Shears, M.A., King, M.J., Davies, P.L. and Hew C.L. 1988, Can.J.Fish. Aquas. Sci. 45: 352–357.CrossRefGoogle Scholar
  11. Gong, Z; Hew, C.L. and Vielkind, J.R., 1991, Mol. Mar. Biol. Biotechnol., 1 (1): 64–72.PubMedGoogle Scholar
  12. Gong, Z. and Hew, C.L. 1995, Current Topics Dev. Biol. 30: 177–214.CrossRefGoogle Scholar
  13. Gross, M.L., Schneider, J.F., Moav, N., Moav, B., Alvarez, C., Myster, S.H., Liu, Z., Hallerman, E.M., Hackett, P.B., Guise, K.S., Faras, A.J. and Kapuscinski, A.R. 1992, Aquaculture 103: 253–73.CrossRefGoogle Scholar
  14. Guyomard, R., Chourrout, D., Lerox, C., Houdebine, L. and Pourrain, F. 1989, Biochimie 71: 857–63PubMedCrossRefGoogle Scholar
  15. Maclean, N. and Talwar, S. 1987, J. Embryol. Expt. Morphol. 82 (suppl): 187Google Scholar
  16. Maclean, N. and Rahman, A. 1994, Transgenic fish. In Maclean N. ed., Animals with novel genes, pp 63–105. Cambridge University Press, USA.Google Scholar
  17. Martinez, R., Estrada, M.P., Berlanga, J., Guillén, I., Hernandez, O., Cabrera, E., Pimentel, R., Morales, R., Herrera, F., Morales, A., Pina, J.C., Abad, J., Sanchez, V., Melamed, R, Lleonart, R. and de la Fuente, J. 1996, Mol. Mar Biol. Biotechnol. 5 (1): 62–70.PubMedGoogle Scholar
  18. Penman, D.J., Beeching, A.J., Penn, S. and Maclean, N. 1990, Aquaculture 85: 35–50.CrossRefGoogle Scholar
  19. Poncin, A. and Rousseau, P. 1994, Societe Europeenne De Biotechnologie Sa, Scientific Rep. no. Rt–001–94–01: PP l–3.Google Scholar
  20. Purse], V.G., Bolt, D.J., Miller, K.F., Pinkert, C.A., Hammer, R.A., Palmiter, R.D. and Brinster, R.L. 1990, J. Re-prod. Fertil. (suppl) 40:235–245.Google Scholar
  21. Rahman, M.A. and Maclean, N. 1992, Aquaculture. 105: 219–32.CrossRefGoogle Scholar
  22. Rahman, M.A., lyengar, I. and Maclean, N. 1997, Transgenic Res. 6: 1–10CrossRefGoogle Scholar
  23. Rexroad, C.E., Maho, K., Bolt, D.J. et al., 1991, J. Anim. Sci. 69: 2995–3004.PubMedGoogle Scholar
  24. Rokkones, E., Alestrom, P., Skjervold, H. and Gautvik, K.M. 1989, J. Comp.Physiol. B 158: 751–758PubMedCrossRefGoogle Scholar
  25. Rosochacki, S.J., Bialowas, H., Czlonkowska, M., Guszkiewicz, A., Kossakowski, M., Sadowska, J., Siadkowska, E., Zebrowska, T., Szumiec, J. and Zwierzchowski, L. 1993, Anim. Sci. papers Reports 11(1): 47–58.Google Scholar
  26. Speare, D.J., Macnair, N. and Hammell, K.L. 1995,. Am. J. Vet. Res. 56(10):1372–1379.Google Scholar
  27. Weatherly, A.H. and Gill, H.S. 1987, Academic Press. London. pp 1–429.Google Scholar
  28. Zhang, P., Hayat, M., Joyce, C., Gonzalez-Villasenor, L.I., Lin, C.M., Dunham, R.A., Chen, T.T. and Powers, D.A. 1990, Mol. Reprod. Del“. 25: 3–13.CrossRefGoogle Scholar
  29. Zhu, Z.; Li, G; He, L. and Chen, S. 1985, Z, angew.lchthyol. 1: 31–34.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Azirur Rahman
    • 1
  • Norman Maclean
    • 1
  1. 1.Division of Cell Science School of Biological Sciences Bassett Crescent EastUniversity of SouthamptonSouthamptonUK

Personalised recommendations