Microarray Technology in Sepsis: Tool or Toy?

  • S. Russwurm
  • H. P. Deigner
  • K. Reinhart
Conference paper

Abstract

Sepsis is a result of highly heterogeneous processes characterized by an involvement of multiple components and their interactions at each organizational level of the human body: genes, cells, tissues, organs. The complexity of the underlying biological and immunological processes has encouraged multiple types of research studies comprising a broad panel of clinical aspects. One of the lessons learned to date is that evaluation of new sepsis therapies has been hampered by fairly unspecific, clinically-based inclusion criteria which insufficiently reflect the molecular mechanisms [1].

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Vincent JL, Angus D, Annane D, et al (2001) Clinical expert round table discussion (session 5) at the Margaux Conference on Critical Illness: outcomes of clinical trials in sepsis: lessons learned. Crit Care Med 29: S136–137PubMedCrossRefGoogle Scholar
  2. 2.
    Southern EM (1974) An improved method for transferring nucleotides from electrophoresis strips to thin layers of ion-exchange cellulose. Anal Biochem 62: 317–318PubMedCrossRefGoogle Scholar
  3. 3.
    Gillespie D, Spiegelman S (1965) A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol 12: 829–842PubMedCrossRefGoogle Scholar
  4. 4.
    Lennon GG, Lehrach H (1991) Hybridization analyses of arrayed cDNA libraries. Trends Genet 7: 314–317PubMedGoogle Scholar
  5. 5.
    Kafatos FC, Jones CW, Efstratiadis A (1979) Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucl Acid Res 7: 15411552Google Scholar
  6. 6.
    Fodor SP, Read JL, Pirrung MC, Stryer L, Lu AT, Solas D (1991) Light-directed, spatially addressable parallel chemical synthesis. Science 251: 767–773PubMedCrossRefGoogle Scholar
  7. 7.
    Pease AC, Solas D, Sullivan EJ, Cronin MT, Holmes CP, Fodor SP (1994) Light-generated oligonucleotide arrays for rapid DNA sequence analysis. Proc Nail Acad Sci USA 91: 50225026Google Scholar
  8. 8.
    Schena M, Shalon D, Davis RW, Brown PO (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270: 467–470PubMedCrossRefGoogle Scholar
  9. 9.
    Edman CF, Raymond DE, Wu DJ, et al (1997) Electric field directed nucleic acid hybridization on microchips. Nucleic Acid Res 25: 4907–4914PubMedCrossRefGoogle Scholar
  10. 10.
    Gray DE, Case-Green SC, Fell TS, Dobson PJ, Southern EM (1997) Ellipsometric and interferometric characterization of DNA probes immobilised on a combinatorial array. Langmuir 13: 2833–2842CrossRefGoogle Scholar
  11. 11.
    Ferguson JA, Boles TC, Adams CP, Walt DR (1996) A fiber-optic DNA biosensor microarray for the analysis of gene expression. Nat Biotechnol 14: 1681–1684PubMedCrossRefGoogle Scholar
  12. 12.
    Michael KL, Taylor LC, Schultz SL, Walt DR (1998) Randomly ordered addressable high-density optical sensor arrays. Anal Chem 70: 1242–1248PubMedCrossRefGoogle Scholar
  13. 13.
    Walt DR (2000) Bead-based fiber-optic arrays. Science 287: 451–452PubMedCrossRefGoogle Scholar
  14. 14.
    Lockhart DJ, Winzeler EA (2000) Genomics, gene expression and DNA arrays. Nature 405: 827–836PubMedCrossRefGoogle Scholar
  15. 15.
    Wu W, Wildsmith SE, Winkley AJ, Yallop R, Elcock FJ, Bugelski PJ (2001) Chemometric strategies for normalization of gene expression data obtained from cDNA microarrays. Analytica Chimica Acta 446: 451–466CrossRefGoogle Scholar
  16. 16.
    Golub TR, Slonim DK, Tamayo P, et al (1999) Molecular classification of cancer: class discovery and class prediction by gene expression monitoring. Science 286: 531–537PubMedCrossRefGoogle Scholar
  17. 17.
    Alizadeh AA, Eisen MB, Davis RE, et al (2000) Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature 403: 503–511PubMedCrossRefGoogle Scholar
  18. 18.
    Varambally S, Dhanasekaran SM, Zhou M, et al (2002) The polycomb group protein EZH2 is involved in progression of prostate cancer. Nature 419: 624–629PubMedCrossRefGoogle Scholar
  19. 19.
    Bakay M, Chen Y-W, Borup R, Zhao P, Nagaraju K, Hoffman EP (2002) Sources of variability and effect of experimental approach on expression profiling data interpretation. BMC Bioinformatics 3: 4PubMedCrossRefGoogle Scholar
  20. 20.
    Joyce DE, Gelbert L, Ciaccia A, DeHoff B, Grinnell BW (2001) Gene expression profile of antithrombotic protein c defines new mechanisms modulating inflammation and apoptosis. J Biol Chem 276: 11199–11203PubMedCrossRefGoogle Scholar
  21. 21.
    Fillion I, Ouellet N, Simard M, Bergeron Y, Sato S, Bergeron MG (2001) Role of chemokines and formyl peptides in pneumococcal pneumonia-induced monocyte/macrophage recruitment. J Immunol 166: 7353–7361PubMedGoogle Scholar
  22. 22.
    Zhao B, Bowden RA, Stavchansky SA, Bowman PD (2001) Human endothelial cell response to gram-negative lipopolysaccharide assessed with cDNA microarrays. Am J Physiol Cell Physiol 281: C1587 - C1595PubMedGoogle Scholar
  23. 23.
    Chinnaiyan AM, Huber-Lang M, Kumar-Sinha C, et al (2001) Molecular signatures of sepsis. Multiorgan gene expression profiles of systemic inflammation. Am J Pal:hol 15: 11991209Google Scholar
  24. 24.
    Drmanac S, Kita D, Labat I, et al (1998) Accurate sequencing by hybridization for DNA diagnostics and individual genomics. Nat Biotechnol 16: 54–58PubMedCrossRefGoogle Scholar
  25. 25.
    Cargill M, Altshuler D, Ireland J, et al (1999) Characterization of single-nucleotide polymorphisms in coding regions of human genes. Nat Genet 22: 231–238PubMedCrossRefGoogle Scholar
  26. 1.
    Vincent JL, Angus D, Annane D, et al (2001) Clinical expert round table discussion (session 5) at the Margaux Conference on Critical Illness: outcomes of clinical trials in sepsis: lessons learned. Crit Care Med 29: S136 - 137 PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • S. Russwurm
  • H. P. Deigner
  • K. Reinhart

There are no affiliations available

Personalised recommendations