New Approaches for Diagnosis of Infections by Intracellular Bacteria

  • Reinhard Marre
Part of the Subcellular Biochemistry book series (SCBI, volume 33)


Laboratory diagnosis of infectious diseases usually is achieved by microscopy, culture, antigen and antibody detection or, in order to overcome diagnostic gaps of single tests, a combination of these methods. During the last years, however, microbiologists increasingly became aware of bacterial species which cannot satisfactorily be detected in a clinical microbiology laboratory by standard methods either due to their low sensitivity and specificity or due to the need to apply expensive and time consuming methods. The introduction of molecular-genetic methods in clinical microbiology seemed to solve many problems: Since speed of detection was no longer dependent on the generation time and on growth conditions of the pathogen and since specific detection of the in vitro amplified DNA or RNA was possible, a new world of non or barely in vitro culturable pathogens appeared. With increasing experience the limits of this technology became obvious, requiring a meticulous quality control program.


Mycobacterium Tuberculosis Chlamydia Trachomatis Neisseria Gonorrhoeae Nucleic Acid Amplification Test Respiratory Specimen 
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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Reinhard Marre
    • 1
  1. 1.Department of Medical Microbiology and HygieneUniversity of UlmUlmGermany

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