In Vitro Studies of Photochemically Cross-Linked Protein-Nucleic Acid Complexes. Determination of Cross-Linked Regions and Structural Relationships in Specific Complexes

  • Paul R. Schimmel
  • Gerald P. Budzik
  • Stella S. M. Lam
  • Hubert J. P. Schoemaker


It is well known that many enzymatic and cellular processes involve specific protein-nucleic acid associations. Examples include the interaction of a repressor with its operator DNA (see Beckwith and Zipser, 1970; Müller-Hill, 1971), of DNA polymerase with DNA (see Kornberg, 1975), of ribosomal proteins with ribosomal RNA (see Nomura et al., 1974), of aminoacyl tRNA synthetases with tRNAs (see Kisselev and Favorova, 1974; Söll and Schimmel, 1974), as well as many other systems. A prime goal is to determine the mechanism by which these interactions occur; viz., it is desirable to understand how specificity is achieved and to determine what factors are responsible for the strength of specific protein-nucleic acid associations.


Aminoacyl tRNA Synthetase Anticodon Loop Cloverleaf Structure Transfer Ribonucleic Acid Isoleucyl tRNA Synthetase 
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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Paul R. Schimmel
    • 1
  • Gerald P. Budzik
    • 1
  • Stella S. M. Lam
    • 1
  • Hubert J. P. Schoemaker
    • 1
  1. 1.Departments of Biology and ChemistryMassachusetts Institute of TechnologyCambridgeUSA

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