Comment on the Assay of Purine Phosphoribosyltransferases in Cultured Human Fibroblasts
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Cultured human skin fibroblasts are commonly utilized in the detection of hemizygosity and heterozygosity for hypoxanthine-guanine phosphoribosyltransferase (HGPRT) deficiency. Two obstacles are encountered in the determination of HGPRT and adenine phosphoribosyltransferase (APRT) in extracts of cultured skin fibroblasts: the sensitivity of these enzymes in dilute cell suspension to freezing and thawing (1), and the presence of nucleotidase activity (2).
KeywordsMicrocrystalline Cellulose Human Skin Fibroblast Purine Metabolism Purine Base Cultured Skin Fibroblast
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